PDB entry 3fsu

View 3fsu on RCSB PDB site
Description: Crystal Structure of Escherichia coli Methylenetetrahydrofolate Reductase Double Mutant Phe223LeuGlu28Gln complexed with methyltetrahydrofolate
Class: oxidoreductase
Keywords: TIM BARREL, FLAVIN, REDUCTASE, METHYLTETRAHYDROFOLATE, Amino-acid biosynthesis, FAD, Flavoprotein, Methionine biosynthesis, NAD, NADP, Oxidoreductase
Deposited on 2009-01-12, released 2009-08-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-08-25, with a file datestamp of 2009-08-21.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.198
AEROSPACI score: 0.54 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: metF, b3941, JW3913
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (27)
      • engineered (222)
    Domains in SCOPe 2.08: d3fsua_
  • Chain 'C':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: metF, b3941, JW3913
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (27)
      • engineered (222)
    Domains in SCOPe 2.08: d3fsuc_
  • Chain 'E':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: metF, b3941, JW3913
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (27)
      • engineered (222)
    Domains in SCOPe 2.08: d3fsue_
  • Heterogens: FAD, C2F, MRY, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3fsuA (A:)
msffhasqrdalnqslaevqgqinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdaganra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkladmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3fsuA (A:)
ffhasqrdalnqslaevqgqinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvtyga
nsgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgdle
myasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdaganraitqfffdve
sylrfrdrcvsagidveiipgilpvsnfkqakkladmtnvripawmaqmfdgldddaetr
klvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3fsuC (C:)
msffhasqrdalnqslaevqgqinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdaganra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkladmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3fsuC (C:)
qinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvtygansgerdrthsiikgikdrt
gleaaphltcidatpdelrtiardywnngirhivalrgdlemyasdlvtllkevadfdis
vaaypevhpeaksaqadllnlkrkvdaganraitqfffdvesylrfrdrcvsagidveii
pgilpvsnfkqakkladmtnvripawmaqmfdgldddaetrklvganiamdmvkilsreg
vkdfhfytlnraemsyaichtlgvrp

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3fsuE (E:)
msffhasqrdalnqslaevqgqinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdaganra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkladmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3fsuE (E:)
ffhasqrdalnqslaevqgqinvsfqffpprtsemeqtlwnsidrlsslkpkfvsvtyth
siikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgdlppemyasdlv
tllkevadfdisvaaypevhpeaksaqadllnlkrkvdaganraitqfffdvesylrfrd
rcvsagidveiipgilpvsnfkqakkladmtnvripawmaqmfdgldddaetrklvgani
amdmvkilsregvkdfhfytlnraemsyaichtlgvrp