PDB entry 3pqe

View 3pqe on RCSB PDB site
Description: Crystal structure of L-lactate dehydrogenase from Bacillus subtilis with H171C mutation
Class: oxidoreductase
Keywords: L-lactate dehydrogenase, FBP, OXIDOREDUCTASE
Deposited on 2010-11-26, released 2011-12-14
The last revision prior to the SCOPe 2.03 freeze date was dated 2011-12-14, with a file datestamp of 2011-12-09.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.202
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: l-lactate dehydrogenase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: ldh, lctE, BSU03050
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13714
      • engineered mutation (170)
    Domains in SCOPe 2.03: d3pqea1, d3pqea2
  • Chain 'B':
    Compound: l-lactate dehydrogenase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: ldh, lctE, BSU03050
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13714
      • engineered mutation (170)
    Domains in SCOPe 2.03: d3pqeb1, d3pqeb2
  • Chain 'C':
    Compound: l-lactate dehydrogenase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: ldh, lctE, BSU03050
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13714
      • engineered mutation (170)
    Domains in SCOPe 2.03: d3pqec1, d3pqec2
  • Chain 'D':
    Compound: l-lactate dehydrogenase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: ldh, lctE, BSU03050
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13714
      • engineered mutation (170)
    Domains in SCOPe 2.03: d3pqed1, d3pqed2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3pqeA (A:)
mnkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqp
vktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflv
atnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgd
telpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsl
aritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqfl
hsagvlknilkphfaeqkvnhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3pqeA (A:)
nkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqpv
ktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflva
tnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgdt
elpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsla
ritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqflh
sagvlknilkphfa

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3pqeB (B:)
mnkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqp
vktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflv
atnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgd
telpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsl
aritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqfl
hsagvlknilkphfaeqkvnhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3pqeB (B:)
nkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqpv
ktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflva
tnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgdt
elpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsla
ritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqflh
sagvlknilkphfa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3pqeC (C:)
mnkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqp
vktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflv
atnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgd
telpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsl
aritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqfl
hsagvlknilkphfaeqkvnhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3pqeC (C:)
nkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqpv
ktsygtyedckdadivcicaganlelveknlkifkgivsevmasgfdgiflvatnpvdil
tyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgdtelpvwsh
anvggvpvselvekndykqeeldqivddvknaayhiiekkgatyygvamslaritkailh
nensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqflhsagvlkni
lkphf

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3pqeD (D:)
mnkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqp
vktsygtyedckdadivcicaganqkpgetrlelveknlkifkgivsevmasgfdgiflv
atnpvdiltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgd
telpvwshanvggvpvselvekndaykqeeldqivddvknaayhiiekkgatyygvamsl
aritkailhnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqfl
hsagvlknilkphfaeqkvnhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3pqeD (D:)
nkhvnkvaligagfvgssyafalinqgitdelvvidvnkekamgdvmdlnhgkafapqpv
ktsygtyedckdadivcicaganrlelveknlkifkgivsevmasgfdgiflvatnpvdi
ltyatwkfsglpkervigsgttldsarfrfmlseyfgaapqnvcahiigehgdtelpvws
hanvggvpvselvekndykqeeldqivddvknaayhiiekkgatyygvamslaritkail
hnensiltvstyldgqygaddvyigvpavvnrggiagitelnlnekekeqflhsagvlkn
ilkphf