PDB entry 7e9h

View 7e9h on RCSB PDB site
Description: cryo-em structure of gi-bound metabotropic glutamate receptor mglu4
Deposited on 2021-03-04, released 2021-06-23
The last revision was dated 2021-07-07, with a file datestamp of 2021-07-02.
Experiment type: EM
Resolution: 4 Å
R-factor: N/A
AEROSPACI score: 0.1 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(i) subunit alpha-3
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAI3
    Database cross-references and differences (RAF-indexed):
    • Uniprot P08754 (0-353)
      • engineered mutation (46)
      • engineered mutation (190)
      • engineered mutation (202)
      • engineered mutation (244)
      • engineered mutation (325)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62873 (12-350)
      • expression tag (0-11)
  • Chain 'C':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: scFv16
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 7E9H (0-256)
  • Chain 'R':
    Compound: Metabotropic glutamate receptor 4
    Species: Homo sapiens [TaxId:9606]
    Gene: GRM4, GPRC1D, MGLUR4
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q14833 (10-889)
      • expression tag (0-9)
  • Chain 'S':
    Compound: Metabotropic glutamate receptor 4
    Species: Homo sapiens [TaxId:9606]
    Gene: GRM4, GPRC1D, MGLUR4
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q14833 (10-889)
      • expression tag (0-9)
  • Heterogens: SEP

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7e9hA (A:)
mgctlsaedkaaverskmidrnlredgekaakevkllllgagesgkntivkqmkiihedg
ysedeckqykvvvysntiqsiiaiiramgrlkidfgeaaraddarqlfvlagsaeegvmt
pelagvikrlwrdggvqacfsrsreyqlndsasyylndldrisqsnyiptqqdvlrtrvk
ttgivethftdkdlyfkmfdvgaqrserkkwihcfegvtaiifcvalsdydlvlaedeem
nrmhasmklfdsicnnkwftetsiilflnkkdlfeekikrsplticypeytgsntyeeaa
ayiqcqfedlnrrkdtkeiythftcstdtknvqfvfdavtdviiknnlkecgly

    Sequence, based on observed residues (ATOM records):
    >7e9hA (A:)
saedkaaverskmidrnlredgekaakevkllllgagesgkntivkqmkiigivethftd
kdlyfkmfdvgaqrserkkwihcfegvtaiifcvalsdydlvlaedeemnrmhasmklfd
sicnnkwftetsiilflnkkdlfeekikrsplticypeytgsntyeeaaayiqcqfedln
rrkdtkeiythftcstdtknvqfvfdavtdviiknnlkecgly

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7e9hB (B:)
mhhhhhhgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrr
tlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsg
nyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalw
dietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdi
naicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagy
ddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7e9hB (B:)
nnidpvgriqmrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaip
lrsswvmtcayapsgnyvacggldnicsiynlktregnvrvsrelaghtgylsccrfldd
nqivtssgdttcalwdietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvr
egmcrqtftghesdinaicffpngnafatgsddatcrlfdlradqelmtyshdniicgit
svsfsksgrlllagyddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswds
flkiwn

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7e9hC (C:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >7e9hC (C:)
kvskaaadlmayceahake

  • Chain 'D':
    Sequence, based on SEQRES records:
    >7e9hD (D:)
dvqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyy
adtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvs
sggggsggggsggggsdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqr
pgqspqlliyrmsnlasgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfg
agtklelkaaalevlfq

    Sequence, based on observed residues (ATOM records):
    >7e9hD (D:)
vqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyya
dtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvss
sdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqrpgqspqlliyrmsnl
asgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfgagtklel

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7e9hR (R:)
dykddddgapkpkghphmnsiridgditlgglfpvhgrgsegkpcgelkkekgihrleam
lfaldrinndpdllpnitlgarildtcsrdthaleqsltfvqaliekdgtevrcgsggpp
iitkpervvgvigasgssvsimvanilrlfkipqisyastapdlsdnsrydffsrvvpsd
tyqaqamvdivralkwnyvstvasegsygesgveafiqksredggvciaqsvkiprepka
gefdkiirrlletsnaraviifaneddirrvleaarranqtghffwmgsdswgskiapvl
hleevaegavtilpkrmsvrgfdryfssrtldnnrrniwfaefwednfhcklsrhalkkg
shvkkctnrerigqdsayeqegkvqfvidavyamghalhamhrdlcpgrvglcprmdpvd
gtqllkyirnvnfsgiagnpvtfnengdapgrydiyqyqlrndsaeykvigswtdhlhlr
iermhwpgsgqqlprsicslpcqpgerkktvkgmpccwhcepctgyqyqvdrytcktcpy
dmrptenrtgcrpipiiklewgspwavlplflavvgiaatlfvvitfvryndtpivkasg
relsyvllagiflcyattflmiaepdlgtcslrriflglgmsisyaalltktnriyrife
qgkrsvsaprfispasqlaitfslislqllgicvwfvvdpshsvvdfqdqrtldprfarg
vlkcdisdlslicllgysmllmvtctvyaiktrgvpetfneakpigftmyttcivwlafi
piffgtsqsadklyiqtttltvsvslsasvslgmlympkvyiilfhpeqnvpkrkrslka
vvtaatmsnkftqkgnfrpngeakselcenleapalatkqtyvtytnhai

    Sequence, based on observed residues (ATOM records):
    >7e9hR (R:)
nsiridgditlgglfpvhgrgsegkpcgelkkekgihrleamlfaldrinndpdllpnit
lgarildtcsrdthaleqsltfvqaliervvgvigasgssvsimvanilrlfkipqisya
stapdlsdnsrydffsrvvpsdtyqaqamvdivralkwnyvstvasegsygesgveafiq
ksredggvciaqsvkiprepkagefdkiirrlletsnaraviifaneddirrvleaarra
nqtghffwmgsdswgskiapvlhleevaegavtilpkrmsvrgfdryfssrtldnnrrni
wfaefwednfhcklvkkctnrerigqdsayeqegkvqfvidavyamghalhamhrdlcpg
rvglcprmdpvdgtqllkyirnvnfsgiagnpvtfnengdapgrydiyqyqlraeykvig
swtdhlhlriqqlprsicslpcqpgerkktvkgmpccwhcepctgyqyqvdrytcktcpy
dmrptenrtgcrpipiiklewgspwavlplflavvgiaatlfvvitfvryndtpivkasg
relsyvllagiflcyattflmiaepdlgtcslrriflglgmsisyaalltktnriyrife
qgkrsvsaprfispasqlaitfslislqllgicvwfvvdpshsvvdfqdqrtldprfarg
vlkcdisdlslicllgysmllmvtctvyaiktrgvpetfneakpigftmyttcivwlafi
piffgtsqsadklyiqtttltvsvslsasvslgmlympkvyiilfh

  • Chain 'S':
    Sequence, based on SEQRES records:
    >7e9hS (S:)
dykddddgapkpkghphmnsiridgditlgglfpvhgrgsegkpcgelkkekgihrleam
lfaldrinndpdllpnitlgarildtcsrdthaleqsltfvqaliekdgtevrcgsggpp
iitkpervvgvigasgssvsimvanilrlfkipqisyastapdlsdnsrydffsrvvpsd
tyqaqamvdivralkwnyvstvasegsygesgveafiqksredggvciaqsvkiprepka
gefdkiirrlletsnaraviifaneddirrvleaarranqtghffwmgsdswgskiapvl
hleevaegavtilpkrmsvrgfdryfssrtldnnrrniwfaefwednfhcklsrhalkkg
shvkkctnrerigqdsayeqegkvqfvidavyamghalhamhrdlcpgrvglcprmdpvd
gtqllkyirnvnfsgiagnpvtfnengdapgrydiyqyqlrndsaeykvigswtdhlhlr
iermhwpgsgqqlprsicslpcqpgerkktvkgmpccwhcepctgyqyqvdrytcktcpy
dmrptenrtgcrpipiiklewgspwavlplflavvgiaatlfvvitfvryndtpivkasg
relsyvllagiflcyattflmiaepdlgtcslrriflglgmsisyaalltktnriyrife
qgkrsvsaprfispasqlaitfslislqllgicvwfvvdpshsvvdfqdqrtldprfarg
vlkcdisdlslicllgysmllmvtctvyaiktrgvpetfneakpigftmyttcivwlafi
piffgtsqsadklyiqtttltvsvslsasvslgmlympkvyiilfhpeqnvpkrkrslka
vvtaatmsnkftqkgnfrpngeakselcenleapalatkqtyvtytnhai

    Sequence, based on observed residues (ATOM records):
    >7e9hS (S:)
nsiridgditlgglfpvhgrgsegkpcgelkkekgihrleamlfaldrinndpdllpnit
lgarildtcsrdthaleqsltfvqalervvgvigasgssvsimvanilrlfkipqisyas
tapdlsdnsrydffsrvvpsdtyqaqamvdivralkwnyvstvasegsygesgveafiqk
sredggvciaqsvkiprepkagefdkiirrlletsnaraviifaneddirrvleaarran
qtghffwmgsdswgskiapvlhleevaegavtilpkrmsvrgfdryfssrtldnnrrniw
faefwednfhckkkctnrerigqdsayeqegkvqfvidavyamghalhamhrdlcpgrvg
lcprmdpvdgtqllkyirnvnfsgiagnpvtfnengdapgrydiyqyqlrndsaeykvig
swtdhlhlriermhwlprsicslpcqpgerkktvkgmpccwhcepctgyqyqvdrytckt
cpydmrptenrtgcrpipiiklewgspwavlplflavvgiaatlfvvitfvryndtpivk
asgrelsyvllagiflcyattflmiaepdlgtcslrriflglgmsisyaalltktnriyr
ifeqrfispasqlaitfslislqllgicvwfvvdpshsvvdfqdqrtldprfargvlkcd
isdlslicllgysmllmvtctvyaiktrgvpetfneakpigftmyttcivwlafipiffg
tsqsadklyiqtttltvsvslsasvslgmlympkvyiilfh