PDB entry 2px8

View 2px8 on RCSB PDB site
Description: Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH and 7M-GTP
Class: transferase
Keywords: Murray Valley Encephalitis Virus, Methyltransferase, SAH, 7M-GTP, 3PO, Structural Genomics, Oxford Protein Production Facility, OPPF, TRANSFERASE
Deposited on 2007-05-14, released 2007-05-29
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.169
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Genome polyprotein [Contains: Capsid protein C (Core protein); Envelope protein M (Matrix protein); Major envelope protein E; Non-structural protein 1 (NS1); Non-structural protein 2A (NS2A); Flavivirin protease NS2B regulatory subunit; Flavivirin protease NS3 catalytic subunit; Non-structural protein 4A (NS4A); Non-structural protein 4B (NS4B); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5)]
    Species: Murray valley encephalitis virus (strain MVE-1-51) [TaxId:301478]
    Gene: NS5
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05769
      • modified residue (14)
      • modified residue (75)
      • modified residue (94)
      • modified residue (104)
    Domains in SCOPe 2.08: d2px8a_
  • Chain 'B':
    Compound: Genome polyprotein [Contains: Capsid protein C (Core protein); Envelope protein M (Matrix protein); Major envelope protein E; Non-structural protein 1 (NS1); Non-structural protein 2A (NS2A); Flavivirin protease NS2B regulatory subunit; Flavivirin protease NS3 catalytic subunit; Non-structural protein 4A (NS4A); Non-structural protein 4B (NS4B); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5)]
    Species: Murray valley encephalitis virus (strain MVE-1-51) [TaxId:301478]
    Gene: NS5
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05769 (Start-268)
      • modified residue (14)
      • modified residue (75)
      • modified residue (94)
      • modified residue (104)
    Domains in SCOPe 2.08: d2px8b_
  • Heterogens: CL, MGT, 3PO, SAH, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2px8A (A:)
graggrtlgeqwkeklnamgkeeffsyrkeailevdrtearrarregnkvgghpvsrgta
klrwlverrfvqpigkvvdlgcgrggwsyyaatmknvqevrgytkggpgheepmlmqsyg
wnivtmksgvdvfykpseisdtllcdigesspsaeieeqrtlrilemvsdwlsrgpkefc
ikilcpympkviekleslqrrfggglvrvplsrnsnhemywvsgasgnivhavnmtsqvl
igrmdkkiwkgpkyeedvnlgsgtravgk

    Sequence, based on observed residues (ATOM records): (download) 
    >2px8A (A:)
ggrtlgeqwkeklnamgkeeffsyrkeailevdrtearrarregnkvgghpvsrgtaklr
wlverrfvqpigkvvdlgcgrggwsyyaatmknvqevrgytkggpgheepmlmqsygwni
vtmksgvdvfykpseisdtllcdigesspsaeieeqrtlrilemvsdwlsrgpkefciki
lcpympkviekleslqrrfggglvrvplsrnsnhemywvsgasgnivhavnmtsqvligr
mdkkiwkgpkyeedvnlgsgtrav

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2px8B (B:)
graggrtlgeqwkeklnamgkeeffsyrkeailevdrtearrarregnkvgghpvsrgta
klrwlverrfvqpigkvvdlgcgrggwsyyaatmknvqevrgytkggpgheepmlmqsyg
wnivtmksgvdvfykpseisdtllcdigesspsaeieeqrtlrilemvsdwlsrgpkefc
ikilcpympkviekleslqrrfggglvrvplsrnsnhemywvsgasgnivhavnmtsqvl
igrmdkkiwkgpkyeedvnlgsgtravgk

    Sequence, based on observed residues (ATOM records): (download) 
    >2px8B (B:)
grtlgeqwkeklnamgkeeffsyrkeailevdrtearrarregnkvgghpvsrgtaklrw
lverrfvqpigkvvdlgcgrggwsyyaatmknvqevrgytkggpgheepmlmqsygwniv
tmksgvdvfykpseisdtllcdigesspsaeieeqrtlrilemvsdwlsrgpkefcikil
cpympkviekleslqrrfggglvrvplsrnsnhemywvsgasgnivhavnmtsqvligrm
dkkiwkgpkyeedvnlgsgtravgk