PDB entry 2f54
View 2f54 on RCSB PDB site
Description: Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity
Class: immune system
Keywords: T-cell receptor, CDR2, Phage display, wild type sequence, high affinity, NY-ESO-1, IMMUNE SYSTEM
Deposited on
2005-11-25, released
2006-04-25
The last revision prior to the SCOPe 2.01 freeze date was dated
2011-07-13, with a file datestamp of
2011-05-08.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.225
AEROSPACI score: 0.28
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: hla class I histocompatibility antigen
Species: Homo sapiens [TaxId:9606]
Gene: HLA-A, HLAA
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54a1, d2f54a2 - Chain 'B':
Compound: Beta-2-microglobulin
Species: Homo sapiens [TaxId:9606]
Gene: B2M
Database cross-references and differences (RAF-indexed):
- Uniprot P61769 (1-99)
- cloning artifact (0)
- engineered (67)
- engineered (91)
Domains in SCOPe 2.01: d2f54b_ - Chain 'C':
Compound: Cancer/testis antigen 1B
Species: synthetic, synthetic
Database cross-references and differences (RAF-indexed):
- Chain 'D':
Compound: T-cell receptor alpha chain
Species: Homo sapiens [TaxId:9606]
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54d1, d2f54d2 - Chain 'E':
Compound: T-cell receptor beta chain
Species: Homo sapiens [TaxId:9606]
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54e1, d2f54e2 - Chain 'F':
Compound: hla class I histocompatibility antigen
Species: Homo sapiens [TaxId:9606]
Gene: HLA-A, HLAA
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54f1, d2f54f2 - Chain 'G':
Compound: Beta-2-microglobulin
Species: Homo sapiens [TaxId:9606]
Gene: B2M
Database cross-references and differences (RAF-indexed):
- Uniprot P61769 (1-99)
- cloning artifact (0)
- engineered (67)
- engineered (91)
Domains in SCOPe 2.01: d2f54g_ - Chain 'H':
Compound: Cancer/testis antigen 1B
Species: synthetic, synthetic
Database cross-references and differences (RAF-indexed):
- Chain 'K':
Compound: T-cell receptor alpha chain
Species: Homo sapiens [TaxId:9606]
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54k1, d2f54k2 - Chain 'L':
Compound: T-cell receptor beta chain
Species: Homo sapiens [TaxId:9606]
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.01: d2f54l1, d2f54l2 - Heterogens: HOH
PDB Chain Sequences:
- Chain 'A':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54A (A:)
gshsmryfftsvsrpgrgeprfiavgyvddtqfvrfdsdaasqrmeprapwieqegpeyw
dgetrkvkahsqthrvdlgtlrgyynqseagshtvqrmygcdvgsdwrflrgyhqyaydg
kdyialkedlrswtaadmaaqttkhkweaahvaeqlraylegtcvewlrrylengketlq
rtdapkthmthhavsdheatlrcwalsfypaeitltwqrdgedqtqdtelvetrpagdgt
fqkwaavvvpsgqeqrytchvqheglpkpltlrw
- Chain 'B':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54B (B:)
miqrtpkiqvysrhpaengksnflncyvsgfhpsdievdllkngeriekvehsdlsfskd
wsfyllycteftptekdeyacrvnhvtlsqpcivkwdrdm
- Chain 'C':
No sequence available.
- Chain 'D':
Sequence, based on SEQRES records: (download)
>2f54D (D:)
kqqvtqipaalsvpegenlvlncsftdsaiynlqwfrqdpggkltsllliqssqreqtsg
rlnasldksagsstlyiaasqpgdsatylcavrptsggsyiptfgrgtslivhpyiqnpd
pavyqlrdskssdksvclftdfdsqtnvsqskdsdvyitdkcvldmrsmdfksnsavaws
nksdfacanafnnsiipedtffpspe
Sequence, based on observed residues (ATOM records): (download)
>2f54D (D:)
qqvtqipaalsvpegenlvlncsftdsaiynlqwfrqdpggkltsllliqssqreqtsgr
lnasldksagsstlyiaasqpgdsatylcavrptsggsyiptfgrgtslivhpyiqnpdp
avyqlrdskssdksvclftdfdsqtnvsqskdsdvyitdkcvldmrsmdfksnsavawsn
ksdfacanafnnsiipedtffpspe
- Chain 'E':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54E (E:)
gvtqtpkfqvlktgqsmtlqcaqdmnheymswyrqdpgmglrlihysvgagitdqgevpn
gynvsrsttedfplrllsaapsqtsvyfcassyvgntgelffgegsrltvledlknvfpp
evavfepseaeishtqkatlvclatgfypdhvelswwvngkevhsgvctdpqplkeqpal
ndsryalssrlrvsatfwqdprnhfrcqvqfyglsendewtqdrakpvtqivsaeawgra
d
- Chain 'F':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54F (F:)
gshsmryfftsvsrpgrgeprfiavgyvddtqfvrfdsdaasqrmeprapwieqegpeyw
dgetrkvkahsqthrvdlgtlrgyynqseagshtvqrmygcdvgsdwrflrgyhqyaydg
kdyialkedlrswtaadmaaqttkhkweaahvaeqlraylegtcvewlrrylengketlq
rtdapkthmthhavsdheatlrcwalsfypaeitltwqrdgedqtqdtelvetrpagdgt
fqkwaavvvpsgqeqrytchvqheglpkpltlrw
- Chain 'G':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54G (G:)
miqrtpkiqvysrhpaengksnflncyvsgfhpsdievdllkngeriekvehsdlsfskd
wsfyllycteftptekdeyacrvnhvtlsqpcivkwdrdm
- Chain 'H':
No sequence available.
- Chain 'K':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54K (K:)
kqqvtqipaalsvpegenlvlncsftdsaiynlqwfrqdpggkltsllliqssqreqtsg
rlnasldksagsstlyiaasqpgdsatylcavrptsggsyiptfgrgtslivhpyiqnpd
pavyqlrdskssdksvclftdfdsqtnvsqskdsdvyitdkcvldmrsmdfksnsavaws
nksdfacanafnnsiipedtffpspe
- Chain 'L':
Sequence; same for both SEQRES and ATOM records: (download)
>2f54L (L:)
gvtqtpkfqvlktgqsmtlqcaqdmnheymswyrqdpgmglrlihysvgagitdqgevpn
gynvsrsttedfplrllsaapsqtsvyfcassyvgntgelffgegsrltvledlknvfpp
evavfepseaeishtqkatlvclatgfypdhvelswwvngkevhsgvctdpqplkeqpal
ndsryalssrlrvsatfwqdprnhfrcqvqfyglsendewtqdrakpvtqivsaeawgra
d