PDB entry 7cx2

View 7cx2 on RCSB PDB site
Description: cryo-em structure of the pge2-bound ep2-gs complex
Deposited on 2020-09-01, released 2021-05-05
The last revision was dated 2021-05-05, with a file datestamp of 2021-04-30.
Experiment type: EM
Resolution: 2.8 Å
R-factor: N/A
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092
      • engineered mutation (53)
      • engineered mutation (225)
      • engineered mutation (267)
      • engineered mutation (270)
      • engineered mutation (273)
      • engineered mutation (279)
      • engineered mutation (283-284)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62873 (19-357)
      • expression tag (18)
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nanobody-35
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 7CX2 (0-127)
  • Chain 'R':
    Compound: Prostaglandin E2 receptor EP2 subtype
    Species: Homo sapiens [TaxId:9606]
    Gene: PTGER2
    Database cross-references and differences (RAF-indexed):
  • Heterogens: P2E, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7cx2A (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqm
rilhvngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvela
npenqfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfld
kidvikqadyvpsdqdllrcrvltsgifetkfqvdkvnfhmfdvgaqrderrkwiqcfnd
vtaiifvvasssynmvirednqtnrlqaalklfdsiwnnkwlrdtsvilflnkqdllaek
vlagkskiedyfpefaryttpedatpepgedprvtrakyfirdeflristasgdgrhycy
phftcavdtenirrvfndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7cx2A (A:)
qrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqmsgifetkfqvd
kvnfhmfdvgaqrderrkwiqcfndvtaiifvvasssynrlqaalklfdsiwnnkwlrdt
svilflnkqdllaekvlagkskiedyfpefaryttpedatpepgedprvtrakyfirdef
lristasgdgrhycyphftcavdtenirrvfndcrdiiqrmhlrqyel

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7cx2B (B:)
mhhhhhhlevlfqgpgssqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgr
iqmrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmt
cayapsgnyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssg
dttcalwdietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtf
tghesdinaicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksg
rlllagyddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7cx2B (B:)
qseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiya
mhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldni
csiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttf
tghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngna
fatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdal
kadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7cx2G (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >7cx2G (G:)
ntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence; same for both SEQRES and ATOM records:
    >7cx2N (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtvss

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7cx2R (R:)
mgnasndsqsedcetrqwlppgespaissvmfsagvlgnlialallarrwrgdvgcsagr
rsslslfhvlvtelvftdllgtclispvvlasyarnqtlvalapesractyfafamtffs
latmlmlfamalerylsighpyfyqrrvsrsgglavlpviyavsllfcslplldygqyvq
ycpgtwcfirhgrtaylqlyatlllllivsvlacnfsvilnlirmhrrsrrsrcgpslgs
grggpgarrrgervsmaeetdhlillaimtitfavcslpftifaymnetssrkekwdlqa
lrflsinsiidpwvfailrppvlrlmrsvlccrislrtqdatqtscstqsdaskqadl

    Sequence, based on observed residues (ATOM records):
    >7cx2R (R:)
espaissvmfsagvlgnlialallarslfhvlvtelvftdllgtclispvvlasyarnqt
lvalapesractyfafamtffslatmlmlfamalerylsighpyfyqrrvsrsgglavlp
viyavsllfcslplldygqyvqycpgtwcfirhgrtaylqlyatlllllivsvlacnfsv
ilnlirmhrrsraeetdhlillaimtitfavcslpftifaymnetssrkekwdlqalrfl
sinsiidpwvfailrppvlrlmrsvl