PDB entry 1kl3

View 1kl3 on RCSB PDB site
Description: an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm1-StrepII
Class: peptide binding protein
Keywords: biotin, crystal structure, protein engineering, strep-tag, streptavidin, peptide binding protein
Deposited on 2001-12-11, released 2002-04-03
The last revision prior to the SCOPe 2.02 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.177
AEROSPACI score: 0.55 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (31-32)
      • engineered (34)
    Domains in SCOPe 2.02: d1kl3a_
  • Chain 'B':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (31-32)
      • engineered (34)
    Domains in SCOPe 2.02: d1kl3b_
  • Chain 'C':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (31-32)
      • engineered (34)
    Domains in SCOPe 2.02: d1kl3c_
  • Chain 'D':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered (31-32)
      • engineered (34)
    Domains in SCOPe 2.02: d1kl3d_
  • Chain 'E':
    Compound: strep-tag II peptide
    Species: synthetic, synthetic
    Database cross-references and differences (RAF-indexed):
    • PDB 1KL3
  • Chain 'F':
    Compound: strep-tag II peptide
    Species: synthetic, synthetic
    Database cross-references and differences (RAF-indexed):
    • PDB 1KL3 (Start-8)
  • Chain 'G':
    Compound: strep-tag II peptide
    Species: synthetic, synthetic
    Database cross-references and differences (RAF-indexed):
    • PDB 1KL3 (Start-8)
  • Chain 'H':
    Compound: strep-tag II peptide
    Species: synthetic, synthetic
    Database cross-references and differences (RAF-indexed):
    • PDB 1KL3 (Start-8)
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1kl3A (A:)
meagitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1kl3A (A:)
gitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvkp
s

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1kl3B (B:)
meagitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1kl3B (B:)
agitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgtalg
wtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1kl3C (C:)
meagitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1kl3C (C:)
agitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgtalg
wtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvk

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1kl3D (D:)
meagitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgta
lgwtvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftk
vkpsaas

    Sequence, based on observed residues (ATOM records): (download) 
    >1kl3D (D:)
gitgtwynqlgstfivtagadgaltgtyvtargnaesryvltgrydsapatdgsgtalgw
tvawknnyrnahsattwsgqyvggaearintqwlltsgtteanawkstlvghdtftkvk

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.