PDB entry 7crp

View 7crp on RCSB PDB site
Description: nsd3 bearing e1181k/t1232a dual mutation in complex with 187-bp ncp (1:1 binding mode)
Deposited on 2020-08-14, released 2020-10-21
The last revision was dated 2021-03-03, with a file datestamp of 2021-02-26.
Experiment type: EM
Resolution: 3.2 Å
R-factor: N/A
AEROSPACI score: 0.17 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA (168-mer)
    Species: XENOPUS LAEVIS [TaxId:8355]
  • Chain 'B':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: histone h2a
    Species: Xenopus laevis [TaxId:8355]
    Gene: hist1h2aj, LOC494591, XELAEV_18003602mg
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: histone h2b
    Species: Xenopus tropicalis [TaxId:8364]
    Gene: h2bc12, hist1h2bk
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: histone h3
    Species: Xenopus laevis [TaxId:8355]
    Gene: h3c8.S, h3c8, H3l, hist1h3g
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q92133
      • engineered mutation (89)
      • engineered mutation (119)
  • Chain 'F':
    Compound: histone h4
    Species: Xenopus laevis [TaxId:8355]
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: histone h2a
    Species: Xenopus laevis [TaxId:8355]
    Gene: hist1h2aj, LOC494591, XELAEV_18003602mg
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: histone h2b
    Species: Xenopus tropicalis [TaxId:8364]
    Gene: h2bc12, hist1h2bk
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: Histone-lysine N-methyltransferase NSD3
    Species: Homo sapiens [TaxId:9606]
    Gene: NSD3, WHSC1L1, DC28
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9BZ95
      • engineered mutation (501)
      • engineered mutation (552)
  • Chain 'K':
    Compound: DNA (168-mer)
    Species: XENOPUS LAEVIS [TaxId:8355]
  • Chain 'M':
    Compound: histone h3
    Species: Xenopus laevis [TaxId:8355]
    Gene: h3c8.S, h3c8, H3l, hist1h3g
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q92133
      • engineered mutation (35)
      • engineered mutation (89)
      • engineered mutation (119)
  • Heterogens: NLE, ZN, SAM

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7crpB (B:)
sgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkv
flenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records):
    >7crpB (B:)
kvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavtytehakrk
tvtamdvvyalkrqgrtlygfg

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7crpC (C:)
sgrgkqggktrakaktrssraglqfpvgrvhrllrkgnyaervgagapvylaavleylta
eilelagnaardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpkkt
essksaksk

    Sequence, based on observed residues (ATOM records):
    >7crpC (C:)
trakaktrssraglqfpvgrvhrllrkgnyaervgagapvylaavleyltaeilelagna
ardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpk

  • Chain 'D':
    Sequence, based on SEQRES records:
    >7crpD (D:)
aksapapkkgskkavtktqkkdgkkrrktrkesyaiyvykvlkqvhpdtgisskamsimn
sfvndvferiageasrlahynkrstitsreiqtavrlllpgelakhavsegtkavtkyts
ak

    Sequence, based on observed residues (ATOM records):
    >7crpD (D:)
rktrkesyaiyvykvlkqvhpdtgisskamsimnsfvndvferiageasrlahynkrsti
tsreiqtavrlllpgelakhavsegtkavtkytsa

  • Chain 'E':
    Sequence, based on SEQRES records:
    >7crpE (E:)
artkqtarkstggkaprkqlatkaarksapatggvlkphryrpgtvalreirryqkstel
lirklpfqrlvreiaqdfktdlrfqssavlalqeaseaylvglfedtnlcgihakrvtil
pkdiqlarrirgera

    Sequence, based on observed residues (ATOM records):
    >7crpE (E:)
kphryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavlalqeas
eaylvglfedtnlcgihakrvtilpkdiqlarrirger

  • Chain 'F':
    Sequence, based on SEQRES records:
    >7crpF (F:)
sgrgkggkglgkggakrhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkv
flenvirdavtytehakrktvtamdvvyalkrqgrtlygfgg

    Sequence, based on observed residues (ATOM records):
    >7crpF (F:)
rhrkvlrdniqgitkpairrlarrggvkrisgliyeetrgvlkvflenvirdavtyteha
krktvtamdvvyalkrqgrtlygfgg

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7crpG (G:)
sgrgkqggktrakaktrssraglqfpvgrvhrllrkgnyaervgagapvylaavleylta
eilelagnaardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpkkt
essksaksk

    Sequence, based on observed residues (ATOM records):
    >7crpG (G:)
trakaktrssraglqfpvgrvhrllrkgnyaervgagapvylaavleyltaeilelagna
ardnkktriiprhlqlavrndeelnkllgrvtiaqggvlpniqsvllpkk

  • Chain 'H':
    Sequence, based on SEQRES records:
    >7crpH (H:)
aksapapkkgskkavtktqkkdgkkrrktrkesyaiyvykvlkqvhpdtgisskamsimn
sfvndvferiageasrlahynkrstitsreiqtavrlllpgelakhavsegtkavtkyts
ak

    Sequence, based on observed residues (ATOM records):
    >7crpH (H:)
krrktrkesyaiyvykvlkqvhpdtgisskamsimnsfvndvferiageasrlahynkrs
titsreiqtavrlllpgelakhavsegtkavtkytsa

  • Chain 'I':
    Sequence, based on SEQRES records:
    >7crpI (I:)
vsdvqsmdsslsrrgtgmskkdtvcqicessgdslipcegecckhfhleclglaslpdsk
ficmecktgqhpcfsckvsgkdvkrcsvgacgkfyheacvrkfptaifeskgfrcpqhcc
sacsmekdihkaskgrmmrclrcpvayhsgdaciaagsmlvssyilicsnhskrssnssa
vnvgfcfvcarglivqdhsdpmfssyaykshyllnesnraelmklpmipsssaskkkcek
ggrllccescpasfhpeclsiempegcwncndckagkklhykqivwvklgnyrwwpaeic
nprsvplniqglkhdlgdfpvfffgshdyywvhqgrvfpyvegdksfaegqtsinktfkk
aleeaakrfqelkaqreskealeieknsrkpppykhikankvigkvqiqvadlseiprcn
ckpadenpcgleseclnrmlqyechpqvcpagdrcqnqcftkrlypdaeiikterrgwgl
rtkrsikkgefvneyvgelidkeecrlrikrahensvtnfymltvtkdriidagpkgnys
rfmnhscnpnceaqkwtvngdvrvglfalcdipagmeltfnynldclgngrtechcgadn
csgflgvrpksacastneekaknaklkqkrrkiktepkqmhedycfqcgdggelvmcdkk
dcpkayhllclnltqppygkwecpwhqcdecssaavsfcefcphsfckdhekgalvpsal
egrlccsehdpmapvspeywskikckwesqdhgeevke

    Sequence, based on observed residues (ATOM records):
    >7crpI (I:)
nsrkpppykhikankvigkvqiqvadlseiprcnckpadenpcgleseclnrmlqyechp
qvcpagdrcqnqcftkrlypdaeiikterrgwglrtkrsikkgefvneyvgelidkeecr
lrikrahensvtnfymltvtkdriidagpkgnysrfmnhscnpnceaqkwtvngdvrvgl
falcdipagmeltfnynldclgngrtechcgadncsgflgvr

  • Chain 'K':
    No sequence available.

  • Chain 'M':
    Sequence, based on SEQRES records:
    >7crpM (M:)
artkqtarkstggkaprkqlatkaarksapatggvlkphryrpgtvalreirryqkstel
lirklpfqrlvreiaqdfktdlrfqssavlalqeaseaylvglfedtnlcgihakrvtil
pkdiqlarrirgera

    Sequence, based on observed residues (ATOM records):
    >7crpM (M:)
tggvlkphryrpgtvalreirryqkstellirklpfqrlvreiaqdfktdlrfqssavla
lqeaseaylvglfedtnlcgihakrvtilpkdiqlarrirger