PDB entry 2flo

View 2flo on RCSB PDB site
Description: Crystal structure of exopolyphosphatase (PPX) from E. coli O157:H7
Class: hydrolase
Keywords: exopolyphosphatase, PPX/GPPA, metaphosphatase, Structural Genomics, Bacterial Structural Genomics, Montreal-Kingston Bacterial Structural Genomics Initiative, BSGI
Deposited on 2006-01-06, released 2006-06-06
The last revision prior to the SCOP 1.75 freeze date was dated 2006-07-04, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.205
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: exopolyphosphatase
    Species: Escherichia coli
    Gene: ppx
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AFL8
      • modified residue (35)
      • modified residue (45)
      • modified residue (66)
      • modified residue (72)
      • modified residue (137)
      • modified residue (177)
      • modified residue (202)
      • modified residue (224)
      • modified residue (238)
      • modified residue (240)
      • modified residue (315)
      • modified residue (351)
      • modified residue (353)
      • modified residue (378)
      • modified residue (414-415)
    Domains in SCOP 1.75: d2floa1, d2floa2, d2floa3
  • Chain 'B':
    Compound: exopolyphosphatase
    Species: Escherichia coli
    Gene: ppx
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AFL8
      • modified residue (35)
      • modified residue (45)
      • modified residue (66)
      • modified residue (72)
      • modified residue (137)
      • modified residue (177)
      • modified residue (202)
      • modified residue (224)
      • modified residue (238)
      • modified residue (240)
      • modified residue (315)
      • modified residue (351)
      • modified residue (353)
      • modified residue (378)
      • modified residue (414-415)
    Domains in SCOP 1.75: d2flob1, d2flob2, d2flob3
  • Chain 'C':
    Compound: exopolyphosphatase
    Species: Escherichia coli
    Gene: ppx
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AFL8
      • modified residue (35)
      • modified residue (45)
      • modified residue (66)
      • modified residue (72)
      • modified residue (137)
      • modified residue (177)
      • modified residue (202)
      • modified residue (224)
      • modified residue (238)
      • modified residue (240)
      • modified residue (315)
      • modified residue (351)
      • modified residue (353)
      • modified residue (378)
      • modified residue (414-415)
    Domains in SCOP 1.75: d2floc1, d2floc2, d2floc3
  • Chain 'D':
    Compound: exopolyphosphatase
    Species: Escherichia coli
    Gene: ppx
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AFL8
      • modified residue (35)
      • modified residue (45)
      • modified residue (66)
      • modified residue (72)
      • modified residue (137)
      • modified residue (177)
      • modified residue (202)
      • modified residue (224)
      • modified residue (238)
      • modified residue (240)
      • modified residue (315)
      • modified residue (351)
      • modified residue (353)
      • modified residue (378)
      • modified residue (414-415)
    Domains in SCOP 1.75: d2flod1, d2flod2, d2flod3
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2floA (A:)
    mgsshhhhhhgspihdksprpqefaavdlgsnsfhmviarvvdgamqiigrlkqrvhlad
    glgpdnmlseeamtrglnclslfaerlqgfspasvcivgthtlrqalnatdflkraekvi
    pypieiisgneearlifmgvehtqpekgrklvidigggstelvigenfepilvesrrmgc
    vsfaqlyfpggvinkenfqrarmaaaqkletltwqfriqgwnvamgasgtikaahevlme
    mgekdgiitperleklvkevlrhrnfaslslpglseerktvfvpglailcgvfdalaire
    lrlsdgalregvlyemegrfrhqdvrsrtasslanqyhidseqarrvldttmqmyeqwre
    qqpklahpqleallrwaamlhevglninhsglhrhsayilqnsdlpgfnqeqqlmmatlv
    ryhrkaiklddlprftlfkkkqflpliqllrlgvllnnqrqatttpptltlitddshwtl
    rfphdwfsqnalvlldlekeqeywegvagwrlkieeestpeiaa
    

    Sequence, based on observed residues (ATOM records): (download)
    >2floA (A:)
    qefaavdlgsnsfhmviarvvdgamqiigrlkqrvhladglgpdnmlseeamtrglncls
    lfaerlqgfspasvcivgthtlrqalnatdflkraekvipypieiisgneearlifmgve
    htqpekgrklvidigggstelvigenfepilvesrrmgcvsfaqlyfpggvinkenfqra
    rmaaaqkletltwqfriqgwnvamgasgtikaahevlmemgekdgiitperleklvkevl
    rhrnfaslslpglseerktvfvpglailcgvfdalairelrlsdgalregvlyemegrfr
    hqdvrsrtasslanqyhidseqarrvldttmqmyeqwreqqpklahpqleallrwaamlh
    evglninhsglhrhsayilqnsdlpgfnqeqqlmmatlvryhrkaiklddlprftlfkkk
    qflpliqllrlgvllnnqrqatttpptltlitddshwtlrfphdwfsqnalvlldlekeq
    eywegvagwrlkieee
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >2floB (B:)
    mgsshhhhhhgspihdksprpqefaavdlgsnsfhmviarvvdgamqiigrlkqrvhlad
    glgpdnmlseeamtrglnclslfaerlqgfspasvcivgthtlrqalnatdflkraekvi
    pypieiisgneearlifmgvehtqpekgrklvidigggstelvigenfepilvesrrmgc
    vsfaqlyfpggvinkenfqrarmaaaqkletltwqfriqgwnvamgasgtikaahevlme
    mgekdgiitperleklvkevlrhrnfaslslpglseerktvfvpglailcgvfdalaire
    lrlsdgalregvlyemegrfrhqdvrsrtasslanqyhidseqarrvldttmqmyeqwre
    qqpklahpqleallrwaamlhevglninhsglhrhsayilqnsdlpgfnqeqqlmmatlv
    ryhrkaiklddlprftlfkkkqflpliqllrlgvllnnqrqatttpptltlitddshwtl
    rfphdwfsqnalvlldlekeqeywegvagwrlkieeestpeiaa
    

    Sequence, based on observed residues (ATOM records): (download)
    >2floB (B:)
    efaavdlgsnsfhmviarvvdgamqiigrlkqrvhladglgpdnmlseeamtrglnclsl
    faerlqgfspasvcivgthtlrqalnatdflkraekvipypieiisgneearlifmgveh
    tqpekgrklvidigggstelvigenfepilvesrrmgcvsfaqlyfpggvinkenfqrar
    maaaqkletltwqfriqgwnvamgasgtikaahevlmemgekdgiitperleklvkevlr
    hrnfaslslpglseerktvfvpglailcgvfdalairelrlsdgalregvlyemegrfrh
    qdvrsrtasslanqyhidseqarrvldttmqmyeqwreqqpklahpqleallrwaamlhe
    vglninhsglhrhsayilqnsdlpgfnqeqqlmmatlvryhrkaiklddlprftlfkkkq
    flpliqllrlgvllnnqrqatttpptltlitddshwtlrfphdwfsqnalvlldlekeqe
    ywegvagwrlkieee
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >2floC (C:)
    mgsshhhhhhgspihdksprpqefaavdlgsnsfhmviarvvdgamqiigrlkqrvhlad
    glgpdnmlseeamtrglnclslfaerlqgfspasvcivgthtlrqalnatdflkraekvi
    pypieiisgneearlifmgvehtqpekgrklvidigggstelvigenfepilvesrrmgc
    vsfaqlyfpggvinkenfqrarmaaaqkletltwqfriqgwnvamgasgtikaahevlme
    mgekdgiitperleklvkevlrhrnfaslslpglseerktvfvpglailcgvfdalaire
    lrlsdgalregvlyemegrfrhqdvrsrtasslanqyhidseqarrvldttmqmyeqwre
    qqpklahpqleallrwaamlhevglninhsglhrhsayilqnsdlpgfnqeqqlmmatlv
    ryhrkaiklddlprftlfkkkqflpliqllrlgvllnnqrqatttpptltlitddshwtl
    rfphdwfsqnalvlldlekeqeywegvagwrlkieeestpeiaa
    

    Sequence, based on observed residues (ATOM records): (download)
    >2floC (C:)
    qefaavdlgsnsfhmviarvvdgamqiigrlkqrvhladglgpdnmlseeamtrglncls
    lfaerlqgfspasvcivgthtlrqalnatdflkraekvipypieiisgneearlifmgve
    htqpekgrklvidigggstelvigenfepilvesrrmgcvsfaqlyfpggvinkenfqra
    rmaaaqkletltwqfriqgwnvamgasgtikaahevlmemgekdgiitperleklvkevl
    rhrnfaslslpglseerktvfvpglailcgvfdalairelrlsdgalregvlyemegrfr
    hqdvrsrtasslanqyhidseqarrvldttmqmyeqwreqqpklahpqleallrwaamlh
    evglninhsglhrhsayilqnsdlpgfnqeqqlmmatlvryhrkaiklddlprftlfkkk
    qflpliqllrlgvllnnqrqatttpptltlitddshwtlrfphdwfsqnalvlldlekeq
    eywegvagwrlkieee
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >2floD (D:)
    mgsshhhhhhgspihdksprpqefaavdlgsnsfhmviarvvdgamqiigrlkqrvhlad
    glgpdnmlseeamtrglnclslfaerlqgfspasvcivgthtlrqalnatdflkraekvi
    pypieiisgneearlifmgvehtqpekgrklvidigggstelvigenfepilvesrrmgc
    vsfaqlyfpggvinkenfqrarmaaaqkletltwqfriqgwnvamgasgtikaahevlme
    mgekdgiitperleklvkevlrhrnfaslslpglseerktvfvpglailcgvfdalaire
    lrlsdgalregvlyemegrfrhqdvrsrtasslanqyhidseqarrvldttmqmyeqwre
    qqpklahpqleallrwaamlhevglninhsglhrhsayilqnsdlpgfnqeqqlmmatlv
    ryhrkaiklddlprftlfkkkqflpliqllrlgvllnnqrqatttpptltlitddshwtl
    rfphdwfsqnalvlldlekeqeywegvagwrlkieeestpeiaa
    

    Sequence, based on observed residues (ATOM records): (download)
    >2floD (D:)
    qefaavdlgsnsfhmviarvvdgamqiigrlkqrvhladglgpdnmlseeamtrglncls
    lfaerlqgfspasvcivgthtlrqalnatdflkraekvipypieiisgneearlifmgve
    htqpekgrklvidigggstelvigenfepilvesrrmgcvsfaqlyfpggvinkenfqra
    rmaaaqkletltwqfriqgwnvamgasgtikaahevlmemgekdgiitperleklvkevl
    rhrnfaslslpglseerktvfvpglailcgvfdalairelrlsdgalregvlyemegrfr
    hqdvrsrtasslanqyhidseqarrvldttmqmyeqwreqqpklahpqleallrwaamlh
    evglninhsglhrhsayilqnsdlpgfnqeqqlmmatlvryhrkaiklddlprftlfkkk
    qflpliqllrlgvllnnqrqatttpptltlitddshwtlrfphdwfsqnalvlldlekeq
    eywegvagwrlkieee