PDB entry 7ctr

View 7ctr on RCSB PDB site
Description: Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H
Class: hydrolase
Keywords: PROTEIN ENGINEERING, POLYESTERASE, disulfide bond, metal binding, HYDROLASE
Deposited on 2020-08-20, released 2021-02-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-04-14, with a file datestamp of 2021-04-09.
Experiment type: XRAY
Resolution: 1.2 Å
R-factor: N/A
AEROSPACI score: 0.73 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Alpha/beta hydrolase family protein
    Species: Saccharomonospora viridis [TaxId:1852]
    Gene: Cut190, SAMN02982918_2340
    Database cross-references and differences (RAF-indexed):
    • Uniprot W0TJ64 (2-259)
      • expression tag (1)
      • engineered mutation (78)
      • engineered mutation (93)
      • engineered mutation (157)
      • engineered mutation (181)
      • engineered mutation (183)
      • engineered mutation (205)
      • engineered mutation (251)
    Domains in SCOPe 2.08: d7ctra1, d7ctra2
  • Chain 'B':
    Compound: Alpha/beta hydrolase family protein
    Species: Saccharomonospora viridis [TaxId:1852]
    Gene: Cut190, SAMN02982918_2340
    Database cross-references and differences (RAF-indexed):
    • Uniprot W0TJ64 (2-259)
      • expression tag (1)
      • engineered mutation (78)
      • engineered mutation (93)
      • engineered mutation (157)
      • engineered mutation (181)
      • engineered mutation (183)
      • engineered mutation (205)
      • engineered mutation (251)
    Domains in SCOPe 2.08: d7ctrb1, d7ctrb2
  • Heterogens: DIO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7ctrA (A:)
gpnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavavap
gftasqgsmswygervashgfivftidtntrldapgqrgrqllaaldylversdrkvrer
ldpnrlavmghsmggggsleatvmrpslkasipltpwhldktwgqvqvptfiigaeldti
apvsthakpfyeslpsslpkaymelcgathfapnipnttiakyviswlkrfvdedtrysq
flcpnptdraiceyrstcpykln

    Sequence, based on observed residues (ATOM records): (download) 
    >7ctrA (A:)
pnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavavapg
ftasqgsmswygervashgfivftidtntrldapgqrgrqllaaldylversdrkvrerl
dpnrlavmghsmggggsleatvmrpslkasipltpwhldktwgqvqvptfiigaeldtia
pvsthakpfyeslpsslpkaymelcgathfapnipnttiakyviswlkrfvdedtrysqf
lcpnptdraiceyrstcpy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7ctrB (B:)
gpnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavavap
gftasqgsmswygervashgfivftidtntrldapgqrgrqllaaldylversdrkvrer
ldpnrlavmghsmggggsleatvmrpslkasipltpwhldktwgqvqvptfiigaeldti
apvsthakpfyeslpsslpkaymelcgathfapnipnttiakyviswlkrfvdedtrysq
flcpnptdraiceyrstcpykln

    Sequence, based on observed residues (ATOM records): (download) 
    >7ctrB (B:)
pnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavavapg
ftasqgsmswygervashgfivftidtntrldapgqrgrqllaaldylversdrkvrerl
dpnrlavmghsmggggsleatvmrpslkasipltpwhldktwgqvqvptfiigaeldtia
pvsthakpfyeslpsslpkaymelcgathfapnipnttiakyviswlkrfvdedtrysqf
lcpnptdraiceyrstcpy