PDB entry 2vgf

View 2vgf on RCSB PDB site
Description: human erythrocyte pyruvate kinase: t384m mutant
Class: transferase
Keywords: metal-binding, phosphorylation, r-state, glycolysis, transferase
Deposited on 2007-11-12, released 2007-11-20
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-12-07, with a file datestamp of 2011-12-02.
Experiment type: XRAY
Resolution: 2.75 Å
R-factor: 0.269
AEROSPACI score: 0.2 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pyruvate kinase isozymes r/l
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30613
      • engineered mutation (337)
    Domains in SCOPe 2.04: d2vgfa1, d2vgfa2, d2vgfa3
  • Chain 'B':
    Compound: pyruvate kinase isozymes r/l
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30613
      • engineered mutation (337)
    Domains in SCOPe 2.04: d2vgfb1, d2vgfb2, d2vgfb3
  • Chain 'C':
    Compound: pyruvate kinase isozymes r/l
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30613
      • engineered mutation (337)
    Domains in SCOPe 2.04: d2vgfc1, d2vgfc2, d2vgfc3
  • Chain 'D':
    Compound: pyruvate kinase isozymes r/l
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30613
      • engineered mutation (337)
    Domains in SCOPe 2.04: d2vgfd1, d2vgfd2, d2vgfd3
  • Heterogens: FBP, PGA, K, MN

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2vgfA (A:)
ltqelgtaffqqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlke
mikagmniarlnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgi
lqggpesevelvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglisl
vvqkigpeglvtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfas
fvrkasdvaavraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipa
ekvflaqkmmigrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsg
etakgnfpveavkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkc
caaaiivltttgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwa
ddvdrrvqfgiesgklrgflrvgdlvivvtgwrpgsgytnimrvlsis

    Sequence, based on observed residues (ATOM records): (download) 
    >2vgfA (A:)
qqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlkemikagmniar
lnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgilqggpeseve
lvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglislvvqkigpegl
vtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfasfvrkasdvaa
vraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipaekvflaqkmm
igrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsgetakgnfpve
avkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkccaaaiivltt
tgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwaddvdrrvqfg
iesgklrgflrvgdlvivvtgwrpgsgytnimrvlsi

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2vgfB (B:)
ltqelgtaffqqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlke
mikagmniarlnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgi
lqggpesevelvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglisl
vvqkigpeglvtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfas
fvrkasdvaavraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipa
ekvflaqkmmigrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsg
etakgnfpveavkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkc
caaaiivltttgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwa
ddvdrrvqfgiesgklrgflrvgdlvivvtgwrpgsgytnimrvlsis

    Sequence, based on observed residues (ATOM records): (download) 
    >2vgfB (B:)
qqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlkemikagmniar
lnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgivelvkgsqvl
vtantvwvdypnivrvvpvggriyiddglislvvvtqvenggvlgsrkgvnlpgaqvdlp
glseqdvrdlrfgvehgvdivfasfvrkasdvaavraalgpeghgikiiskienhegvkr
fdeilevsdgimvargdlgieipaekvflaqkmmigrcnlagkpvvcatqmlesmitkpr
pmraetsdvanavldgadcimlsgetakgnfpveavkmqhaiareaeaavyhrqlfeelr
raaplsrdptevtaigaveaafkccaaaiivltttgrsaqllsryrpraaviavtrsaqa
arqvhlcrgvfpllyreppeaiwaddvdrrvqfgiesgklrgflrvgdlvivvtgwrpgs
gytnimrvlsi

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2vgfC (C:)
ltqelgtaffqqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlke
mikagmniarlnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgi
lqggpesevelvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglisl
vvqkigpeglvtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfas
fvrkasdvaavraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipa
ekvflaqkmmigrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsg
etakgnfpveavkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkc
caaaiivltttgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwa
ddvdrrvqfgiesgklrgflrvgdlvivvtgwrpgsgytnimrvlsis

    Sequence, based on observed residues (ATOM records): (download) 
    >2vgfC (C:)
qqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlkemikagmniar
lnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgilqggpeseve
lvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglislvvqkigpegl
vtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfasfvrkasdvaa
vraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipaekvflaqkmm
igrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsgetakgnfpve
avkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkccaaaiivltt
tgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwaddvdrrvqfg
iesgklrgflrvgdlvivvtgwrpgsgytnimrvlsi

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2vgfD (D:)
ltqelgtaffqqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlke
mikagmniarlnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgi
lqggpesevelvkgsqvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglisl
vvqkigpeglvtqvenggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfas
fvrkasdvaavraalgpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipa
ekvflaqkmmigrcnlagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsg
etakgnfpveavkmqhaiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkc
caaaiivltttgrsaqllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwa
ddvdrrvqfgiesgklrgflrvgdlvivvtgwrpgsgytnimrvlsis

    Sequence, based on observed residues (ATOM records): (download) 
    >2vgfD (D:)
qqqqlpaamadtflehlclldidsepvaarstsiiatigpasrsverlkemikagmniar
lnfshgsheyhaesianvreavesfagsplsyrpvaialdtkgpeirtgpesevelvkgs
qvlvtvdpafrtrgnantvwvdypnivrvvpvggriyiddglislvvqkigpeglvtqve
nggvlgsrkgvnlpgaqvdlpglseqdvrdlrfgvehgvdivfasfvrkasdvaavraal
gpeghgikiiskienhegvkrfdeilevsdgimvargdlgieipaekvflaqkmmigrcn
lagkpvvcatqmlesmitkprpmraetsdvanavldgadcimlsgetakgnfpveavkmq
haiareaeaavyhrqlfeelrraaplsrdptevtaigaveaafkccaaaiivltttgrsa
qllsryrpraaviavtrsaqaarqvhlcrgvfpllyreppeaiwaddvdrrvqfgiesgk
lrgflrvgdlvivvtgwrpgsgytnimrvlsi