PDB entry 7ezk

View 7ezk on RCSB PDB site
Description: Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gs complex
Class: membrane protein
Keywords: Cholecystokinin A receptor, Gs complex, CCK-8, MEMBRANE PROTEIN
Deposited on 2021-06-01, released 2021-08-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-08-25, with a file datestamp of 2021-08-20.
Experiment type: EM
Resolution: 3.1 Å
R-factor: N/A
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Chimera of Guanine nucleotide-binding protein G(i) subunit alpha-1 and Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63096 (0-17)
    • Uniprot P63092 (18-58)
      • engineered mutation (41-42)
      • engineered mutation (55)
    • Uniprot P63096 (59-179)
    • Uniprot P63092 (180-360)
      • engineered mutation (202)
      • engineered mutation (225)
      • engineered mutation (228)
      • engineered mutation (238)
      • engineered mutation (332)
      • engineered mutation (338)
      • engineered mutation (341)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62873 (12-350)
      • expression tag (0-11)
    Domains in SCOPe 2.08: d7ezkb_
  • Chain 'D':
    Compound: Cholecystokinin receptor type A
    Species: Homo sapiens [TaxId:9606]
    Gene: CCKAR, CCKRA
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7ezkg_
  • Chain 'P':
    Compound: cholecystokinin-8
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 7EZK (0-8)
  • Heterogens: TYS

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7ezkB (B:)
mhhhhhhgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrr
tlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsg
nyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalw
dietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdi
naicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagy
ddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records): (download) 
    >7ezkB (B:)
seldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyam
hwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnic
siynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'D':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >7ezkG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records): (download) 
    >7ezkG (G:)
qarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfre

  • Chain 'P':
    No sequence available.