PDB entry 3bsu

View 3bsu on RCSB PDB site
Description: Hybrid-binding domain of human RNase H1 in complex with 12-mer RNA/DNA
Class: hydrolase/RNA/DNA
Keywords: RNase H, RNA/DNA hybrid, dsRNA, HYDROLASE/RNA/DNA COMPLEX
Deposited on 2007-12-26, released 2008-03-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-20.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.22
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O60930
      • cloning artifact (2)
    Domains in SCOPe 2.08: d3bsua1, d3bsua2
  • Chain 'B':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O60930
      • cloning artifact (2)
    Domains in SCOPe 2.08: d3bsub1, d3bsub2
  • Chain 'C':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3bsuc_
  • Chain 'D':
    Compound: RNA (5'-r(*gp*ap*cp*ap*cp*cp*up*gp*ap*up*up*c)-3')
  • Chain 'E':
    Compound: DNA (5'-d(*dgp*dap*dap*dtp*dcp*dap*dgp*dgp*(5iu)p*dgp*dtp*dc)-3')
  • Chain 'F':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O60930
      • cloning artifact (1-2)
    Domains in SCOPe 2.08: d3bsuf1, d3bsuf2
  • Chain 'G':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O60930 (0-End)
      • cloning artifact (1-2)
    Domains in SCOPe 2.08: d3bsug_
  • Chain 'H':
    Compound: Ribonuclease H1
    Species: Homo sapiens [TaxId:9606]
    Gene: RNASEH1, RNH1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O60930
      • cloning artifact (2)
    Domains in SCOPe 2.08: d3bsuh1, d3bsuh2
  • Chain 'I':
    Compound: RNA (5'-r(*gp*ap*cp*ap*cp*cp*up*gp*ap*up*up*c)-3')
  • Chain 'J':
    Compound: DNA (5'-d(*dgp*dap*dap*dtp*dcp*dap*dgp*dgp*(5iu)p*dgp*dtp*dc)-3')
  • Heterogens: MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3bsuA (A:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuA (A:)
hmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3bsuB (B:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuB (B:)
hmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3bsuC (C:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuC (C:)
mfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrks

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3bsuF (F:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuF (F:)
shmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrk

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3bsuG (G:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuG (G:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrk

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >3bsuH (H:)
gshmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvrksas

    Sequence, based on observed residues (ATOM records): (download) 
    >3bsuH (H:)
hmfyavrrgrktgvfltwnecraqvdrfpaarfkkfatedeawafvr

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.