PDB entry 1h7x

View 1h7x on RCSB PDB site
Description: Dihydropyrimidine dehydrogenase (DPD) from pig, ternary complex of a mutant enzyme (C671A), NADPH and 5-fluorouracil
Class: electron transfer
Keywords: electron transfer, flavin, iron-sulfur clusters, pyrimidine catabolism, 5-fluorouracil degradation, oxidoreductase
Deposited on 2001-01-19, released 2001-02-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-02-07, with a file datestamp of 2018-02-02.
Experiment type: XRAY
Resolution: 2.01 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: dihydropyrimidine dehydrogenase
    Species: SUS SCROFA [TaxId:9823]
    Gene: DPYD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q28943
      • conflict (59)
      • engineered mutation (670)
    Domains in SCOPe 2.08: d1h7xa1, d1h7xa2, d1h7xa3, d1h7xa4, d1h7xa5
  • Chain 'B':
    Compound: dihydropyrimidine dehydrogenase
    Species: SUS SCROFA [TaxId:9823]
    Gene: DPYD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q28943
      • conflict (59)
      • engineered mutation (670)
    Domains in SCOPe 2.08: d1h7xb1, d1h7xb2, d1h7xb3, d1h7xb4, d1h7xb5
  • Chain 'C':
    Compound: dihydropyrimidine dehydrogenase
    Species: SUS SCROFA [TaxId:9823]
    Gene: DPYD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q28943
      • conflict (59)
      • engineered mutation (670)
    Domains in SCOPe 2.08: d1h7xc1, d1h7xc2, d1h7xc3, d1h7xc4, d1h7xc5
  • Chain 'D':
    Compound: dihydropyrimidine dehydrogenase
    Species: SUS SCROFA [TaxId:9823]
    Gene: DPYD
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q28943
      • conflict (59)
      • engineered mutation (670)
    Domains in SCOPe 2.08: d1h7xd1, d1h7xd2, d1h7xd3, d1h7xd4, d1h7xd5
  • Heterogens: SF4, FMN, FAD, NDP, URF, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >1h7xA (A:)
    mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
    dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
    plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
    kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
    vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
    qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
    lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
    ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
    divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
    kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
    gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
    gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
    araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
    iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
    ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
    naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
    mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    vnpvc
    

    Sequence, based on observed residues (ATOM records): (download)
    >1h7xA (A:)
    apvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfdd
    ikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnp
    lgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqek
    mpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvv
    nfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdq
    gfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvfl
    vfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwne
    dedqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggd
    ivgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglk
    finpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmyg
    pgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasg
    adalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsia
    raakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtti
    aralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylk
    sieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqn
    aafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcym
    tcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrgl
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >1h7xB (B:)
    mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
    dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
    plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
    kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
    vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
    qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
    lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
    ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
    divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
    kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
    gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
    gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
    araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
    iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
    ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
    naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
    mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    vnpvc
    

    Sequence, based on observed residues (ATOM records): (download)
    >1h7xB (B:)
    apvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfdd
    ikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnp
    lgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqek
    mpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvv
    nfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdq
    gfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvfl
    vfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwne
    dedqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggd
    ivgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglk
    finpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmyg
    pgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasg
    adalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsia
    raakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtti
    aralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylk
    sieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqn
    aafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcym
    tcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >1h7xC (C:)
    mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
    dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
    plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
    kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
    vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
    qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
    lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
    ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
    divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
    kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
    gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
    gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
    araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
    iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
    ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
    naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
    mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    vnpvc
    

    Sequence, based on observed residues (ATOM records): (download)
    >1h7xC (C:)
    apvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfdd
    ikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnp
    lgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqek
    mpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvv
    nfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdq
    gfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvfl
    vfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwne
    dedqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggd
    ivgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglk
    finpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmyg
    pgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasg
    adalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsia
    raakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtti
    aralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylk
    sieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqn
    aafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcym
    tcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >1h7xD (D:)
    mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
    dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
    plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
    kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
    vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
    qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
    lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
    ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
    divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
    kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
    gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
    gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
    araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
    iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
    ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
    naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
    mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
    vnpvc
    

    Sequence, based on observed residues (ATOM records): (download)
    >1h7xD (D:)
    apvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfdd
    ikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnp
    lgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqek
    mpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvv
    nfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdq
    gfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvfl
    vfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwne
    dedqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggd
    ivgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglk
    finpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmyg
    pgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasg
    adalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsia
    raakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtti
    aralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylk
    sieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqn
    aafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcym
    tcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla