PDB entry 7e8i

View 7e8i on RCSB PDB site
Description: Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin
Class: nuclear protein
Keywords: BRCA1, nucleosome, DNA damage, chromatin, BARD1, NUCLEAR PROTEIN
Deposited on 2021-03-01, released 2021-06-30
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-07-14, with a file datestamp of 2021-07-09.
Experiment type: EM
Resolution: 3.1 Å
R-factor: N/A
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: histone h3
    Species: Xenopus laevis [TaxId:8355]
    Gene: XELAEV_18002543mg
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7e8ia_
  • Chain 'B':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7e8ib_
  • Chain 'C':
    Compound: histone h2a
    Species: Xenopus laevis [TaxId:8355]
    Gene: h2ac14.L, h2ac14, hist1h2aj, LOC494591, XELAEV_18003602mg
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6AZJ8 (0-128)
      • engineered mutation (14)
    Domains in SCOPe 2.08: d7e8ic_
  • Chain 'D':
    Compound: Histone H2B 1.1
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P02281 (0-121)
      • engineered mutation (28)
    Domains in SCOPe 2.08: d7e8id_
  • Chain 'E':
    Compound: histone h3
    Species: Xenopus laevis [TaxId:8355]
    Gene: XELAEV_18002543mg
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7e8ie_
  • Chain 'F':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7e8if_
  • Chain 'G':
    Compound: histone h2a
    Species: Xenopus laevis [TaxId:8355]
    Gene: h2ac14.L, h2ac14, hist1h2aj, LOC494591, XELAEV_18003602mg
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6AZJ8 (0-128)
      • engineered mutation (14)
    Domains in SCOPe 2.08: d7e8ig_
  • Chain 'H':
    Compound: Histone H2B 1.1
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P02281 (0-121)
      • engineered mutation (28)
    Domains in SCOPe 2.08: d7e8ih_
  • Chain 'I':
    Compound: DNA (145-mer)
    Species: Homo sapiens [TaxId:9606]
  • Chain 'J':
    Compound: DNA (145-mer)
    Species: Homo sapiens [TaxId:9606]
  • Chain 'K':
    Compound: brca1-associated ring domain protein 1
    Species: Homo sapiens [TaxId:9606]
    Gene: BARD1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99728 (3-355)
      • expression tag (0-2)
  • Chain 'L':
    Compound: Polyubiquitin-B
    Species: Homo sapiens [TaxId:9606]
    Gene: UBB
    Database cross-references and differences (RAF-indexed):
    • Uniprot J3QS39 (10-85)
      • expression tag (0-9)
      • engineered mutation (85)
    Domains in SCOPe 2.08: d7e8il_

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7e8iA (A:)
artkqtarkstggkaprkqlatkaarksapatggvkkphryrpgtvalreirryqkstel
lirklpfqrlvreiaqdfktdlrfqssavmalqeaseaylvalfedtnlcaihakrvtim
pkdiqlarrirgera

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iA (A:)
phryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavmalqease
aylvalfedtnlcaihakrvtimpkdiqlarrirge

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7e8iB (B:)
sgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkv
flenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iB (B:)
ggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavty
tehakrktvtamdvvyalkrqgrtlygfg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7e8iC (C:)
sgrgkqggktrakactrssraglqfpvgrvhrllrkgnyaervgagapvylaavleylta
eilelagnaardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpkkt
essksaksk

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iC (C:)
trakactrssraglqfpvgrvhrllrkgnyaervgagapvylaavleyltaeilelagna
ardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllp

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7e8iD (D:)
aksapapkkgskkavtktqkkdgkkrrktrkesyaiyvykvlkqvhpdtgisskamsimn
sfvndvferiageasrlahynkrstitsreiqtavrlllpgelakhavsegtkavtkyts
ak

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iD (D:)
rkesyaiyvykvlkqvhpdtgisskamsimnsfvndvferiageasrlahynkrstitsr
eiqtavrlllpgelakhavsegtkavtkytsa

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >7e8iE (E:)
artkqtarkstggkaprkqlatkaarksapatggvkkphryrpgtvalreirryqkstel
lirklpfqrlvreiaqdfktdlrfqssavmalqeaseaylvalfedtnlcaihakrvtim
pkdiqlarrirgera

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iE (E:)
kphryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavmalqeas
eaylvalfedtnlcaihakrvtimpkdiqlarrirgera

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >7e8iF (F:)
sgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkv
flenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iF (F:)
rkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavtytehakr
ktvtamdvvyalkrqgrtlygfgg

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >7e8iG (G:)
sgrgkqggktrakactrssraglqfpvgrvhrllrkgnyaervgagapvylaavleylta
eilelagnaardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpkkt
essksaksk

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iG (G:)
kactrssraglqfpvgrvhrllrkgnyaervgagapvylaavleyltaeilelagnaard
nkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllp

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >7e8iH (H:)
aksapapkkgskkavtktqkkdgkkrrktrkesyaiyvykvlkqvhpdtgisskamsimn
sfvndvferiageasrlahynkrstitsreiqtavrlllpgelakhavsegtkavtkyts
ak

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iH (H:)
trkesyaiyvykvlkqvhpdtgisskamsimnsfvndvferiageasrlahynkrstits
reiqtavrlllpgelakhavsegtkavtkytsa

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >7e8iL (L:)
ghmdydipttmqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkql
edgrtlsdyniqkestlhlvlrlrgc

    Sequence, based on observed residues (ATOM records): (download) 
    >7e8iL (L:)
mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
iqkestlhlvlrlrgc