PDB entry 2d33

View 2d33 on RCSB PDB site
Description: Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride
Class: Ligase
Keywords: glutathione homeostasis, peptide synthesis, phosphoryl transfer, Ligase
Deposited on 2005-09-25, released 2006-11-14
The last revision prior to the SCOPe 2.02 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: 0.164
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Glutamate--cysteine ligase
    Species: Escherichia coli [TaxId:562]
    Gene: gshI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6W9 (0-517)
      • engineered (105)
      • engineered (204)
      • engineered (222)
    Domains in SCOPe 2.02: d2d33a_
  • Chain 'B':
    Compound: Glutamate--cysteine ligase
    Species: Escherichia coli [TaxId:562]
    Gene: gshI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6W9 (0-517)
      • engineered (105)
      • engineered (163)
      • engineered (204)
      • engineered (222)
    Domains in SCOPe 2.02: d2d33b_
  • Chain 'C':
    Compound: Glutamate--cysteine ligase
    Species: Escherichia coli [TaxId:562]
    Gene: gshI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6W9 (0-517)
      • engineered (105)
      • engineered (204)
    Domains in SCOPe 2.02: d2d33c_
  • Chain 'D':
    Compound: Glutamate--cysteine ligase
    Species: Escherichia coli [TaxId:562]
    Gene: gshI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6W9 (0-End)
      • engineered (105)
      • engineered (204)
      • engineered (222)
    Domains in SCOPe 2.02: d2d33d_
  • Heterogens: MG, GLU, CYS, ADP, AF3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2d33A (A:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

    Sequence, based on observed residues (ATOM records): (download) 
    >2d33A (A:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgadakekisagyfrvi
rnyyrfgwvipylfgaspaisssfltslpfektesgmyylpyatslrlsdlgytnksqsn
lgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrv
trsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemsss
elactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggea
yqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedf
vaereaserrqqemeaadtepfavwlekha

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2d33B (B:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

    Sequence, based on observed residues (ATOM records): (download) 
    >2d33B (B:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksakekisagyfrvirny
yrfgwvipylfgaspaisssflslpfektesgmyylpyatslrlsdlgytnsnlgitfnd
lyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrsgesp
sdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactrv
nwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcde
lvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaereas
errqqemeaadtepfavwlekha

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2d33C (C:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

    Sequence, based on observed residues (ATOM records): (download) 
    >2d33C (C:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqakisgadakekisagyfrv
irnyyrfgwvipylfgaspaisssflslpfektmyylpyatslrlsdlgytnsnlgitfn
dlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrsges
psdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactr
vnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcd
elvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaerea
serrqqemeaadtepfavwlekha

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2d33D (D:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqaksgdisgadakekisagy
frvirnyyrfgwvipylfgaspaisssflqgkptslpfektesgmyylpyatslrlsdlg
ytnksqsnlgitfndlyeyvaglkqaiktpseeyakigiekdgkrlqinsnvlqienely
apirpkrvtrsgespsdallrggieyievrsldinpfspigvdeqqvrfldlfmvwcala
dapemssselactrvnwnrvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtl
dsinggeayqkvcdelvacfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepl
eilreedfvaereaserrqqemeaadtepfavwlekha

    Sequence, based on observed residues (ATOM records): (download) 
    >2d33D (D:)
mipdvsqalawlekhpqalkgiqrgleretlrvnadgtlattghpealgsalthkwittd
faeallefitpvdgdiehmltfmrdlhrytarnmgdermwplsmpsyiaegqdielaqyg
tsntgrfktlyreglknrygalmqtisgvhynfslpmafwqakekisagyfrvirnyyrf
gwvipylfgaspaisssflslpfektesgmyylpyatslrlsdlgytnsnlgitfndlye
yvaglkqaiktpseeyakigiekdgkrlqinsnvlqienelyapirpkrvtrsgespsda
llrggieyievrsldinpfspigvdeqqvrfldlfmvwcaladapemssselactrvnwn
rvilegrkpgltlgigcetaqfplpqvgkdlfrdlkrvaqtldsinggeayqkvcdelva
cfdnpdltfsarilrsmidtgiggtgkafaeayrnllreepleilreedfvaereaserr
qqemeaadtepfavwlekh