PDB entry 7crh

View 7crh on RCSB PDB site
Description: cryo-em structure of skf83959 bound dopamine receptor drd1-gs signaling complex
Deposited on 2020-08-13, released 2021-03-03
The last revision was dated 2021-03-03, with a file datestamp of 2021-02-26.
Experiment type: EM
Resolution: 3.3 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092 (Start-393)
      • engineered mutation (204)
      • engineered mutation (225)
      • engineered mutation (365)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62873 (17-355)
      • expression tag (16)
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nanobody35
    Species: Lama glama [TaxId:9844]
    Database cross-references and differences (RAF-indexed):
    • PDB 7CRH
  • Chain 'R':
    Compound: D(1A) dopamine receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: DRD1
    Database cross-references and differences (RAF-indexed):
  • Heterogens: GBU

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7crhA (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqm
rilhvngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvela
npenqfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfld
kidvikqadyvpsdqdllrcrvlttgifetkfqvdkvnfhmfdvgaqrderrkwiqcfnd
vtaiifvvasssynmvirednqtnrlqealnlfksiwnnrwlrtisvilflnkqdllaek
vlagkskiedyfpefaryttpedatpepgedprvtrakyfirdeflristasgdgrhycy
phftcsvdtenirrvfndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7crhA (A:)
dqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqmriltgifetk
fqvdkvnfhmfdvgaqrderrkwiqcfndvtaiifvvasssynmtnrlqealnlfksiwn
nrwlrtisvilflnkqdllaekvlagkskiedyfpefaryttpedatpepgedprvtrak
yfirdeflristasgdgrhycyphftcsvdtenirrvfndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7crhB (B:)
mhhhhlevlfqgpgssgseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriq
mrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtca
yapsgnyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdt
tcalwdietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftg
hesdinaicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrl
llagyddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7crhB (B:)
gseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiya
mhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldni
csiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttf
tghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngna
fatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdal
kadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >7crhG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >7crhG (G:)
ntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence, based on SEQRES records:
    >7crhN (N:)
mkyllptaaagllllaaqpamaqvqlqesggglvqpggslrlscaasgftfsnykmnwvr
qapgkglewvsdisqsgasisytgsvkgrftisrdnakntlylqmnslkpedtavyycar
cpapftrdcfdvtsttyayrgqgtqvtvsshhhhhh

    Sequence, based on observed residues (ATOM records):
    >7crhN (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtvss

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7crhR (R:)
mrtlntsamdgtglvverdfsvriltacflsllilstllgntlvcaavirfrhlrskvtn
ffvislavsdllvavlvmpwkavaeiagfwpfgsfcniwvafdimcstasilnlcvisvd
rywaisspfryerkmtpkaafilisvawtlsvlisfipvqlswhkakptspsdgnatsla
etidncdsslsrtyaisssvisfyipvaimivtytriyriaqkqirriaaleraavhakn
cqtttgngkpvecsqpessfkmsfkretkvlktlsvimgvfvccwlpffilncilpfcgs
getqpfcidsntfdvfvwfgwansslnpiiyafnadfrkafstllgcyrlcpatnnaiet
vsinnngaamfsshheprgsiskecnlvyliphavgssedlkkeeaagiarpleklspal
svildydtdvslekiqpitqngqhpt

    Sequence, based on observed residues (ATOM records):
    >7crhR (R:)
fsvriltacflsllilstllgntlvcaavirfrhlrskvtnffvislavsdllvavlvmp
wkavaeiagfwpfgsfcniwvafdimcstasilnlcvisvdrywaisspfryerkmtpka
afilisvawtlsvlisfipvqlswhkncdsslsrtyaisssvisfyipvaimivtytriy
riaqkqirriaaleraavhsfkretkvlktlsvimgvfvccwlpffilncilpfccidsn
tfdvfvwfgwansslnpiiyafnadfrkafstl