PDB entry 1tth

View 1tth on RCSB PDB site
Description: Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA)
Class: hydrolase/hydrolase regulator
Keywords: site-specific mutagenesis, domain closure, allosteric transition
Deposited on 2004-06-22, released 2004-07-20
The last revision prior to the SCOP 1.73 freeze date was dated 2004-08-17, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.172
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aspartate carbamoyltransferase catalytic chain
    Species: Escherichia coli
    Gene: PYRB, B4245, C5345, Z5856, ECS5222, SF4245, S4507
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A786 (0-309)
      • engineered (49)
    Domains in SCOP 1.73: d1ttha2, d1ttha3
  • Chain 'B':
    Compound: Aspartate carbamoyltransferase regulatory chain
    Species: Escherichia coli
    Gene: PYRI, B4244, C5344, Z5855, ECS5221
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7F3 (1-152)
      • initiating methionine (0)
    Domains in SCOP 1.73: d1tthb1, d1tthb2
  • Chain 'C':
    Compound: Aspartate carbamoyltransferase catalytic chain
    Species: Escherichia coli
    Gene: PYRB, B4245, C5345, Z5856, ECS5222, SF4245, S4507
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A786 (0-309)
      • engineered (49)
    Domains in SCOP 1.73: d1tthc2, d1tthc3
  • Chain 'D':
    Compound: Aspartate carbamoyltransferase regulatory chain
    Species: Escherichia coli
    Gene: PYRI, B4244, C5344, Z5855, ECS5221
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7F3 (1-152)
      • initiating methionine (0)
    Domains in SCOP 1.73: d1tthd1, d1tthd2
  • Heterogens: ZN, PAL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tthA (A:)
anplyqkhiisindlsrddlnlvlataaklkanpqpellkhkviascffaastrtrlsfe
tsmhrlgasvvgfsdsantslgkkgetladtisvistyvdaivmrhpqegaarlatefsg
nvpvlnagdgsnqhptqtlldlftiqetqgrldnlhvamvgdlkygrtvhsltqalakfd
gnrfyfiapdalampqyildmldekgiawslhssieevmaevdilymtrvqkerldpsey
anvkaqfvlrasdlhnakanmkvlhplprvdeiatdvdktphawyfqqagngifarqall
alvlnrdlvl

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tthB (B:)
mthdnklqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlik
ientflsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaep
vsssfavrkrandialkckycekefshnvvlan

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tthC (C:)
anplyqkhiisindlsrddlnlvlataaklkanpqpellkhkviascffaastrtrlsfe
tsmhrlgasvvgfsdsantslgkkgetladtisvistyvdaivmrhpqegaarlatefsg
nvpvlnagdgsnqhptqtlldlftiqetqgrldnlhvamvgdlkygrtvhsltqalakfd
gnrfyfiapdalampqyildmldekgiawslhssieevmaevdilymtrvqkerldpsey
anvkaqfvlrasdlhnakanmkvlhplprvdeiatdvdktphawyfqqagngifarqall
alvlnrdlvl

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1tthD (D:)
mthdnklqveaikrgtvidhipaqigfkllslfkltetdqritiglnlpsgemgrkdlik
ientflsedqvdqlalyapqatvnridnyevvgksrpslperidnvlvcpnsncishaep
vsssfavrkrandialkckycekefshnvvlan