PDB entry 2puf

View 2puf on RCSB PDB site
Description: crystal structure of the laci family member, purr, bound to DNA: minor groove binding by alpha helices
Class: transcription/DNA
Keywords: complex (DNA-binding protein-DNA), DNA-binding regulatory protein, extended corepressor specificity, transcription-DNA complex
Deposited on 1997-10-04, released 1998-05-06
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-04-04, with a file datestamp of 2018-03-29.
Experiment type: XRAY
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.12 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: protein (purine repressor)
    Species: Escherichia coli [TaxId:562]
    Gene: PURR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0ACP7
      • engineered (188)
    Domains in SCOPe 2.08: d2pufa1, d2pufa2
  • Chain 'B':
    Compound: DNA (5'-d(*tp*ap*cp*gp*cp*ap*ap*ap*cp*gp*tp*tp*tp*gp*cp*gp*t )-3')
  • Heterogens: GUN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2pufA (A:)
atikdvakranvstttvshvinktrfvaeetrnavwaaikelhyspsavarslkvnhtks
igllatsseaayfaeiieavekncfqkgytlilgnawnnlekqraylsmmaqkrvdgllv
mcseypepllamleeyrhipmvvmdwgeakadftdavidnafeggymagrylierghrei
gvipgpleqntgagrlagfmkameeamikvpeswivqgdfepesgyramqqilsqphrpt
avfcggdimamgalcaademglrvpqdvsligydnvrnaryftpalttihqpkdslgeta
fnmlldrivnkreepqsievhprlierrsvadgpfrdyrr

    Sequence, based on observed residues (ATOM records): (download) 
    >2pufA (A:)
tikdvakranvstttvshvinktrfvaeetrnavwaaikelhyspsavarslkvnhtksi
gllatsseaayfaeiieavekncfqkgytlilgnawnnlekqraylsmmaqkrvdgllvm
cseypepllamleeyrhipmvvmdwgeakadftdavidnafeggymagrylierghreig
vipgpleqntgagrlagfmkameeamikvpeswivqgdfepesgyramqqilsqphrpta
vfcggdimamgalcaademglrvpqdvsligydnvrnaryftpalttihqpkdslgetaf
nmlldrivnkreepqsievhprlierrsvadgpfrdyr

  • Chain 'B':
    No sequence available.