PDB entry 2oip

View 2oip on RCSB PDB site
Description: Crystal Structure of the S290G Active Site Mutant of TS-DHFR from Cryptosporidium hominis
Class: transferase, oxidoreductase
Keywords: bifunctional enzyme, transferase, oxidoreductase
Deposited on 2007-01-11, released 2007-07-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-18, with a file datestamp of 2017-10-13.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-518)
      • engineered (287)
    Domains in SCOPe 2.08: d2oipa1, d2oipa2
  • Chain 'B':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-518)
      • engineered (287)
    Domains in SCOPe 2.08: d2oipb1, d2oipb2
  • Chain 'C':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-518)
      • engineered (287)
    Domains in SCOPe 2.08: d2oipc1, d2oipc2
  • Chain 'D':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-518)
      • engineered (287)
    Domains in SCOPe 2.08: d2oipd1, d2oipd2
  • Chain 'E':
    Compound: Chain A, crystal structure of Dhfr
    Species: Cryptosporidium hominis [TaxId:237895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-518)
      • engineered (287)
    Domains in SCOPe 2.08: d2oipe1, d2oipe2
  • Heterogens: UMP, CB3, MTX, NDP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2oipA (A:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
lqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyld
llsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgd
tngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytg
vgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyq
rscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtpr
pfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >2oipA (A:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkn
cdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldlls
rvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgdtng
nhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgv
dqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrsc
dlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfp
qlkfkrkveniedfkwedieligyypyptikmdmav

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2oipB (B:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
lqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyld
llsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgd
tngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytg
vgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyq
rscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtpr
pfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >2oipB (B:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkn
cdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldlls
rvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgdtng
nhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgv
dqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrsc
dlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfp
qlkfkrkveniedfkwedieligyypyptikmdmav

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2oipC (C:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
lqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyld
llsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgd
tngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytg
vgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyq
rscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtpr
pfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >2oipC (C:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekcd
pargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrv
lengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgdtngnh
liekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdq
laklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdl
glgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpql
kfkrkveniedfkwedieligyypyptikmdmav

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2oipD (D:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
lqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyld
llsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgd
tngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytg
vgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyq
rscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtpr
pfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >2oipD (D:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkc
dpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsr
vlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgdtngn
hliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvd
qlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscd
lglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpq
lkfkrkveniedfkwedieligyypyptikmdmav

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2oipE (E:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
lqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyld
llsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgd
tngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytg
vgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyq
rscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtpr
pfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records): (download) 
    >2oipE (E:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekkt
gqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlen
gayrenrtgistysifgqmmrfdmresfpllttkkvairgifeeliwfikgdtngnhlie
kkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlak
lietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglg
spfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfk
rkveniedfkwedieligyypyptikmdmav