PDB entry 3orz

View 3orz on RCSB PDB site
Description: PDK1 mutant bound to allosteric disulfide fragment activator 2A2
Class: transferase/transferase activator
Keywords: C helix, Ser/Thr-kinase, AGC kinase, allostery, transferase, allosteric activator, bisindolylmaleimide, disulfide, kinase, PDK1, TRANSFERASE-TRANSFERASE ACTIVATOR complex
Deposited on 2010-09-08, released 2011-03-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-04-27, with a file datestamp of 2011-04-22.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.22
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 3-phosphoinositide-dependent protein kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: PDK1, PDPK1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O15530 (Start-315)
      • engineered mutation (104)
    Domains in SCOPe 2.08: d3orza_
  • Chain 'B':
    Compound: 3-phosphoinositide-dependent protein kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: PDK1, PDPK1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O15530 (Start-315)
      • engineered mutation (104)
    Domains in SCOPe 2.08: d3orzb_
  • Chain 'C':
    Compound: 3-phosphoinositide-dependent protein kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: PDK1, PDPK1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O15530 (Start-315)
      • engineered mutation (104)
    Domains in SCOPe 2.08: d3orzc_
  • Chain 'D':
    Compound: 3-phosphoinositide-dependent protein kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: PDK1, PDPK1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O15530 (Start-315)
      • engineered mutation (104)
    Domains in SCOPe 2.08: d3orzd_
  • Heterogens: 2A2, BI4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3orzA (A:)
gamdpefmdgtaaeprpgagslqhaqpppqprkkrpedfkfgkilgegsfstvvlarela
tsreyaikilekrhiikenkvpyvtrerdvmsrldhpffvklyfcfqddeklyfglsyak
ngellkyirkigsfdetctrfytaeivsaleylhgkgiihrdlkpenillnedmhiqitd
fgtakvlspeskqaransfvgtaqyvspellteksackssdlwalgciiyqlvaglppfr
agneylifqkiikleydfpekffpkardlvekllvldatkrlgceemegygplkahpffe
svtwenlhqqtppklt

    Sequence, based on observed residues (ATOM records): (download) 
    >3orzA (A:)
prkkrpedfkfgkilgegsfstvvlarelatsreyaikilekrhiikenkvpyvtrerdv
msrldhpffvklyfcfqddeklyfglsyakngellkyirkigsfdetctrfytaeivsal
eylhgkgiihrdlkpenillnedmhiqitdfgtakvlssfvgtaqyvspellteksacks
sdlwalgciiyqlvaglppfragneylifqkiikleydfpekffpkardlvekllvldat
krlgceemegygplkahpffesvtwenlhqqtppklt

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3orzB (B:)
gamdpefmdgtaaeprpgagslqhaqpppqprkkrpedfkfgkilgegsfstvvlarela
tsreyaikilekrhiikenkvpyvtrerdvmsrldhpffvklyfcfqddeklyfglsyak
ngellkyirkigsfdetctrfytaeivsaleylhgkgiihrdlkpenillnedmhiqitd
fgtakvlspeskqaransfvgtaqyvspellteksackssdlwalgciiyqlvaglppfr
agneylifqkiikleydfpekffpkardlvekllvldatkrlgceemegygplkahpffe
svtwenlhqqtppklt

    Sequence, based on observed residues (ATOM records): (download) 
    >3orzB (B:)
qprkkrpedfkfgkilgegsfstvvlarelatsreyaikilekrhiikenkvpyvtrerd
vmsrldhpffvklyfcfqddeklyfglsyakngellkyirkigsfdetctrfytaeivsa
leylhgkgiihrdlkpenillnedmhiqitdfgtakvlsnsfvgtaqyvspellteksac
kssdlwalgciiyqlvaglppfragneylifqkiikleydfpekffpkardlvekllvld
atkrlgceemegygplkahpffesvtwenlhqqtppklt

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3orzC (C:)
gamdpefmdgtaaeprpgagslqhaqpppqprkkrpedfkfgkilgegsfstvvlarela
tsreyaikilekrhiikenkvpyvtrerdvmsrldhpffvklyfcfqddeklyfglsyak
ngellkyirkigsfdetctrfytaeivsaleylhgkgiihrdlkpenillnedmhiqitd
fgtakvlspeskqaransfvgtaqyvspellteksackssdlwalgciiyqlvaglppfr
agneylifqkiikleydfpekffpkardlvekllvldatkrlgceemegygplkahpffe
svtwenlhqqtppklt

    Sequence, based on observed residues (ATOM records): (download) 
    >3orzC (C:)
prkkrpedfkfgkilgegsfstvvlarelatsreyaikilekrhiikenkvpyvtrerdv
msrldhpffvklyfcfqddeklyfglsyakngellkyirkigsfdetctrfytaeivsal
eylhgkgiihrdlkpenillnedmhiqitdfgtakvlssfvgtaqyvspellteksacks
sdlwalgciiyqlvaglppfragneylifqkiikleydfpekffpkardlvekllvldat
krlgceemegygplkahpffesvtwenlhqqtppklt

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3orzD (D:)
gamdpefmdgtaaeprpgagslqhaqpppqprkkrpedfkfgkilgegsfstvvlarela
tsreyaikilekrhiikenkvpyvtrerdvmsrldhpffvklyfcfqddeklyfglsyak
ngellkyirkigsfdetctrfytaeivsaleylhgkgiihrdlkpenillnedmhiqitd
fgtakvlspeskqaransfvgtaqyvspellteksackssdlwalgciiyqlvaglppfr
agneylifqkiikleydfpekffpkardlvekllvldatkrlgceemegygplkahpffe
svtwenlhqqtppklt

    Sequence, based on observed residues (ATOM records): (download) 
    >3orzD (D:)
qprkkrpedfkfgkilgegsfstvvlarelatsreyaikilekrhiikenkvpyvtrerd
vmsrldhpffvklyfcfqddeklyfglsyakngellkyirkigsfdetctrfytaeivsa
leylhgkgiihrdlkpenillnedmhiqitdfgtakvlsnsfvgtaqyvspellteksac
kssdlwalgciiyqlvaglppfragneylifqkiikleydfpekffpkardlvekllvld
atkrlgceemegygplkahpffesvtwenlhqqtppklt