PDB entry 7o7w

View 7o7w on RCSB PDB site
Description: Crystal structure of rsEGFP2 mutant V151L in the non-fluorescent off-state the determined by serial femtosecond crystallography at room temperature
Class: fluorescent protein
Keywords: Reversibly photoswitchable fluorescent protein, FLUORESCENT PROTEIN
Deposited on 2021-04-13, released 2022-07-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2022-10-19, with a file datestamp of 2022-10-14.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (13-249)
      • initiating methionine (0)
      • expression tag (1-12)
      • insertion (14)
      • engineered mutation (77)
      • chromophore (78)
      • engineered mutation (80)
      • engineered mutation (161)
      • engineered mutation (174)
      • engineered mutation (217)
      • engineered mutation (242)
    Domains in SCOPe 2.08: d7o7wa1, d7o7wa2
  • Heterogens: PIA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7o7wA (A:)
mrgshhhhhhtdpmvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkf
icttgklpvpwptlvttlxvlcfsrypdhmkqhdffksampegyvqertiffkddgnykt
raevkfegdtlvnrielkgidfkedgnilghkleynynshnlyimadkqkngiksnfkir
hniedgsvqladhyqqntpigdgpvllpdnhylstqsklskdpnekrdhmvllefvtaag
itlgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >7o7wA (A:)
hhhtdpmvskgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgkl
pvpwptlvttlxvlcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfe
gdtlvnrielkgidfkedgnilghkleynynshnlyimadkqkngiksnfkirhniedgs
vqladhyqqntpigdgpvllpdnhylstqsklskdpnekrdhmvllefvtaagitlg