PDB entry 1pi3

View 1pi3 on RCSB PDB site
Description: E28Q mutant Benzoylformate Decarboxylase From Pseudomonas Putida
Class: lyase
Keywords: lyase, decarboxylase, mandelate catabolism, thiamin diphosphate, mutant, x-ray diffraction, high resolution
Deposited on 2003-05-29, released 2004-11-23
The last revision prior to the SCOP 1.73 freeze date was dated 2006-09-26, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.2 Å
R-factor: 0.121
AEROSPACI score: 0.86 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Benzoylformate decarboxylase
    Species: Pseudomonas putida
    Gene: MDLC
    Database cross-references and differences (RAF-indexed):
    • Uniprot P20906
      • engineered (27)
    Domains in SCOP 1.73: d1pi3a1, d1pi3a2, d1pi3a3
  • Heterogens: MG, CA, TZD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1pi3A (A:)
masvhgttyellrrqgidtvfgnpgsnqlpflkdfpedfryilalqeacvvgiadgyaqa
srkpafinlhsaagtgnamgalsnawnshsplivtagqqtramigvealltnvdaanlpr
plvkwsyepasaaevphamsraihmasmapqgpvylsvpyddwdkdadpqshhlfdrhvs
ssvrlndqdldilvkalnsasnpaivlgpdvdaananadcvmlaerlkapvwvapsaprc
pfptrhpcfrglmpagiaaisqlleghdvvlvigapvfryhqydpgqylkpgtrlisvtc
dpleaarapmgdaivadigamasalanlveessrqlptaapepakvdqdagrlhpetvfd
tlndmapenaiylneststtaqmwqrlnmrnpgsyyfcaagglgfalpaaigvqlaeper
qviavigdgsanysisalwtaaqyniptifvimnngtygalrwfagvleaenvpgldvpg
idfralakgygvqalkadnleqlkgslqealsakgpvlievstvspvk

    Sequence, based on observed residues (ATOM records): (download) 
    >1pi3A (A:)
asvhgttyellrrqgidtvfgnpgsnqlpflkdfpedfryilalqeacvvgiadgyaqas
rkpafinlhsaagtgnamgalsnawnshsplivtagqqtramigvealltnvdaanlprp
lvkwsyepasaaevphamsraihmasmapqgpvylsvpyddwdkdadpqshhlfdrhvss
svrlndqdldilvkalnsasnpaivlgpdvdaananadcvmlaerlkapvwvapsaprcp
fptrhpcfrglmpagiaaisqlleghdvvlvigapvfryhqydpgqylkpgtrlisvtcd
pleaarapmgdaivadigamasalanlveessrqlptaapepakvdqdagrlhpetvfdt
lndmapenaiylneststtaqmwqrlnmrnpgsyyfcaagglgfalpaaigvqlaeperq
viavigdgsanysisalwtaaqyniptifvimnngtygalrwfagvleaenvpgldvpgi
dfralakgygvqalkadnleqlkgslqealsakgpvlievstv