PDB entry 7dhr

View 7dhr on RCSB PDB site
Description: cryo-em structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-gs protein complex.
Deposited on 2020-11-17, released 2020-12-16
The last revision was dated 2020-12-16, with a file datestamp of 2020-12-11.
Experiment type: EM
Resolution: 3.8 Å
R-factor: N/A
AEROSPACI score: 0.08 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Bos taurus [TaxId:9913]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nb35 nanobody
    Species: Lama glama [TaxId:9844]
    Database cross-references and differences (RAF-indexed):
    • PDB 7DHR
  • Chain 'R':
    Compound: Beta-2 adrenergic receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: ADRB2, ADRB2R, B2AR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07550
      • engineered mutation (100)
      • engineered mutation (119)
      • engineered mutation (121)
      • engineered mutation (145)
      • engineered mutation (148)
      • engineered mutation (210)
      • engineered mutation (293)
      • engineered mutation (335)
  • Heterogens: 5FW

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7dhrA (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqm
rilhvngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvela
npenqfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfld
kidvikqadyvpsdqdllrcrvltsgifetkfqvdkvnfhmfdvggqrderrkwiqcfnd
vtaiifvvasssynmvirednqtnrlqealnlfksiwnnrwlrtisvilflnkqdllaek
vlagkskiedyfpefaryttpedatpepgedprvtrakyfirdeflristasgdgrhycy
phftcavdtenirrvfndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7dhrA (A:)
dqrneekaqreankkiekqlqkdkqvyrathrllllgfetkfqvdkvnfhmfdvggqrde
rrkwiqcfndvtaiifvvasnrlqealnlfksiwnnrwlrtisvilflnkqdllaekvle
dyfpefaryttpedaedprvtrakyfirdeflristasgdgrhycyphftnirrvfndcr
diiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7dhrB (B:)
mseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiya
mhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldni
csiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttf
tghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngna
fatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdal
kadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7dhrB (B:)
rqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyamhwgtds
rllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnicsiynlv
rvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttftghtgdvmslsla
pdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnafatgsddatcrlf
dlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalkadragvlaghdn
rvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7dhrG (G:)
mhhhhhhmasnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltp
vpasenpfrekkffsail

    Sequence, based on observed residues (ATOM records):
    >7dhrG (G:)
arklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp

  • Chain 'N':
    Sequence, based on SEQRES records:
    >7dhrN (N:)
mkyllptaaagllllaaqpamamqvqlqesggglvqpggslrlscaasgftfsnykmnwv
rqapgkglewvsdisqsgasisytgsvkgrftisrdnakntlylqmnslkpedtavyyca
rcpapftrdcfdvtsttyayrgqgtqvtvsshhhhhh

    Sequence, based on observed residues (ATOM records):
    >7dhrN (N:)
qvqlqesggqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisytgs
vkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgqgtq
vtv

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7dhrR (R:)
mktiialsyifslvfadykddddamgqpgngsafllapnrshapdhdvtqqrdevwvvgm
givmslivlaivfgnvlvitaiakferlqtvtnyfitslavadlvmglavvpfgaahilt
ktwtfgnfwcefwtsidvlcvtasiwtlvviavdryfaitspfkyqslltknkarviilm
vwivsgltsflpiqmhwyrathqeaincyaeetccdfftnqayaiassivsfyvplvimv
fvysrvfqeakrqlqkidksegrfhvqnrsskfalkehkalktlgiimgtftlawlpffi
vnivhviqdnlirkevyillnwigyvnsgfnpliysrspdfriafqellclrrsslkhhh
hhhhhhh

    Sequence, based on observed residues (ATOM records):
    >7dhrR (R:)
evwvvgmgivmslivlaivfgnvlvitaiakferlqtvtnyfitslavadlvmglavvpf
gaahiltktwtfgnfwcefwtsidvlcvtasiwtlvviavdryfaitspfkyqslltknk
arviilmvwivsgltsflpiqmhwyrahqeaincyaeetccdfftnqayaiassivsfyv
plvimvfvysrvfqeakrqlqkidksegrflkehkalktlgiimgtftlawlpffivniv
hviqdnlirkevyillnwigyvnsgfnpliysrspdfriafqel