PDB entry 1p86

View 1p86 on RCSB PDB site
Description: Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome
Class: ribosome
Keywords: EF-G.GTP-bound state, ratchet-like movement, real-space refinement, RIBOSOME
Deposited on 2003-05-06, released 2003-06-17
The last revision prior to the SCOPe 2.02 freeze date was dated 2012-02-15, with a file datestamp of 2012-02-10.
Experiment type: EM
Resolution: 11.5 Å
R-factor: N/A
AEROSPACI score: -0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 23S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain '1':
    Compound: 50S ribosomal protein L33
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p861_
  • Chain '4':
    Compound: 50S ribosomal protein L36
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p864_
  • Chain '6':
    Compound: tRNA-PHE
    Species: Escherichia coli [TaxId:562]
  • Chain '9':
    Compound: 5S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'A':
    Compound: 50S ribosomal protein L2
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86a_
  • Chain 'B':
    Compound: 50S ribosomal protein L3
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86b_
  • Chain 'C':
    Compound: 50S ribosomal protein L4
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86c_
  • Chain 'D':
    Compound: 50S ribosomal protein L5
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86d_
  • Chain 'E':
    Compound: 50S ribosomal protein L6
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86e_
  • Chain 'F':
    Compound: 50S ribosomal protein L9
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86f_
  • Chain 'G':
    Compound: 50S ribosomal protein L11
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86g_
  • Chain 'H':
    Compound: 50S ribosomal protein L13
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86h_
  • Chain 'I':
    Compound: 50S ribosomal protein L14
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86i_
  • Chain 'J':
    Compound: 50S ribosomal protein L15
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86j_
  • Chain 'K':
    Compound: 50S ribosomal protein L16
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86k_
  • Chain 'L':
    Compound: 50S ribosomal protein L17
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86l_
  • Chain 'M':
    Compound: 50S ribosomal protein L18
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86m_
  • Chain 'N':
    Compound: 50S ribosomal protein L19
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86n_
  • Chain 'O':
    Compound: 50S ribosomal protein L20
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86o_
  • Chain 'Q':
    Compound: 50S ribosomal protein L22
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86q_
  • Chain 'R':
    Compound: 50S ribosomal protein L23
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86r_
  • Chain 'S':
    Compound: 50S ribosomal protein L24
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86s_
  • Chain 'T':
    Compound: 50S ribosomal protein L25
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86t_
  • Chain 'U':
    Compound: 50S ribosomal protein L27
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86u_
  • Chain 'W':
    Compound: 50S ribosomal protein L29
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86w_
  • Chain 'X':
    Compound: 50S ribosomal protein L30
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86x_
  • Chain 'Z':
    Compound: 50S ribosomal protein L32
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d1p86z_

PDB Chain Sequences:

  • Chain '0':
    No sequence available.

  • Chain '1':
    Sequence, based on SEQRES records: (download) 
    >1p861 (1:)
akgirekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeakik

    Sequence, based on observed residues (ATOM records): (download) 
    >1p861 (1:)
akgirekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeak

  • Chain '4':
    Sequence, based on SEQRES records: (download) 
    >1p864 (4:)
mkvrasvkklcrnckivkrdgvirvicsaepkhkqrqg

    Sequence, based on observed residues (ATOM records): (download) 
    >1p864 (4:)
kvrasvkklcrnckivkrdgvirvicsaepkhkqrq

  • Chain '6':
    No sequence available.

  • Chain '9':
    No sequence available.

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1p86A (A:)
avvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkqa
yrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgvd
aaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsgem
rkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnfg
khpvtpwgvqtkgkktrsnkrtdkfivrrrsk

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86A (A:)
ksggrnnngrittrhiggghkqayrivdfkrnkdgipavverleydpnrsanialvlykd
gerryilapkglkagdqiqsgvdaaikpgntlpmrnipvgstvhnvemkpgkggqlarsa
gtyvqivardgayvtlrlrsgemrkveadcratlgevgnaehmlrvlgkagaarwrgvrp
tvrgtamnpvdhphgggegrnfgkhpvtpwgvqtkgkktrsnkrtdk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1p86B (B:)
miglvgkkvgmtriftedgvsipvtvieveanrvtqvkdlandgyraiqvttgakkanrv
tkpeaghfakagveagrglwefrlaegeeftvgqsisvelfadvkkvdvtgtskgkgfag
tvkrwnfrtqdathgnslshrvpgsigqnqtpgkvfkgkkmagqmgnervtvqsldvvrv
daernlllvkgavpgatgsdlivkpavka

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86B (B:)
miglvgkkvgmtriftedgvsipvtvieveanrvtqvkdlandgyraiqvttgakkanrv
tkpeaghfakagveagrglwefrlaegeeftvgqsisvelfadvkkvdvtgtskervtvq
sldvvrvdaernlllvkgavpgatgsdlivkpavka

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1p86C (C:)
melvlkdaqsaltvsettfgrdfnealvhqvvvayaagarqgtraqktraevtgsgkkpw
rqkgtgrarsgsikspiwrsggvtfaarpqdhsqkvnkkmyrgalksilselvrqdrliv
vekfsveapktkllaqklkdmaledvliitgeldenlflaarnlhkvdvrdatgidpvsl
iafdkvvmtadavkqveemla

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86C (C:)
melvlkdaqsaltvsettfgrdfnealvhqvvvayaagarnkkmyrgalksilselvrqd
rlivvekfsveapktkllaqklkdmaledvliitgeldenlflaarnlhkvdvrdatgid
pvsliafdkvvmtadavkqvee

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1p86D (D:)
aklhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaais
gqkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsaks
fdgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86D (D:)
klhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaaisg
qkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsaksf
dgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >1p86E (E:)
srvakapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyadg
waqagtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpagi
taecptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtkeakkk

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86E (E:)
kapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyadgwaqa
gtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpagitaec
ptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtk

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1p86F (F:)
mqvilldkvanlgslgdqvnvkagyarnflvpqgkavpatkknieffearraeleaklae
vlaaanaraekinaletvtiaskagdegklfgsigtrdiadavtaagvevaksevrlpng
vlrttgehevsfqvhsevfakvivnvvae

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >1p86G (G:)
akkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvitv
yadrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtgad
ieamtrsiegtarsmglvved

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86G (G:)
kkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvitvy
adrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtgadi
eamtrsiegtarsmglvve

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1p86H (H:)
mktftakpetvkrdwyvvdatgktlgrlatelarrlrgkhkaeytphvdtgdyiivlnad
kvavtgnkrtdkvyyhhtghiggikqatfeemiarrpervieiavkgmlpkgplgramfr
klkvyagnehnhaaqqpqvldi

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >1p86I (I:)
miqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlka
vvvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislap
evl

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86I (I:)
iqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlkav
vvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislape
vl

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >1p86J (J:)
mrlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrr
lpkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpv
tvrglrvtkgaraaieaaggkiee

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86J (J:)
taeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpvtvrglrvtkgara
aieaaggkie

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >1p86K (K:)
mlqpkrtkfrkmhkgrnrglaqgtdvsfgsfglkavgrgrltarqieaarramtravkrq
gkiwirvfpdkpitekplavrmgkgkgnveywvaliqpgkvlyemdgvpeelareafkla
aaklpikttfvtktvm

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86K (K:)
qpkrtkfrkmhkgrnrglaqgtdvsfgsfglkavgrgrltarqieaarramtravkrqgk
iwirvfpdkpitekplavrmgkgkgnveywvaliqpgkvlyemdgvpeelareafklaaa
klpikttfvtk

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >1p86L (L:)
mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse
kaeaaae

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86L (L:)
qamfrnmagslvrheiikttlpkakelrrvveplitlaktdsvanrrlafartrdneiva
klfnelgprfasraggytrilkcgfragdnapmayielv

  • Chain 'M':
    Sequence, based on SEQRES records: (download) 
    >1p86M (M:)
mdkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiae
qlkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86M (M:)
kksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiaeql
kytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareagl

  • Chain 'N':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1p86N (N:)
sniikqleqeqmkqdvpsfrpgdtvevkvwvvegskkrlqafegvviairnrglhsaftv
rkisngegvervfqthspvvdsisvkrrgavrkaklyylrertgkaarikerln

  • Chain 'O':
    Sequence, based on SEQRES records: (download) 
    >1p86O (O:)
arvkrgviararhkkilkqakgyygarsrvyrvafqavikagqyayrdrrqrkrqfrqlw
iarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftalvekakaala

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86O (O:)
rrqrkrqfrqlwiarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftal
vekakaal

  • Chain 'Q':
    Sequence, based on SEQRES records: (download) 
    >1p86Q (Q:)
metiakhrharssaqkvrlvadlirgkkvsqaldiltytnkkaavlvkkvlesaianaeh
ndgadiddlkvtkifvdegpsmkrimprakgradrilkrtshitvvvsdr

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86Q (Q:)
akhrharssaqkvrlvadlirgkkvsqaldiltytnkkaavlvkkvlesaianaehndga
diddlkvtkifvdegpsmkrimprakgradrilkrtshitvvvsdr

  • Chain 'R':
    Sequence, based on SEQRES records: (download) 
    >1p86R (R:)
mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
lvvkgkvkrhgqrigrrsdwkkayvtlkegqnldfvggae

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86R (R:)
llkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvntlvkkay
vtlkegqnldfvgg

  • Chain 'S':
    Sequence, based on SEQRES records: (download) 
    >1p86S (S:)
aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnsetik

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86S (S:)
kirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivekea
aiqvsnvaifnaatgkadrvgfrfedgkkvrffksnset

  • Chain 'T':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1p86T (T:)
mftinaevrkeqgkgasrrlraankfpaiiyggkeaplaieldhdkvmnmqakaefysev
ltivvdgkeikvkaqdvqrhpykpklqhidfvra

  • Chain 'U':
    Sequence, based on SEQRES records: (download) 
    >1p86U (U:)
ahkkaggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfa
kadgkvkfevkgpknrkfisieae

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86U (U:)
akrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfakadgkvkfevkgpknr
kfisieae

  • Chain 'W':
    Sequence, based on SEQRES records: (download) 
    >1p86W (W:)
mkakelreksveelntellnllreqfnlrmqaasgqlqqshllkqvrrdvarvktllnek
aga

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86W (W:)
mkakelreksveelntellnllreqfnlrmqaasgqlqqshllkqvrrdvarvktllnek

  • Chain 'X':
    Sequence, based on SEQRES records: (download) 
    >1p86X (X:)
aktikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86X (X:)
tikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee

  • Chain 'Z':
    Sequence, based on SEQRES records: (download) 
    >1p86Z (Z:)
avqqnkptrskrgmrrshdaltavtslsvdktsgekhlrhhitadgyyrgrkviak

    Sequence, based on observed residues (ATOM records): (download) 
    >1p86Z (Z:)
lsvdktsgekhlrhhitadgyyrgrkvia