PDB entry 7e8q

View 7e8q on RCSB PDB site
Description: crystal structure of a flavin-dependent monooxygenase hada f441v mutant complexed with reduced fad and 4-nitrophenol
Deposited on 2021-03-02, released 2021-07-21
The last revision was dated 2021-08-11, with a file datestamp of 2021-08-06.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Chlorophenol monooxygenase
    Species: Ralstonia pickettii [TaxId:329]
    Gene: hadA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q53008 (0-516)
      • engineered mutation (440)
  • Chain 'B':
    Compound: Chlorophenol monooxygenase
    Species: Ralstonia pickettii [TaxId:329]
    Gene: hadA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q53008 (0-516)
      • engineered mutation (440)
  • Chain 'C':
    Compound: Chlorophenol monooxygenase
    Species: Ralstonia pickettii [TaxId:329]
    Gene: hadA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q53008 (0-516)
      • engineered mutation (440)
  • Chain 'D':
    Compound: Chlorophenol monooxygenase
    Species: Ralstonia pickettii [TaxId:329]
    Gene: hadA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q53008 (0-516)
      • engineered mutation (440)
  • Heterogens: FDA, NPO, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7e8qA (A:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi
dsavtddteyrktadyakaldaarhqeevalagamai

    Sequence, based on observed residues (ATOM records):
    >7e8qA (A:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7e8qB (B:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi
dsavtddteyrktadyakaldaarhqeevalagamai

    Sequence, based on observed residues (ATOM records):
    >7e8qB (B:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7e8qC (C:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi
dsavtddteyrktadyakaldaarhqeevalagamai

    Sequence, based on observed residues (ATOM records):
    >7e8qC (C:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi

  • Chain 'D':
    Sequence, based on SEQRES records:
    >7e8qD (D:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi
dsavtddteyrktadyakaldaarhqeevalagamai

    Sequence, based on observed residues (ATOM records):
    >7e8qD (D:)
mirtgtqyleslndgrnvwvgnekidnvathpktrdyaqrhadfydlhhrpdlqdvmtyi
deggqrramqwfghrdkeqlrrkrkyhetvmremagasfprtpdvnnyvlttyiddpapw
etqsigddghikagkivdfiryarehdlncapqfvdpqmdrsnpdaqerspglrvvekne
kgivvngvkaigtgvafadwihigvffrpgipgdqvifaatpvntpgvtivcreslvkdd
kvehplaaqgdeldgmtvfenvfipwshvfhignpnhaklypqrvfdwlhyhalirqmvr
aelvaglavlitehigtnkipavqtrvakligfhqamlahliaseelgfhtpgghykpni
liydfgralylenfsqmiyelvdlsgrsalifasedqwnddklngwfermnngpvgrphd
rvkigrvirdlfltdwgsrlvvfenfngtplqgirmltmqraefsgsgpygklarqvcgi
ds