PDB entry 7f3v

View 7f3v on RCSB PDB site
Description: Crystal structure of YfiH with C107A mutation in complex with endogenous UDP-MurNAc
Class: hydrolase
Keywords: hydrolase, DUF152, amidase
Deposited on 2021-06-17, released 2021-12-29
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-12-29, with a file datestamp of 2021-12-24.
Experiment type: XRAY
Resolution: 1.47 Å
R-factor: N/A
AEROSPACI score: 0.57 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Purine nucleoside phosphorylase YfiH
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33644 (0-242)
      • engineered mutation (106)
      • expression tag (243-250)
    Domains in SCOPe 2.08: d7f3va_
  • Chain 'B':
    Compound: Purine nucleoside phosphorylase YfiH
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33644 (0-242)
      • engineered mutation (106)
      • expression tag (243-250)
    Domains in SCOPe 2.08: d7f3vb_
  • Chain 'C':
    Compound: Purine nucleoside phosphorylase YfiH
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33644 (0-242)
      • engineered mutation (106)
      • expression tag (243-250)
    Domains in SCOPe 2.08: d7f3vc_
  • Chain 'D':
    Compound: Purine nucleoside phosphorylase YfiH
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33644 (0-242)
      • engineered mutation (106)
      • expression tag (243-250)
    Domains in SCOPe 2.08: d7f3vd_
  • Heterogens: EPZ, PO4, TRS, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7f3vA (A:)
msklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnl
pskpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragtev
aaahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakas
aafiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfi
wlilehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7f3vA (A:)
sklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnlp
skpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragteva
aahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakasa
afiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfiw
li

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7f3vB (B:)
msklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnl
pskpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragtev
aaahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakas
aafiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfi
wlilehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7f3vB (B:)
klivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnlps
kpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragtevaa
ahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakasaa
fiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfiwl
i

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7f3vC (C:)
msklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnl
pskpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragtev
aaahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakas
aafiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfi
wlilehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7f3vC (C:)
sklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnlp
skpvwleqvhgkdvlkltgskradasysntpgtvcavmtadalpvlfcnragtevaaaha
gwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakasaafiq
hgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfiwli

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7f3vD (D:)
msklivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnl
pskpvwleqvhgkdvlkltgepyaskradasysntpgtvcavmtadalpvlfcnragtev
aaahagwrglcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakas
aafiqhgdkyladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfi
wlilehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7f3vD (D:)
klivpqwpqpkgvaacsstriggvslppydslnlgahcgdnpdhveenrkrlfaagnlps
kpvwleqvhgkdvlklkradasysntpgtvcavmtadalpvlfcnragtevaaahagwrg
lcagvleetvscfadnpenilawlgpaigprafevggevreafmavdakasaafiqhgdk
yladiyqlarqrlanvgveqifggdrctytenetffsyrrdkttgrmasfiwli