PDB entry 3ir5

View 3ir5 on RCSB PDB site
Description: Crystal structure of NarGHI mutant NarG-H49C
Class: oxidoreductase
Keywords: Oxidoreductase, Nitrate reductase, electron transfer, membrane protein, 4Fe-4S, Cell membrane, Electron transport, Iron, Iron-sulfur, Membrane, Metal-binding, Molybdenum, Nitrate assimilation, Transport, 3Fe-4S, Cell inner membrane, Formylation, Heme, Transmembrane
Deposited on 2009-08-21, released 2010-01-05
The last revision prior to the SCOPe 2.04 freeze date was dated 2010-04-07, with a file datestamp of 2010-04-02.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.2
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Respiratory nitrate reductase 1 alpha chain
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: narG, bisD, narC, b1224, JW1215
    Database cross-references and differences (RAF-indexed):
    • Uniprot P09152
      • engineered (49)
    Domains in SCOPe 2.04: d3ir5a1, d3ir5a2
  • Chain 'B':
    Compound: Respiratory nitrate reductase 1 beta chain
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: narH, b1225, JW1216
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3ir5b_
  • Chain 'C':
    Compound: Respiratory nitrate reductase 1 gamma chain
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: narI, chlI, b1227, JW1218
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.04: d3ir5c_
  • Heterogens: MD1, 6MO, SF4, AGA, F3S, HEM, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ir5A (A:)
mskfldrfryfkqkgetfadghgqllntnrdwedgyrqrwqhdkivrstcgvnctgscsw
kiyvknglvtwetqqtdyprtrpdlpnheprgcprgasyswylysanrlkypmmrkrlmk
mwreakalhsdpveawasiiedadkaksfkqargrggfvrsswqevneliaasnvytikn
ygpdrvagfspipamsmvsyasgarylsliggtclsfydwycdlppaspqtwgeqtdvpe
sadwynssyiiawgsnvpqtrtpdahfftevrykgtktvavtpdyaeiaklcdlwlapkq
gtdaamalamghvmlrefhldnpsqyftdyvrrytdmpmlvmleerdgyyaagrmlraad
lvdalgqennpewktvafntngemvapngsigfrwgekgkwnleqrdgktgeetelqlsl
lgsqdeiaevgfpyfggdgtehfnkvelenvllhklpvkrlqladgstalvttvydltla
nyglerglndvncatsyddvkaytpawaeqitgvsrsqiiriarefadnadkthgrsmii
vgaglnhwyhldmnyrglinmlifcgcvgqsgggwahyvgqeklrpqtgwqplafaldwq
rparhmnstsyfynhssqwryetvtaeellspmadksrytghlidfnvraermgwlpsap
qlgtnpltiageaekagmnpvdytvkslkegsirfaaeqpengknhprnlfiwrsnllgs
sgkghefmlkyllgtehgiqgkdlgqqggvkpeevdwqdnglegkldlvvtldfrlsstc
lysdiilptatwyekddmntsdmhpfihplsaavdpaweaksdweiykaiakkfsevcvg
hlgketdivtlpiqhdsaaelaqpldvkdwkkgecdlipgktaphimvverdypatyerf
tsigplmekignggkgiawntqsemdllrklnytkaegpakgqpmlntaidaaemiltla
petngqvavkawaalseftgrdhthlalnkedekirfrdiqaqprkiissptwsgledeh
vsynagytnvhelipwrtlsgrqqlyqdhqwmrdfgesllvyrppidtrsvkevigqksn
gnqekalnfltphqkwgihstysdnllmltlgrggpvvwlseadakdlgiadndwievfn
sngaltaravvsqrvpagmtmmyhaqerivnlpgseitqqrggihnsvtritpkpthmig
gyahlaygfnyygtvgsnrdefvvvrkmknidwldgegndqvqesvk

    Sequence, based on observed residues (ATOM records): (download) 
    >3ir5A (A:)
skfldrfryfkqkgetfadghgqllntnrdwedgyrqrwqhdkivrstcgvnctgscswk
iyvknglvtwetqqtdyprtrpdlpnheprgcprgasyswylysanrlkypmmrkrlmkm
wreakalhsdpveawasiiedadkaksfkqargrggfvrsswqevneliaasnvytikny
gpdrvagfspipamsmvsyasgarylsliggtclsfydwycdlppaspqtwgeqtdvpes
adwynssyiiawgsnvpqtrtpdahfftevrykgtktvavtpdyaeiaklcdlwlapkqg
tdaamalamghvmlrefhldnpsqyftdyvrrytdmpmlvmleerdgyyaagrmlraadl
vdalgqennpewktvafntngemvapngsigfrwgekgkwnleqrdgktgeetelqlsll
gsqdeiaevgfpyfggdgtehfnkvelenvllhklpvkrlqladgstalvttvydltlan
yglerglndvncatsyddvkaytpawaeqitgvsrsqiiriarefadnadkthgrsmiiv
gaglnhwyhldmnyrglinmlifcgcvgqsgggwahyvgqeklrpqtgwqplafaldwqr
parhmnstsyfynhssqwryetvtaeellspmadksrytghlidfnvraermgwlpsapq
lgtnpltiageaekagmnpvdytvkslkegsirfaaeqpengknhprnlfiwrsnllgss
gkghefmlkyllgtehgiqgkdlgqqggvkpeevdwqdnglegkldlvvtldfrlsstcl
ysdiilptatwyekddmntsdmhpfihplsaavdpaweaksdweiykaiakkfsevcvgh
lgketdivtlpiqhdsaaelaqpldvkdwkkgecdlipgktaphimvverdypatyerft
sigplmekignggkgiawntqsemdllrklnytkaegpakgqpmlntaidaaemiltlap
etngqvavkawaalseftgrdhthlalnkedekirfrdiqaqprkiissptwsgledehv
synagytnvhelipwrtlsgrqqlyqdhqwmrdfgesllvyrppidtrsvkevigqksng
nqekalnfltphqkwgihstysdnllmltlgrggpvvwlseadakdlgiadndwievfns
ngaltaravvsqrvpagmtmmyhaqerivnlpgseitqqrggihnsvtritpkpthmigg
yahlaygfnyygtvgsnrdefvvvrkmknidwldgegndqvqes

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ir5B (B:)
mkirsqvgmvlnldkcigchtcsvtcknvwtsregveyawfnnvetkpgqgfptdwenqe
kykggwirkingklqprmgnramllgkifanphlpgiddyyepfdfdyqnlhtapegsks
qpiarprslitgermakiekgpnweddlggefdklakdknfdniqkamysqfentfmmyl
prlcehclnpacvatcpsgaiykreedgivlidqdkcrgwrmcitgcpykkiyfnwksgk
sekcifcyprieagqptvcsetcvgrirylgvllydadaieraastenekdlyqrqldvf
ldpndpkvieqaikdgiplsvieaaqqspvykmamewklalplhpeyrtlpmvwyvppls
piqsaadagelgsngilpdveslripvqylanlltagdtkpvlralkrmlamrhykraet
vdgkvdtraleevglteaqaqemyrylaianyedrfvvpsshrelareafpekngcgftf
gdgchgsdtkfnlfnsrridaidvtsktephp

    Sequence, based on observed residues (ATOM records): (download) 
    >3ir5B (B:)
mkirsqvgmvlnldkcigchtcsvtcknvwtsregveyawfnnvetkpgqgfptdwenqe
kykggwirkingklqprmgnramllgkifanphlpgiddyyepfdfdyqnlhtapegsks
qpiarprslitgermakiekgpnweddlggefdklakdknfdniqkamysqfentfmmyl
prlcehclnpacvatcpsgaiykreedgivlidqdkcrgwrmcitgcpykkiyfnwksgk
sekcifcyprieagqptvcsetcvgrirylgvllydadaieraastenekdlyqrqldvf
ldpndpkvieqaikdgiplsvieaaqqspvykmamewklalplhpeyrtlpmvwyvppls
piqsaadagelgsngilpdveslripvqylanlltagdtkpvlralkrmlamrhykraet
vdgkvdtraleevglteaqaqemyrylaianyedrfvvpsshrelareafpekngcgftf
gdgchgsdtkfnlfnsrridaidvtskte

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ir5C (C:)
mqflnmfffdiypyiagavfligswlrydygqytwraassqmldrkgmnlasnlfhigil
gifvghffgmltphwmyeawlpievkqkmamfaggasgvlcliggvlllkrrlfsprvra
tttgadililsllviqcalglltipfsaqhmdgsemmklvgwaqsvvtfhggasqhldgv
afifrlhlvlgmtlfllfpfsrlihiwsvpveyltrkyqlvrarh

    Sequence, based on observed residues (ATOM records): (download) 
    >3ir5C (C:)
mqflnmfffdiypyiagavfligswlrydygqytwraassqmldrkgmnlasnlfhigil
gifvghffgmlteawlpievkqkmamfaggasgvlcliggvlllkrrlfsprvratttga
dililsllviqcalglltipfsaqhmdgsemmklvgwaqsvvtfhggasqhldgvafifr
lhlvlgmtlfllfpfsrlihiwsvpveyltrkyqlvrarh