PDB entry 3ew4

View 3ew4 on RCSB PDB site
Description: Alginate lyase a1-III Y246F complexed with alginate tetrasaccharide
Class: Lyase
Keywords: alginate lyase, tetrasaccharide complex, alpha barrel, Lyase
Deposited on 2008-10-14, released 2009-10-20
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-04-11, with a file datestamp of 2012-04-06.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.162
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Alginate lyase
    Species: Sphingomonas sp. [TaxId:28214]
    Gene: ALY
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KWU1 (2-352)
      • expression tag (0-1)
      • engineered (242)
      • conflict (343)
    Domains in SCOPe 2.08: d3ew4a1, d3ew4a2
  • Chain 'B':
    Compound: Alginate lyase
    Species: Sphingomonas sp. [TaxId:28214]
    Gene: ALY
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KWU1 (2-352)
      • expression tag (0-1)
      • engineered (242)
      • conflict (343)
    Domains in SCOPe 2.08: d3ew4b1, d3ew4b2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3ew4A (A:)
gshpfdqavvkdptasyvdvkarrtflqsgqlddrlkaalpkeydctteatpnpqqgemv
iprrylsgnhgpvnpdyepvvtlyrdfekisatlgnlyvatgkpvyatcllnmldkwaka
dallnydpksqswyqvewsaataafalstmmaepnvdtaqrervvkwlnrvarhqtsfpg
gdtsccnnhsywrgqeatiigviskddelfrwglgryvqamglinedgsfvhemtrheqs
lhfqnyamlpltmiaetasrqgidlyaykengrdihsarkfvfaavknpdlikkyasepq
dtrafkpgrgdlnwieyqrarfgfadelgfmtvpifdprtggsatllaykpqg

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3ew4B (B:)
gshpfdqavvkdptasyvdvkarrtflqsgqlddrlkaalpkeydctteatpnpqqgemv
iprrylsgnhgpvnpdyepvvtlyrdfekisatlgnlyvatgkpvyatcllnmldkwaka
dallnydpksqswyqvewsaataafalstmmaepnvdtaqrervvkwlnrvarhqtsfpg
gdtsccnnhsywrgqeatiigviskddelfrwglgryvqamglinedgsfvhemtrheqs
lhfqnyamlpltmiaetasrqgidlyaykengrdihsarkfvfaavknpdlikkyasepq
dtrafkpgrgdlnwieyqrarfgfadelgfmtvpifdprtggsatllaykpqg