PDB entry 7cz5

View 7cz5 on RCSB PDB site
Description: cryo-em structure of the human growth hormone-releasing hormone receptor-gs protein complex
Deposited on 2020-09-07, released 2020-11-18
The last revision was dated 2020-11-18, with a file datestamp of 2020-11-13.
Experiment type: EM
Resolution: 2.6 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092 (Start-393)
      • engineered mutation (53)
      • engineered mutation (225)
      • engineered mutation (267)
      • engineered mutation (270)
      • engineered mutation (273)
      • engineered mutation (279)
      • engineered mutation (283-284)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Rattus norvegicus [TaxId:10116]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nanobody35
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 7CZ5 (0-125)
  • Chain 'P':
    Compound: Somatoliberin
    Species: HOMO SAPIENS, synthetic [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: GHRHR
    Database cross-references and differences (RAF-indexed):
  • Heterogens: PLM, CLR, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7cz5A (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqm
rilhvngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvela
npenqfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfld
kidvikqadyvpsdqdllrcrvltsgifetkfqvdkvnfhmfdvgaqrderrkwiqcfnd
vtaiifvvasssynmvirednqtnrlqaalklfdsiwnnkwlrdtsvilflnkqdllaek
vlagkskiedyfpefaryttpedatpepgedprvtrakyfirdeflristasgdgrhycy
phftcavdtenirrvfndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7cz5A (A:)
tedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqmsgifetkf
qvdkvnfhmfdvgaqrderrkwiqcfndvtaiifvvasstnrlqaalklfdsiwnnkwlr
dtsvilflnkqdllaekvlakiedyfpefaryttpedatpepgedprvtrakyfirdefl
ristasgdgrhycyphftcavdtenirrvfndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7cz5B (B:)
mhhhhhhssglvprgshmashhhhhhhhhhgsllqseldqlrqeaeqlknqirdarkaca
datlsqitnnidpvgriqmrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyt
tnkvhaiplrsswvmtcayapsgnyvacggldnicsiynlktregnvrvsrelaghtgyl
sccrflddnqivtssgdttcalwdietgqqtttftghtgdvmslslapdtrlfvsgacda
saklwdvregmcrqtftghesdinaicffpngnafatgsddatcrlfdlradqelmtysh
dniicgitsvsfsksgrlllagyddfncnvwdalkadragvlaghdnrvsclgvtddgma
vatgswdsflkiwngssggggsggggssgvsgwrlfkkis

    Sequence, based on observed residues (ATOM records):
    >7cz5B (B:)
eldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyamh
wgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnics
iynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttftg
htgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnafa
tgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalka
dragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7cz5G (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >7cz5G (G:)
tasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence; same for both SEQRES and ATOM records:
    >7cz5N (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtv

  • Chain 'P':
    Sequence, based on SEQRES records:
    >7cz5P (P:)
yadaiftnsyrkvlgqlsarkllqdimsrqqgesnqergararl

    Sequence, based on observed residues (ATOM records):
    >7cz5P (P:)
yadaiftnsyrkvlgqlsarkllqdims

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7cz5R (R:)
hmhpecdfitqlredesaclqaaeempnttlgcpatwdgllcwptagsgewvtlpcpdff
shfssesgavkrdctitgwsepfppypvacpvplellaeeesyfstvkiiytvghsisiv
alfvaitilvalrrlhcprnyvhtqlfttfilkagavflkdaalfhsddtdhcsfstvlc
kvsvaashfatmtnfswllaeavylncllastspssrrafwwlvlagwglpvlftgtwvs
cklafediacwdlddtspywwiikgpivlsvgvnfglflniirilvrklepaqgslhtqs
qywrlskstlfliplfgihyiifnflpdnaglgirlplelglgsfqgfivailycflnqe
vrteisrkwhghdpellpawrtrgssggggsggggssgvftledfvgdweqtaaynldqv
leqggvssllqnlavsvtpiqrivrsgenalkidihviipyeglsadqmaqieevfkvvy
pvddhhfkvilpygtlvidgvtpnmlnyfgrpyegiavfdgkkitvtgtlwngnkiider
litpdgsmlfrvtins

    Sequence, based on observed residues (ATOM records):
    >7cz5R (R:)
laeeesyfstvkiiytvghsisivalfvaitilvalrrlhcprnyvhtqlfttfilkaga
vflkdaalfhsddtdhcsfstvlckvsvaashfatmtnfswllaeavylncllastspss
rrafwwlvlagwglpvlftgtwvscklafediacwdlddtspywwiikgpivlsvgvnfg
lflniirilvrkletqsqywrlskstlfliplfgihyiifnflpdnaglgirlplelglg
sfqgfivailycflnqevrteisrkwhgh