PDB entry 7clz

View 7clz on RCSB PDB site
Description: Crystal structure of Alp1U W187F/Y247F in complex with fluostatin C
Class: hydrolase
Keywords: Substrate, Complex, Biosynthesis, HYDROLASE
Deposited on 2020-07-22, released 2020-10-07
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-12-30, with a file datestamp of 2020-12-25.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Putative hydrolase
    Species: Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516) [TaxId:278992]
    Gene: SAMT0137, SAMT0138
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1RQU8 (Start-338)
      • engineered mutation (206)
      • engineered mutation (266)
    Domains in SCOPe 2.08: d7clza_
  • Chain 'B':
    Compound: Putative hydrolase
    Species: Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516) [TaxId:278992]
    Gene: SAMT0137, SAMT0138
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1RQU8 (Start-338)
      • engineered mutation (206)
      • engineered mutation (266)
    Domains in SCOPe 2.08: d7clzb_
  • Chain 'C':
    Compound: Putative hydrolase
    Species: Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516) [TaxId:278992]
    Gene: SAMT0137, SAMT0138
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1RQU8 (Start-338)
      • engineered mutation (206)
      • engineered mutation (266)
    Domains in SCOPe 2.08: d7clzc_
  • Chain 'D':
    Compound: Putative hydrolase
    Species: Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516) [TaxId:278992]
    Gene: SAMT0137, SAMT0138
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1RQU8 (Start-338)
      • engineered mutation (206)
      • engineered mutation (266)
    Domains in SCOPe 2.08: d7clzd_
  • Heterogens: DY9, NA, MLT, BO3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7clzA (A:)
mgsshhhhhhssglvprgshmlalgtgatalavtgspaaahpgphpgpvpsdrelarslp
ggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpqlarryhviavdlrgm
ggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafafaanhpeatrkvall
dtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrhvidwlyansladqs
lvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmpvlgiggnftfedlr
nkltaqatdvhmvrasksvhylpeeepdvvagalldffg

    Sequence, based on observed residues (ATOM records): (download) 
    >7clzA (A:)
gpvpsdrelarslpggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpql
arryhviavdlrgmggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafaf
aanhpeatrkvalldtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrh
vidwlyansladqslvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmp
vlgiggnftfedlrnkltaqatdvhmvrasksvhylpeeepdvvagalldffg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7clzB (B:)
mgsshhhhhhssglvprgshmlalgtgatalavtgspaaahpgphpgpvpsdrelarslp
ggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpqlarryhviavdlrgm
ggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafafaanhpeatrkvall
dtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrhvidwlyansladqs
lvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmpvlgiggnftfedlr
nkltaqatdvhmvrasksvhylpeeepdvvagalldffg

    Sequence, based on observed residues (ATOM records): (download) 
    >7clzB (B:)
gpvpsdrelarslpggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpql
arryhviavdlrgmggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafaf
aanhpeatrkvalldtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrh
vidwlyansladqslvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmp
vlgiggnftfedlrnkltaqatdvhmvrasksvhylpeeepdvvagalldffg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7clzC (C:)
mgsshhhhhhssglvprgshmlalgtgatalavtgspaaahpgphpgpvpsdrelarslp
ggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpqlarryhviavdlrgm
ggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafafaanhpeatrkvall
dtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrhvidwlyansladqs
lvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmpvlgiggnftfedlr
nkltaqatdvhmvrasksvhylpeeepdvvagalldffg

    Sequence, based on observed residues (ATOM records): (download) 
    >7clzC (C:)
gpvpsdrelarslpggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpql
arryhviavdlrgmggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafaf
aanhpeatrkvalldtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrh
vidwlyansladqslvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmp
vlgiggnftfedlrnkltaqatdvhmvrasksvhylpeeepdvvagalldffg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7clzD (D:)
mgsshhhhhhssglvprgshmlalgtgatalavtgspaaahpgphpgpvpsdrelarslp
ggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpqlarryhviavdlrgm
ggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafafaanhpeatrkvall
dtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrhvidwlyansladqs
lvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmpvlgiggnftfedlr
nkltaqatdvhmvrasksvhylpeeepdvvagalldffg

    Sequence, based on observed residues (ATOM records): (download) 
    >7clzD (D:)
gpvpsdrelarslpggfrsrharvggvrlhyvsgghgeplllvpgwpqtwwayrkvmpql
arryhviavdlrgmggsdkpaggydkktmaadlhalvrglghrqvnvaghdigsmvafaf
aanhpeatrkvalldtphpdqseyemrilcrpgtgttlwwfafnqlqalpeqlmhgrmrh
vidwlyansladqslvgdldrdiyanaynspqavragtrwfqachqditdqagygkltmp
vlgiggnftfedlrnkltaqatdvhmvrasksvhylpeeepdvvagalldffg