PDB entry 7kh4

View 7kh4 on RCSB PDB site
Description: Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H56W mutant with pyruvate bound in the active site and R,R-bislysine bound at the allosteric site
Class: lyase
Keywords: dihydrodipicolinate synthase, lyase
Deposited on 2020-10-19, released 2021-10-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-10-27, with a file datestamp of 2021-10-22.
Experiment type: XRAY
Resolution: 1.75 Å
R-factor: N/A
AEROSPACI score: 0.47 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4a_
  • Chain 'B':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4b1, d7kh4b2
  • Chain 'C':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4c_
  • Chain 'D':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4d1, d7kh4d2
  • Chain 'E':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4e_
  • Chain 'F':
    Compound: 4-hydroxy-tetrahydrodipicolinate synthase
    Species: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) [TaxId:192222]
    Gene: dapA, Cj0806
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9PPB4 (12-309)
      • expression tag (0-11)
      • engineered mutation (67)
    Domains in SCOPe 2.08: d7kh4f_
  • Heterogens: 3VN, ACT, EDO, GOL, KPI, MG, PEG, PG4, PGE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7kh4A (A:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4A (A:)
kniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatltweehrtc
ieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkptqqglyeh
ykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidkcvdllahe
prmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeakkindelyn
inkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkykikgf

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7kh4B (B:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4B (B:)
hhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatl
tweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkp
tqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidk
cvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeak
kindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkyk
ikgf

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7kh4C (C:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4C (C:)
kniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatltweehrtc
ieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkptqqglyeh
ykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidkcvdllahe
prmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeakkindelyn
inkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkykikgf

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7kh4D (D:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4D (D:)
hhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatl
tweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkp
tqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidk
cvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeak
kindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkyk
ikgf

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >7kh4E (E:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4E (E:)
dkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatltweehrt
cieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkptqqglye
hykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidkcvdllah
eprmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeakkindely
ninkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkykikgf

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >7kh4F (F:)
mrgshhhhhhgsmdkniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgtt
gesatltweehrtcieiavetckgtkvkvlagagsnatheavglakfakehgadgilsva
pyynkptqqglyehykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxea
sgnidkcvdllaheprmmlisgedainypilsnggkgvisvtsnllpdmisalthfalde
nykeakkindelyninkilfcesnpipiktamylaglieslefrlplcspskenfakiee
vmkkykikgf

    Sequence, based on observed residues (ATOM records): (download) 
    >7kh4F (F:)
kniiigamtalitpfkngkvdeqsyarlikrqiengidavvpvgttgesatltweehrtc
ieiavetckgtkvkvlagagsnatheavglakfakehgadgilsvapyynkptqqglyeh
ykaiaqsvdipvllynvpgrtgceistdtiiklfrdceniygvxeasgnidkcvdllahe
prmmlisgedainypilsnggkgvisvtsnllpdmisalthfaldenykeakkindelyn
inkilfcesnpipiktamylaglieslefrlplcspskenfakieevmkkykikgf