PDB entry 1e0u

View 1e0u on RCSB PDB site
Description: structure r271l mutant of e. coli pyruvate kinase
Class: phosphotransferase
Keywords: glycolysis, allostery
Deposited on 2000-04-10, released 2000-04-11
The last revision prior to the SCOP 1.73 freeze date was dated 2003-04-01, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.245
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • engineered mutation (270)
      • conflict (278)
    Domains in SCOP 1.73: d1e0ua1, d1e0ua2, d1e0ua3
  • Chain 'B':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • engineered mutation (270)
      • conflict (278)
    Domains in SCOP 1.73: d1e0ub1, d1e0ub2, d1e0ub3
  • Chain 'C':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • engineered mutation (270)
      • conflict (278)
    Domains in SCOP 1.73: d1e0uc1, d1e0uc2, d1e0uc3
  • Chain 'D':
    Compound: pyruvate kinase
    Species: ESCHERICHIA COLI
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14178 (0-469)
      • engineered mutation (270)
      • conflict (278)
    Domains in SCOP 1.73: d1e0ud1, d1e0ud2, d1e0ud3
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1e0uA (A:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0uA (A:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrleiteavcrgavetaekl
daplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddf
yrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1e0uB (B:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0uB (B:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrleiteavcrgavetaekl
daplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddf
yrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1e0uC (C:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0uC (C:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrleiteavcrgavetaekl
daplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddf
yrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1e0uD (D:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrlefnndnrklriteavcr
gavetaekldaplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlv
keitstddfyrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl

    Sequence, based on observed residues (ATOM records): (download) 
    >1e0uD (D:)
mkktkivctigpkteseemlakmldagmnvmrlnfshgdyaehgqriqnlrnvmsktgkt
aailldtkgpeirtmkleggndvslkagqtftfttdksvignsemvavtyegfttdlsvg
ntvlvddgligmevtaiegnkvickvlnngdlgenkgvnlpgvsialpalaekdkqdlif
gceqgvdfvaasfirkrsdvieirehlkahggenihiiskienqeglnnfdeileasdgi
mvargdlgveipveevifaqkmmiekciralkvvitatmmldsmiknprptraeagdvan
aildgtdavmlsgesakgkypleavsimaticertdrvmnsrleiteavcrgavetaekl
daplivvatqggksaravrkyfpdatilalttnektahqlvlskgvvpqlvkeitstddf
yrlgkelalqsglahkgdvvvmvsgalvpsgttntasvhvl