PDB entry 2j25

View 2j25 on RCSB PDB site
Description: partially deglycosylated glucoceramidase
Class: hydrolase
Keywords: pharmaceutical, gaucher disease, disease mutation, glycosidase, sphingolipid, polymorphism, glycoprotein, membrane, lysosome, hydrolase, glucosidase, glucocerebrosidase, alternative initiation, lipid metabolism, cerezyme hydrolase, sphingolipid metabolism
Deposited on 2006-08-16, released 2006-12-06
The last revision prior to the SCOPe 2.01 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.218
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glucosylceramidase
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04062 (0-496)
      • conflict (494)
    Domains in SCOPe 2.01: d2j25a1, d2j25a2
  • Chain 'B':
    Compound: glucosylceramidase
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04062 (0-496)
      • conflict (494)
    Domains in SCOPe 2.01: d2j25b1, d2j25b2
  • Heterogens: NAG, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2j25A (A:)
    arpcipksfgyssvvcvcnatycdsfdpptfpalgtfsryestrsgrrmelsmgpiqanh
    tgtgllltlqpeqkfqkvkgfggamtdaaalnilalsppaqnlllksyfseegigyniir
    vpmascdfsirtytyadtpddfqlhnfslpeedtklkiplihralqlaqrpvsllaspwt
    sptwlktngavngkgslkgqpgdiyhqtwaryfvkfldayaehklqfwavtaenepsagl
    lsgypfqclgftpehqrdfiardlgptlansthhnvrllmlddqrlllphwakvvltdpe
    aakyvhgiavhwyldflapakatlgethrlfpntmlfaseacvgskfweqsvrlgswdrg
    mqyshsiitnllyhvvgwtdwnlalnpeggpnwvrnfvdspiivditkdtfykqpmfyhl
    ghfskfipegsqrvglvasqkndldavalmhpdgsavvvvlnrsskdvpltikdpavgfl
    etispgysihtylwhrq
    

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2j25B (B:)
    arpcipksfgyssvvcvcnatycdsfdpptfpalgtfsryestrsgrrmelsmgpiqanh
    tgtgllltlqpeqkfqkvkgfggamtdaaalnilalsppaqnlllksyfseegigyniir
    vpmascdfsirtytyadtpddfqlhnfslpeedtklkiplihralqlaqrpvsllaspwt
    sptwlktngavngkgslkgqpgdiyhqtwaryfvkfldayaehklqfwavtaenepsagl
    lsgypfqclgftpehqrdfiardlgptlansthhnvrllmlddqrlllphwakvvltdpe
    aakyvhgiavhwyldflapakatlgethrlfpntmlfaseacvgskfweqsvrlgswdrg
    mqyshsiitnllyhvvgwtdwnlalnpeggpnwvrnfvdspiivditkdtfykqpmfyhl
    ghfskfipegsqrvglvasqkndldavalmhpdgsavvvvlnrsskdvpltikdpavgfl
    etispgysihtylwhrq