PDB entry 1t97

View 1t97 on RCSB PDB site
Description: Use of sequence duplication to engineer a ligand-triggered long-distance molecular switch in T4 Lysozyme
Class: hydrolase
Keywords: molecular switch, t4 lysozyme, nano-biotechnology, protein engineering, protein design, sequence duplication, hydrolase
Deposited on 2004-05-14, released 2004-08-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-12-19, with a file datestamp of 2018-12-14.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.17 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lysozyme
    Species: Enterobacteria phage T4 sensu lato [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (0-End)
      • engineered (38)
      • insertion (39-49)
      • engineered (62)
      • engineered (64)
      • engineered (107)
    Domains in SCOPe 2.08: d1t97a_
  • Chain 'B':
    Compound: lysozyme
    Species: Enterobacteria phage T4 sensu lato [TaxId:10665]
    Gene: E
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00720 (0-End)
      • engineered (38)
      • insertion (39-49)
      • engineered (62)
      • engineered (64)
      • engineered (107)
    Domains in SCOPe 2.08: d1t97b_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1t97A (A:)
mnifemlrideglrlkiykdtegyytigighlltkspsinaakseldkainaakseldka
igantngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrraalinmvfqmget
gvagftnslrmlqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >1t97A (A:)
mnifemlrideglrlkiykdtegyytigighlltkspsinaakseldkainaagantngv
itkdeaeklfnqdvdaavrgilrnaklkpvydsldavrraalinmvfqmgetgvagftns
lrmlqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1t97B (B:)
mnifemlrideglrlkiykdtegyytigighlltkspsinaakseldkainaakseldka
igantngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrraalinmvfqmget
gvagftnslrmlqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayknl

    Sequence, based on observed residues (ATOM records): (download) 
    >1t97B (B:)
mnifemlrideglrlkiykdtegyytigighlltkspsinaakseldkainaakseldka
igantngvitkdeaeklfnqdvdaavrgilrnaklkpvydsldavrraalinmvfqmget
gvagftnslrmlqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdayk