PDB entry 3ifw

View 3ifw on RCSB PDB site
Description: Crystal structure of the S18Y variant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester.
Class: hydrolase/hydrolase inhibitor
Keywords: Enzyme-suicide substrate complex, Cytoplasm, Disease mutation, Glycoprotein, Hydrolase, Ligase, Oxidation, Polymorphism, Protease, Thiol protease, Ubl conjugation pathway, Isopeptide bond, Nucleus, Phosphoprotein, Ubl conjugation, hydrolase-hydrolase inhibitor COMPLEX
Deposited on 2009-07-26, released 2010-06-16
The last revision prior to the SCOPe 2.06 freeze date was dated 2010-06-16, with a file datestamp of 2010-06-11.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.211
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ubiquitin carboxyl-terminal hydrolase isozyme L1
    Species: Homo sapiens [TaxId:9606]
    Gene: PGP9.5, UCHL1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P09936 (5-227)
      • engineered (22)
    Domains in SCOPe 2.06: d3ifwa_
  • Chain 'B':
    Compound: Ubiquitin
    Species: Homo sapiens [TaxId:9606]
    Gene: RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBIQ_HUMAN
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3ifwb_
  • Heterogens: GVE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3ifwA (A:)
    gplgsmqlkpmeinpemlnkvlyrlgvagqwrfvdvlgleeeslgsvpapacallllfpl
    taqhenfrkkqieelkgqevspkvyfmkqtignscgtiglihavannqdklgfedgsvlk
    qflsetekmspedrakcfekneaiqaahdavaqegqcrvddkvnfhfilfnnvdghlyel
    dgrmpfpvnhgassedtllkdaakvcreftereqgevrfsavalckaa
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ifwA (A:)
    mqlkpmeinpemlnkvlyrlgvagqwrfvdvlgleeeslgsvpapacallllfpltaqhe
    nfrkkqieelkgqevspkvyfmkqtignscgtiglihavannqdklgfedgsvlkqflse
    tekmspedrakcfekneaiqaahdavaqegqcrvddkvnfhfilfnnvdghlyeldgrmp
    fpvnhgassedtllkdaakvcreftereqgevrfsavalckaa
    

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3ifwB (B:)
    mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
    iqkestlhlvlrlrg