PDB entry 7ci0

View 7ci0 on RCSB PDB site
Description: Microbial Hormone-sensitive lipase E53 mutant S162A
Class: hydrolase
Keywords: Esterase, Microbial Hormone-sensitive lipase, hydrolase
Deposited on 2020-07-06, released 2021-07-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-07-14, with a file datestamp of 2021-07-09.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: N/A
AEROSPACI score: 0.49 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-313)
      • engineered mutation (161)
    Domains in SCOPe 2.08: d7ci0a_
  • Chain 'B':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-313)
      • engineered mutation (161)
    Domains in SCOPe 2.08: d7ci0b_
  • Chain 'C':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-313)
      • engineered mutation (161)
    Domains in SCOPe 2.08: d7ci0c_
  • Chain 'D':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-313)
      • engineered mutation (161)
    Domains in SCOPe 2.08: d7ci0d_
  • Heterogens: NPO, 6NA, PEG, EDO, DMS, DIO, GOL, SO4, PGE, NA, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7ci0A (A:)
mtdtpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlsc
pgpagdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdy
rlapehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpa
dvpvvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdh
taapptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdl
eriiaamkmmlgta

    Sequence, based on observed residues (ATOM records): (download) 
    >7ci0A (A:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7ci0B (B:)
mtdtpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlsc
pgpagdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdy
rlapehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpa
dvpvvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdh
taapptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdl
eriiaamkmmlgta

    Sequence, based on observed residues (ATOM records): (download) 
    >7ci0B (B:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7ci0C (C:)
mtdtpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlsc
pgpagdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdy
rlapehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpa
dvpvvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdh
taapptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdl
eriiaamkmmlgta

    Sequence, based on observed residues (ATOM records): (download) 
    >7ci0C (C:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7ci0D (D:)
mtdtpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlsc
pgpagdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdy
rlapehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpa
dvpvvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdh
taapptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdl
eriiaamkmmlgta

    Sequence, based on observed residues (ATOM records): (download) 
    >7ci0D (D:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg