PDB entry 7d3s

View 7d3s on RCSB PDB site
Description: human secr in complex with an engineered gs heterotrimer
Deposited on 2020-09-20, released 2020-11-04
The last revision was dated 2020-12-30, with a file datestamp of 2020-12-25.
Experiment type: EM
Resolution: 2.9 Å
R-factor: N/A
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092 (Start-379)
      • engineered mutation (44-45)
      • engineered mutation (244)
      • engineered mutation (247)
      • engineered mutation (357)
      • engineered mutation (360)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Rattus norvegicus [TaxId:10116]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P54311 (12-350)
      • expression tag (11)
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: nanobody Nb35
    Species: unidentified [TaxId:32644]
    Database cross-references and differences (RAF-indexed):
    • PDB 7D3S
  • Chain 'P':
    Compound: Secretin
    Species: HOMO SAPIENS, synthetic [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: Secretin receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: SCTR
    Database cross-references and differences (RAF-indexed):

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7d3sA (A:)
gnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgadnsgkstivkqmriyh
vngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvelanpen
qfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfldkidv
ikqadyvpsdqdllrcrvltsgifetkfqvdkvnfhmfdvggqrderrkwiqcfndvtai
ifvvdssdynrlqealnlfksiwnnrwlrtisvilflnkqdllaekvlagkskiedyfpe
faryttpedatpepgedprvtrakyfirdeflristasgdgrhycyphftcavdtenarr
ifndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7d3sA (A:)
qrneekaqreankkiekqlqkdkqvyrathrllllgadnsgkstivkqmrigifetkfqv
dkvnfhmfdvggqrderrkwiqcfndvtaiifvvdssdynrlqealnlfksiwnnrwlrt
isvilflnkqdllaekvlagkskiedyfpefaryttpedatpepgedprvtrakyfirde
flristasgdgrhycyphftcavdtenarrifndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7d3sB (B:)
mhhhhhhgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrr
tlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsg
nyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalw
dietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdi
naicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagy
ddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7d3sB (B:)
qseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiya
mhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldni
csiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttf
tghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngna
fatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdal
kadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7d3sG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffs

    Sequence, based on observed residues (ATOM records):
    >7d3sG (G:)
tasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence, based on SEQRES records:
    >7d3sN (N:)
mgqvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasi
sytgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayr
gqgtqvtvsslhhhhhh

    Sequence, based on observed residues (ATOM records):
    >7d3sN (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtvss

  • Chain 'P':
    Sequence; same for both SEQRES and ATOM records:
    >7d3sP (P:)
hsdgtftselsrlregarlqrllqglv

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7d3sR (R:)
dykddddamghstgalprlcdvlqvlweeqdqclqelsreqtgdlgteqpvpgcegmwdn
iscwpssvpgrmvevecprflrmltsrngslfrnctqdgwsetfprpnlacgvnvndssn
ekrhsyllklkvmytvgyssslvmllvalgilcafrrlhctrnyihmhlfvsfilralsn
fikdavlfssddvtycdahragcklvmvlfqycimanyswllveglylhtllaisffser
kylqgfvafgwgspaifvalwaiarhfledvgcwdinanasiwwiirgpvilsilinfil
finilrilmrklrtqetrgnevshykrlarstllliplfgihyivfafspedameiqlff
elalgsfqglvvavlycflngevqlevqkkwqqwhlrefplhpvasfsnstkashleqsq
gtcrtsiienlyfg

    Sequence, based on observed residues (ATOM records):
    >7d3sR (R:)
ssnekrhsyllklkvmytvgyssslvmllvalgilcafrrlhctrnyihmhlfvsfilra
lsnfikdavlfssdahragcklvmvlfqycimanyswllveglylhtllaisffserkyl
qgfvafgwgspaifvalwaiarhfledvgcwdinanasiwwiirgpvilsilinfilfin
ilrilmrklrtqevshykrlarstllliplfgihyivfafspedameiqlffelalgsfq
glvvavlycflngevqlevqkkwqqw