PDB entry 2f8n

View 2f8n on RCSB PDB site
Description: 2.9 Angstrom X-ray structure of hybrid macroH2A nucleosomes
Class: structural protein/DNA
Keywords: Nucleosome, NCP, macroH2A, Histone variant, chromatin, X-ray structure, crystallography, STRUCTURAL PROTEIN/DNA COMPLEX
Deposited on 2005-12-02, released 2006-05-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.217
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Histone H3.1
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8na_
  • Chain 'B':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8nb_
  • Chain 'D':
    Compound: Histone 3, H2ba
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8nd_
  • Chain 'E':
    Compound: Histone H3.1
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8ne_
  • Chain 'F':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8nf_
  • Chain 'G':
    Compound: Core histone macro-H2A.1
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot O75367
      • conflict (64)
    Domains in SCOPe 2.08: d2f8ng_
  • Chain 'H':
    Compound: Histone H2B.1
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8nh_
  • Chain 'I':
    Compound: alpha-satellite DNA (146 bp)
    Species: Homo sapiens [TaxId:9606]
  • Chain 'J':
    Compound: alpha-satellite DNA (146 bp)
    Species: Homo sapiens [TaxId:9606]
  • Chain 'K':
    Compound: Histone H2A type 1
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f8nk1
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2f8nA (A:)
martkqtarkstggkaprkqlatkaarksapatggvkkphryrpgtvalreirryqkste
llirklpfqrlvreiaqdfktdlrfqssavmalqeaseaylvglfedtnlcaihakrvti
mpkdiqlarrirgera

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nA (A:)
phryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavmalqease
aylvglfedtnlcaihakrvtimpkdiqlarrirgera

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2f8nB (B:)
msgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlk
vflenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nB (B:)
dniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavtytehakrktvta
mdvvyalkrqgrtlygfgg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2f8nD (D:)
mpepsrstpapkkgskkaitkaqkkdgkkrkrgrkesysiyvykvlkqvhpdtgisskam
gimnsfvndiferiaseasrlahynkrstitsrevqtavrlllpgelakhavsegtkavt
kytssk

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nD (D:)
rkesysiyvykvlkqvhpdtgisskamgimnsfvndiferiaseasrlahynkrstitsr
evqtavrlllpgelakhavsegtkavtkytssk

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2f8nE (E:)
martkqtarkstggkaprkqlatkaarksapatggvkkphryrpgtvalreirryqkste
llirklpfqrlvreiaqdfktdlrfqssavmalqeaseaylvglfedtnlcaihakrvti
mpkdiqlarrirgera

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nE (E:)
phryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavmalqease
aylvglfedtnlcaihakrvtimpkdiqlarrirgera

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2f8nF (F:)
msgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlk
vflenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nF (F:)
rkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavtytehakr
ktvtamdvvyalkrqgrtlygfgg

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >2f8nG (G:)
mssrggkkkstktsrsakagvifpvgrmlryikkghpkyrigvgapvymaavleyltaei
lelavnaardnkkgrvtprhillavandeelnqllkgvtiasggvlpnihpellakkrgs

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nG (G:)
stktsrsakagvifpvgrmlryikkghpkyrigvgapvymaavleyltaeilelavnaar
dnkkgrvtprhillavandeelnqllkgvtiasggvlpnihpellakk

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >2f8nH (H:)
maksapapkkgskkavtktqkkdgkkrrktrkesyaiyvykvlkqvhpdtgisskamsim
nsfvndvferiageasrlahynkrstitsreiqtavrlllpgelakhavsegtkavtkyt
sak

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nH (H:)
rkesyaiyvykvlkqvhpdtgisskamsimnsfvndvferiageasrlahynkrstitsr
eiqtavrlllpgelakhavsegtkavtkytsak

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >2f8nK (K:)
mgsshhhhhhssglvprgsmsgrgkqggkarakaktrssraglqfpvgrvhrllrkgnys
ervgagapvylaavleyltaeilelagnaardnkktriiprhlqlairndeelnkllgrv
tiaqggvlpniqavllpkkteshhkakgk

    Sequence, based on observed residues (ATOM records): (download) 
    >2f8nK (K:)
aktrssraglqfpvgrvhrllrkgnyservgagapvylaavleyltaeilelagnaardn
kktriiprhlqlairndeelnkllgrvtiaqggvlpniqavllpk