PDB entry 2zaf

View 2zaf on RCSB PDB site
Description: Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase
Class: oxidoreductase
Keywords: oxidoreductase, flavoenzyme, nitroalkane, flavoprotein
Deposited on 2007-10-05, released 2008-06-03
The last revision prior to the SCOPe 2.05 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.23
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: nitroalkane oxidase
    Species: Fusarium oxysporum
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8X1D8
      • engineered (408)
    Domains in SCOPe 2.05: d2zafa1, d2zafa2
  • Chain 'B':
    Compound: nitroalkane oxidase
    Species: Fusarium oxysporum
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8X1D8
      • engineered (408)
    Domains in SCOPe 2.05: d2zafb1, d2zafb2
  • Chain 'C':
    Compound: nitroalkane oxidase
    Species: Fusarium oxysporum
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8X1D8
      • engineered (408)
    Domains in SCOPe 2.05: d2zafc1, d2zafc2
  • Chain 'D':
    Compound: nitroalkane oxidase
    Species: Fusarium oxysporum
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8X1D8
      • engineered (408)
    Domains in SCOPe 2.05: d2zafd1, d2zafd2
  • Heterogens: FAD

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2zafA (A:)
mvdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglika
qvpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpf
isgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykg
adlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitt
sgphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfak
sdtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtki
yttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmale
dyepwaatygsskvdksrl

    Sequence, based on observed residues (ATOM records): (download) 
    >2zafA (A:)
vdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglikaq
vpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpfi
sgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykga
dlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitts
gphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfaks
dtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtkiy
ttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmaled
yepwaatygs

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2zafB (B:)
mvdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglika
qvpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpf
isgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykg
adlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitt
sgphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfak
sdtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtki
yttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmale
dyepwaatygsskvdksrl

    Sequence, based on observed residues (ATOM records): (download) 
    >2zafB (B:)
vdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglikaq
vpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpfi
sgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykga
dlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitts
gphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfaks
dtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtkiy
ttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmaled
yepwaatygs

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2zafC (C:)
mvdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglika
qvpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpf
isgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykg
adlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitt
sgphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfak
sdtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtki
yttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmale
dyepwaatygsskvdksrl

    Sequence, based on observed residues (ATOM records): (download) 
    >2zafC (C:)
vdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglikaq
vpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpfi
sgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykga
dlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitts
gphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfaks
dtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtkiy
ttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmaled
yepwaatygs

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2zafD (D:)
mvdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglika
qvpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpf
isgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykg
adlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitt
sgphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfak
sdtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtki
yttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmale
dyepwaatygsskvdksrl

    Sequence, based on observed residues (ATOM records): (download) 
    >2zafD (D:)
vdfklspsqlearrhaqafantvltkasaeystqkdqlsrfqatrpfyreavrhglikaq
vpiplggtmeslvhesiileelfavepatsitivatalglmpvilcdspslqekflkpfi
sgegeplaslmhsepngtanwlqkggpglqttarkvgnewvisgeklwpsnsggwdykga
dlacvvcrvsddpskpqdpnvdpatqiavllvtretiannkkdayqilgepelaghitts
gphtrftefhvphenllctpglkaqglvetafamsaalvgamaigtaraafeealvfaks
dtrggskhiiehqsvadklidckirletsrllvwkavttledealewkvklemamqtkiy
ttdvavecvidamkavgmksyakdmsfprllnevmcyplfdggniglkrrqmqrvmaled
yepwaatygs