PDB entry 1e8d

View 1e8d on RCSB PDB site
Description: mechanistic aspects of cyanogenesis from active site mutant ser80ala of hydroxynitrile lyase from manihot esculenta in complex with acetone cyanohydrin
Class: lyase
Keywords: lyase, hydroxynitrile lyase, active site mutant, acetone cyanohydrin complex
Deposited on 2000-09-19, released 2001-08-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-07-24, with a file datestamp of 2019-07-19.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: hydroxynitrile lyase
    Species: MANIHOT ESCULENTA [TaxId:3983]
    Database cross-references and differences (RAF-indexed):
    • PDB 1E8D (0-4)
      • cloning artifact (0-4)
      • engineered mutation (83)
    • Uniprot P52705 (5-261)
    Domains in SCOPe 2.08: d1e8da1, d1e8da2
  • Chain 'B':
    Compound: hydroxynitrile lyase
    Species: MANIHOT ESCULENTA [TaxId:3983]
    Database cross-references and differences (RAF-indexed):
    • PDB 1E8D (Start-4)
      • cloning artifact (4)
      • engineered mutation (83)
    • Uniprot P52705 (5-261)
    Domains in SCOPe 2.08: d1e8db1, d1e8db2
  • Heterogens: CNH, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >1e8dA (A:)
piskmvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfde
yseplltfleklpqgekviivgeacaglniaiaadryvdkiaagvfhnsllpdtvhspsy
tvekllesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrk
gslfqnvlaqrpkftekgygsikkvyiwtdqdkiflpdfqrwqianykpdkvyqvqggdh
klqltkteevahilqevadaya

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1e8dB (B:)
piskmvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfde
yseplltfleklpqgekviivgeacaglniaiaadryvdkiaagvfhnsllpdtvhspsy
tvekllesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrk
gslfqnvlaqrpkftekgygsikkvyiwtdqdkiflpdfqrwqianykpdkvyqvqggdh
klqltkteevahilqevadaya

    Sequence, based on observed residues (ATOM records): (download) 
    >1e8dB (B:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgeacaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrkgslf
qnvlaqrpkftekgygsikkvyiwtdqdkiflpdfqrwqianykpdkvyqvqggdhklql
tkteevahilqevadaya