PDB entry 1ms8

View 1ms8 on RCSB PDB site
Description: Triclinic form of Trypanosoma cruzi trans-sialidase, in complex with 3-deoxy-2,3-dehydro-N-acetylneuraminic acid (DANA)
Class: Hydrolase
Keywords: sialidase, trans-glycosylation, protein-carbohydrate interactions, beta-propeller
Deposited on 2002-09-19, released 2003-03-25
The last revision prior to the SCOP 1.73 freeze date was dated 2003-03-25, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.166
AEROSPACI score: 0.47 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Trans-sialidase
    Species: Trypanosoma cruzi
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q26964 (14-End)
      • engineered (71)
      • see remark 999 (275)
      • see remark 999 (489)
      • see remark 999 (497)
      • engineered (508-509)
      • engineered (533)
      • see remark 999 (571)
      • engineered (606)
      • engineered (610)
      • engineered (612)
    Domains in SCOP 1.73: d1ms8a1, d1ms8a2
  • Chain 'B':
    Compound: Trans-sialidase
    Species: Trypanosoma cruzi
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q26964
      • engineered (71)
      • see remark 999 (275)
      • see remark 999 (489)
      • see remark 999 (497)
      • engineered (508-509)
      • engineered (533)
      • see remark 999 (571)
      • engineered (606)
      • engineered (610)
      • engineered (612)
    Domains in SCOP 1.73: d1ms8b1, d1ms8b2
  • Heterogens: DAN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1ms8A (A:)
    mggshhhhhhgmaslapgssrvelfkrqsskvpfekdgkvtervvhsfrlpalvnvdgvm
    vaiadaryetsfdnslidtvakysvddgetwetqiaiknsrassvsrvvdptvivkgnkl
    yvlvgsynssrsywtshgdardwdillavgevtkstaggkitasikwgspvslkeffpae
    megmhtnqflggagvaivasngnlvypvqvtnkkkqvfskifysedegktwkfgkgrsaf
    gcsepvalewegkliintrvdyrrrlvyessdmgntwleavgtlsrvwgpspksnqpgsq
    ssftavtiegmrvmlfthplnfkgrwlrdrlnlwltdnqriynvgqvsigdensayssvl
    ykddklyclheinsnevyslvfarlvgelriiksvlqswknwdshlssictpadpaasss
    ergcgpavttvglvgflshsatktewedayrcvnastanaervpnglkfagvgggalwpv
    sqqgqnqryhfanhaftlvasvtihevpkgaspllgasldssggkkllglsydkrhqwqp
    iygstpvtptgswemgkryhvvltmankigsvyidgeplegsgqtvvpdertpdishfyv
    ggykrsgmptdsrvtvnnvllynrqlnaeeirtlflsqdligteahmd
    

    Sequence, based on observed residues (ATOM records): (download)
    >1ms8A (A:)
    lapgssrvelfkrqsskvpfekdgkvtervvhsfrlpalvnvdgvmvaiadaryetsfdn
    slidtvakysvddgetwetqiaiknsrassvsrvvdptvivkgnklyvlvgsynssrsyw
    tshgdardwdillavgevtkstaggkitasikwgspvslkeffpaemegmhtnqflggag
    vaivasngnlvypvqvtnkkkqvfskifysedegktwkfgkgrsafgcsepvalewegkl
    iintrvdyrrrlvyessdmgntwleavgtlsrvwgpspksnqpgsqssftavtiegmrvm
    lfthplnfkgrwlrdrlnlwltdnqriynvgqvsigdensayssvlykddklyclheins
    nevyslvfarlvgelriiksvlqswknwdshlssictpagcgpavttvglvgflshsatk
    tewedayrcvnastanaervpnglkfagvgggalwpvsqqgqnqryhfanhaftlvasvt
    ihevpkgaspllgasldssggkkllglsydkrhqwqpiygstpvtptgswemgkryhvvl
    tmankigsvyidgeplegsgqtvvpdertpdishfyvggykrsgmptdsrvtvnnvllyn
    rqlnaeeirtlflsqdligteahm
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1ms8B (B:)
    mggshhhhhhgmaslapgssrvelfkrqsskvpfekdgkvtervvhsfrlpalvnvdgvm
    vaiadaryetsfdnslidtvakysvddgetwetqiaiknsrassvsrvvdptvivkgnkl
    yvlvgsynssrsywtshgdardwdillavgevtkstaggkitasikwgspvslkeffpae
    megmhtnqflggagvaivasngnlvypvqvtnkkkqvfskifysedegktwkfgkgrsaf
    gcsepvalewegkliintrvdyrrrlvyessdmgntwleavgtlsrvwgpspksnqpgsq
    ssftavtiegmrvmlfthplnfkgrwlrdrlnlwltdnqriynvgqvsigdensayssvl
    ykddklyclheinsnevyslvfarlvgelriiksvlqswknwdshlssictpadpaasss
    ergcgpavttvglvgflshsatktewedayrcvnastanaervpnglkfagvgggalwpv
    sqqgqnqryhfanhaftlvasvtihevpkgaspllgasldssggkkllglsydkrhqwqp
    iygstpvtptgswemgkryhvvltmankigsvyidgeplegsgqtvvpdertpdishfyv
    ggykrsgmptdsrvtvnnvllynrqlnaeeirtlflsqdligteahmd
    

    Sequence, based on observed residues (ATOM records): (download)
    >1ms8B (B:)
    apgssrvelfkrqsskvpfekdgkvtervvhsfrlpalvnvdgvmvaiadaryetsfdns
    lidtvakysvddgetwetqiaiknsrassvsrvvdptvivkgnklyvlvgsynssrsywt
    shgdardwdillavgevtkstaggkitasikwgspvslkeffpaemegmhtnqflggagv
    aivasngnlvypvqvtnkkkqvfskifysedegktwkfgkgrsafgcsepvalewegkli
    intrvdyrrrlvyessdmgntwleavgtlsrvwgpspksnqpgsqssftavtiegmrvml
    fthplnfkgrwlrdrlnlwltdnqriynvgqvsigdensayssvlykddklyclheinsn
    evyslvfarlvgelriiksvlqswknwdshlssictpagcgpavttvglvgflshsatkt
    ewedayrcvnastanaervpnglkfagvgggalwpvsqqgqnqryhfanhaftlvasvti
    hevpkgaspllgasldssggkkllglsydkrhqwqpiygstpvtptgswemgkryhvvlt
    mankigsvyidgeplegsgqtvvpdertpdishfyvggykrsgmptdsrvtvnnvllynr
    qlnaeeirtlflsqdligteahm