PDB entry 3ifa

View 3ifa on RCSB PDB site
Description: Human muscle fructose-1,6-bisphosphatase E69Q mutant in complex with AMP
Class: hydrolase
Keywords: gluconeogenesis, glyconeogenesis, muscle fructose-1, 6-bisphosphatase, protein engineering, calcium inhibition, Allosteric enzyme, Carbohydrate metabolism, Hydrolase, Magnesium, Metal-binding, Phosphoprotein
Deposited on 2009-07-24, released 2010-08-11
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-11-01, with a file datestamp of 2017-10-27.
Experiment type: XRAY
Resolution: 1.93 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.08: d3ifaa_
  • Chain 'B':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.08: d3ifab_
  • Chain 'C':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.08: d3ifac_
  • Chain 'D':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • see remark 999 (84)
    Domains in SCOPe 2.08: d3ifad_
  • Heterogens: AMP, SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ifaA (A:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifaA (A:)
tdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagsvnvdq
vkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidcl
asigtifaiyrktsdepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpal
gefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmva
dvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeai
hqrvplilgspedvqeyltcvqknqa

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ifaB (B:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifaB (B:)
tdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagqvkkld
vlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasigt
ifaiyrktsdepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefvl
vekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhrt
lvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrvp
lilgspedvqeyltcvqknqa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ifaC (C:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifaC (C:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagsvqvkkl
dvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasig
tifaiyrktsdepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefv
lvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhr
tlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrv
plilgspedvqeyltcvqknq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3ifaD (D:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifaD (D:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagsvnvvkk
ldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasi
gtifaiyrktsepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefv
lvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhr
tlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrv
plilgspedvqeyltcvqknqa