PDB entry 2wbk

View 2wbk on RCSB PDB site
Description: Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis
Class: hydrolase
Keywords: glycoside hydrolase, conformational itinerary, hydrolase, mannosidase, beta-mannoside, michaelis complex
Deposited on 2009-03-02, released 2009-03-17
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-04-02, with a file datestamp of 2014-03-28.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.172
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-mannosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8AAK6 (Start-838)
      • engineered mutation (529)
    • PDB 2WBK
    Domains in SCOPe 2.06: d2wbka1, d2wbka2, d2wbka3, d2wbka4, d2wbka5
  • Chain 'B':
    Compound: beta-mannosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8AAK6 (Start-838)
      • engineered mutation (529)
    • PDB 2WBK (839-End)
    Domains in SCOPe 2.06: d2wbkb1, d2wbkb2, d2wbkb3, d2wbkb4, d2wbkb5, d2wbkb6
  • Heterogens: EDO, CL, M2F, BR, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wbkA (A:)
qgndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwve
nedweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvl
rkgenhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmv
tsgvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvsln
gttvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqs
hriglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmk
eanmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavyni
rrlrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefds
drfyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsqfgfqsfpemk
tiaafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgm
rhgleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiq
qndslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldg
wltpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlf
ssmlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretykl
ehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2wbkA (A:)
ndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwvene
dweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvlrk
genhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmvts
gvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvslngt
tvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqshr
iglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmkea
nmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavynirr
lrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefdsdr
fyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsqfgfqsfpemkti
aafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgmrh
gleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiqqn
dslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldgwl
tpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlfss
mlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretyk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wbkB (B:)
qgndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwve
nedweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvl
rkgenhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmv
tsgvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvsln
gttvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqs
hriglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmk
eanmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavyni
rrlrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefds
drfyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsqfgfqsfpemk
tiaafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgm
rhgleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiq
qndslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldg
wltpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlf
ssmlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretykl
ehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2wbkB (B:)
gndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwven
edweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvlr
kgenhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmvt
sgvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvslng
ttvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqsh
riglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmke
anmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavynir
rlrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefdsd
rfyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsqfgfqsfpemkt
iaafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgmr
hgleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiqq
ndslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldgw
ltpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlfs
smlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretykle