PDB entry 2pwf

View 2pwf on RCSB PDB site
Description: Crystal structure of the MutB D200A mutant in complex with glucose
Class: isomerase
Keywords: TREHALULOSE SYNTHASE, SUCROSE ISOMERASE, ALPHA-AMYLASE FAMILY, (BETA/ALPHA)8, BARREL, enzyme complex, Isomerase
Deposited on 2007-05-11, released 2007-06-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: N/A
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Sucrose isomerase
    Species: Pseudomonas mesoacidophila [TaxId:265293]
    Gene: mutB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2PS28 (Start-555)
      • engineered (198)
    Domains in SCOPe 2.08: d2pwfa1, d2pwfa2
  • Chain 'B':
    Compound: Sucrose isomerase
    Species: Pseudomonas mesoacidophila [TaxId:265293]
    Gene: mutB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2PS28 (Start-555)
      • engineered (198)
    Domains in SCOPe 2.08: d2pwfb1, d2pwfb2
  • Chain 'C':
    Compound: Sucrose isomerase
    Species: Pseudomonas mesoacidophila [TaxId:265293]
    Gene: mutB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2PS28 (0-555)
      • engineered (198)
    Domains in SCOPe 2.08: d2pwfc1, d2pwfc2
  • Chain 'D':
    Compound: Sucrose isomerase
    Species: Pseudomonas mesoacidophila [TaxId:265293]
    Gene: mutB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2PS28 (0-555)
      • engineered (198)
    Domains in SCOPe 2.08: d2pwfd1, d2pwfd2
  • Heterogens: BGC, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2pwfA (A:)
pgapwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntd
ngydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdn
pyrdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklree
lyamlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhe
kvfdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtlad
frqtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtp
fifqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpf
qwddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsy
rdidpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaaga
aslelqpwqsgiykvk

    Sequence, based on observed residues (ATOM records): (download) 
    >2pwfA (A:)
gapwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntdn
gydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdnp
yrdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklreel
yamlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhek
vfdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtladf
rqtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtpf
ifqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpfq
wddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsyr
didpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaagaa
slelqpwqsgiykvk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2pwfB (B:)
pgapwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntd
ngydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdn
pyrdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklree
lyamlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhe
kvfdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtlad
frqtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtp
fifqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpf
qwddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsy
rdidpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaaga
aslelqpwqsgiykvk

    Sequence, based on observed residues (ATOM records): (download) 
    >2pwfB (B:)
apwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntdng
ydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdnpy
rdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklreely
amlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhekv
fdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtladfr
qtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtpfi
fqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpfqw
ddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsyrd
idpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaagaas
lelqpwqsgiykvk

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2pwfC (C:)
pgapwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntd
ngydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdn
pyrdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklree
lyamlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhe
kvfdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtlad
frqtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtp
fifqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpf
qwddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsy
rdidpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaaga
aslelqpwqsgiykvk

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2pwfD (D:)
pgapwwksavfyqvyprsfkdtngdgigdfkgltekldylkglgidaiwinphyaspntd
ngydisdyrevmkeygtmedfdrlmaelkkrgmrlmvdvvinhssdqhewfkssraskdn
pyrdyyfwrdgkdghepnnypsffggsawekdpvtgqyylhyfgrqqpdlnwdtpklree
lyamlrfwldkgvsgmrfatvatysktpgfpdltpeqmknfaeaytqgpnlhrylqemhe
kvfdhydavtageifgaplnqvplfidsrrkeldmaftfdlirydraldrwhtiprtlad
frqtidkvdaiageygwntfflgnhdnpravshfgddrpqwreasakalatvtltqrgtp
fifqgdelgmtnypfktlqdfddievkgffqdyvetgkataeelltnvaltsrdnartpf
qwddsanagfttgkpwlkvnpnyteinaareigdpksvysfyrnlisirhetpalstgsy
rdidpsnadvyaytrsqdgetylvvvnfkaeprsftlpdgmhiaetliessspaapaaga
aslelqpwqsgiykvk