PDB entry 7kar

View 7kar on RCSB PDB site
Description: cryo-em structure of the sec complex from s. cerevisiae, sec63 fn3 mutant, class without sec62
Deposited on 2020-10-01, released 2021-01-06
The last revision was dated 2021-02-24, with a file datestamp of 2021-02-19.
Experiment type: EM
Resolution: 4 Å
R-factor: N/A
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Protein transport protein SEC61
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Protein transport protein SBH1
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Gene: SBH1, SEB1, YER087C-B, YER087BC
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: protein transport protein sss1
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: Protein translocation protein SEC63
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Gene: SEC63, NPL1, PTL1, YOR254C
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14906
      • engineered mutation (453)
      • engineered mutation (486)
  • Chain 'E':
    Compound: Translocation protein SEC66
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Translocation protein SEC72
    Species: Saccharomyces cerevisiae BY4741 [TaxId:1247190]
    Database cross-references and differences (RAF-indexed):

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7karA (A:)
mssnrvldlfkpfesflpeviaperkvpynqkliwtgvslliflilgqiplygivssets
dplywlramlasnrgtllelgvspiitssmifqflqgtqllqirpeskqdrelfqiaqkv
caiililgqalvvvmtgnygapsdlglpiclllifqlmfaslivmlldellskgyglgsg
islftatniaeqifwrafapttvnsgrgkefegaviaffhllavrkdkkralveafyrtn
lpnmfqvlmtvaiflfvlylqgfryelpirstkvrgqigiypiklfytsntpimlqsalt
sniflisqilfqkyptnplirligvwgirpgtqgpqmalsglayyiqplmslsealldpi
ktivyitfvlgscavfsktwieisgtsprdiakqfkdqgmvingkretsiyrelkkiipt
aaafggatigalsvgsdllgtlgsgasilmatttiygyyeaaakeggftknlvpgfsdlm

    Sequence, based on observed residues (ATOM records):
    >7karA (A:)
pfesflpeviaperkvpynqkliwtgvslliflilgqiplygivdplywlramlasnrgt
llelgvspiitssmifqflqgtqllqirpeskqdrelfqiaqkvcaiililgqalvvvmt
gnygaplpiclllifqlmfaslivmlldellskgyglgsgislftatniaeqifwrafap
ttvnsgrgkefegaviaffhllavrkdkkralveafyrtnlpnmfqvlmtvaiflfvlyl
qgfryelpirstkvrgqigiypiklfytsntpimlqsaltsniflisqilfqkyptnpli
rligvwgiqmalsglayyiqplmslsealldpiktivyitfvlgscavfsktwieisgts
prdiakqfkdqgmvingkretsiyrelkkiiptaaafggatigalsvgsdllgtlgsgas
ilmatttiygyyeaaakeggf

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7karB (B:)
mssptppggqrtlqkrkqgssqkvaasapkkntnsnnsilkiysdeatglrvdplvvlfl
avgfifsvvalhviskvagklf

    Sequence, based on observed residues (ATOM records):
    >7karB (B:)
rvdplvvlflavgfifsvvalhviskvagklf

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7karC (C:)
marasekgeekkqsnnqveklveapvefvregtqflakckkpdlkeytkivkavgigfia
vgiigyaiklihipiryviv

    Sequence, based on observed residues (ATOM records):
    >7karC (C:)
vefvregtqflakckkpdlkeytkivkavgigfiavgiigyaiklihipiryviv

  • Chain 'D':
    Sequence, based on SEQRES records:
    >7karD (D:)
ggsggsggsggsggsptnyeydeasetwpsfiltgllmvvgpmtllqiyqiffganaedg
nsgkskefneevfknlneeytsdeikqfrrkfdknsnkkskiwsrrniiiivgwilvail
lqrinsndaikdaatklfdpyeilgistsasdrdiksayrklsvkfhpdklakgltpdek
svmeetyvqitkayesltdelvrqnylkyghpdgpqstshgialprflvdgsaspllvvc
yvallglilpyfvsrwwartqsytkkgihnvtasnfvsnlvnykpseivttdlilhwlsf
ahefkqffpdlqptdfekllqdhinrrdsgklnnakfrivakchsllhglldiacgfrnl
dialgaintfkcivqavpltpncqilqlpnvdkehfitktgdihtlgklftledakigev
lgikdqaklnetlrvashipnlkiikadflvpgrpyislkvlvrsakqpliptslipeen
ltepqdsesqrdpfammskqplvpysfapffptkrrgswcclvssqkdgkilqtpiiiek
lsyknlnddkdffdkrikmdltkhekfdindweigtikiplgqpapetvgdfffrvivks
tdyfttdlditmnmkvrdspaveqvevyseeddeystdddetesddesdasdytdidtdt
eaeddespegenlyfq

    Sequence, based on observed residues (ATOM records):
    >7karD (D:)
tnyeydeasetwpsfiltgllmvvgpmtllqiyqfneevfknlneeytsdeikqfrrkfn
iiiivgwilvaillqrinsndaqstshgialprflvdgsaspllvvcyvallglilpyfv
srwwartqsytkkgihnvtasnfvsnlvnykpseivttdlilhwlsfahefkqffpdlqp
tdfekllqdhinrrdsgklnnakfrivakchsllhglldiacgfrnldialgaintfkci
vqavpltpncqilqlpnvdkehfitktgdihtlgklftledakigevlgikdqaklnetl
rvashipnlkiikadflvpgrpyislkvlvrsakqpliptslipeenltepqdsesqrdp
fammskqplvpysfapffptkrrgswcclvssqkdgkilqtpiiieklsyknlnddkdff
dkrikmdltkhekfdindweigtikiplgqpapetvgdfffrvivkstdyfttdlditmn
mkvrd

  • Chain 'E':
    Sequence, based on SEQRES records:
    >7karE (E:)
msefnetkfsnngtffeteepivetksisvytpliyvfilvvslvmfassyrkkqakkis
eqpsifdendahdlyfqikemsenekihekvlkaallnrgaesvrrslklkelapqinll
ykngsigedywkrfetevklielefkdtlqeaerlqpgwvqlfvmvckeicfnqalsrry
qsilkrkevcikewelkinndgrlvn

    Sequence, based on observed residues (ATOM records):
    >7karE (E:)
ndahdlyfqikemsenekihekvlkaallnrgaesvrrslklkelapqinllykngsige
dywkrfetevklielefkdtlqeaerlqpgwvqlfvmvckeicfnqalsrryqsilkrke
vcikewelkinndgrlvn

  • Chain 'F':
    Sequence, based on SEQRES records:
    >7karF (F:)
mvtleynansklitasdavvalstetnidqinvlttsligetnpnftpqpnealskmikg
lfesgmknlqqkklnealknvslaiemaqrkrapweafaiqlpelhfmlrskidlclilg
khlealqdldfllgtgliqpdvfvrkadcllklrqweearatcerglalapedmklrall
ietarnlaeynge

    Sequence, based on observed residues (ATOM records):
    >7karF (F:)
tleynansklitasdavvalstetnidqinvlttsligetnpnftpqpnealskmikglf
esgmknlqqkklnealknvslaiemaqrkrapweafaiqlpelhfmlrskidlclilgkh
lealqdldfllgtgliqpdvfvrkadcllklrqweearatcerglalapedmklrallie
tarnlaeyng