PDB entry 7cfn

View 7cfn on RCSB PDB site
Description: cryo-em structure of the int-777-bound gpbar-gs complex
Deposited on 2020-06-27, released 2020-09-09
The last revision was dated 2021-04-07, with a file datestamp of 2021-04-02.
Experiment type: EM
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092 (Start-393)
      • engineered mutation (53)
      • engineered mutation (225)
      • engineered mutation (267)
      • engineered mutation (270)
      • engineered mutation (273)
      • engineered mutation (279)
      • engineered mutation (283-284)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nanobody-35
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 7CFN (0-127)
  • Chain 'R':
    Compound: G-protein coupled bile acid receptor 1
    Species: Homo sapiens [TaxId:9606]
    Gene: GPBAR1, TGR5
    Database cross-references and differences (RAF-indexed):
  • Heterogens: FX0, CLR, PLM, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7cfnA (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqm
rilhvngfngeggeedpqaarsnsdgekatkvqdiknnlkeaietivaamsnlvppvela
npenqfrvdyilsvmnvpdfdfppefyehakalwedegvracyersneyqlidcaqyfld
kidvikqadyvpsdqdllrcrvltsgifetkfqvdkvnfhmfdvgaqrderrkwiqcfnd
vtaiifvvasssynmvirednqtnrlqaalklfdsiwnnkwlrdtsvilflnkqdllaek
vlagkskiedyfpefaryttpedatpepgedprvtrakyfirdeflristasgdgrhycy
phftcavdtenirrvfndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >7cfnA (A:)
tedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkntivkqmsgifetkf
qvdkvnfhmfdvgaqrderrkwiqcfndvtaiifvvasssynrlqaalklfdsiwnnkwl
rdtsvilflnkqdllaekvlagkskiedyfpefaryttpedatpepgedprvtrakyfir
deflristasgdgrhycyphftcavdtenirrvfndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7cfnB (B:)
mhhhhhhlevlfqgpgssgseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgr
iqmrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmt
cayapsgnyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssg
dttcalwdietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtf
tghesdinaicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksg
rlllagyddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >7cfnB (B:)
seldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyam
hwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnic
siynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records:
    >7cfnG (G:)
ntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence; same for both SEQRES and ATOM records:
    >7cfnN (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtvss

  • Chain 'R':
    Sequence, based on SEQRES records:
    >7cfnR (R:)
mtpnstgevpspipkgalglslalasliitanlllalgiawdrrlrsppagcfflsllla
glltglalptlpglwnqsrrgywscllvylapnfsflsllanlllvhgerymavlrplqp
pgsirlallltwagpllfaslpalgwnhwtpgancssqaifpapylylevyglllpavga
aaflsvrvlatahrqlqdicrleravcrdepsalaraltwrqaraqagamllfglcwgpy
vatlllsvlayeqrpplgpgtllsllslgsasaaavpvamglgdqrytapwraaaqrclq
glwgrasrdspgpsiayhpssqssvdldln

    Sequence, based on observed residues (ATOM records):
    >7cfnR (R:)
lglslalasliitanlllalgiawdrrlrsppagcfflslllaglltglalptlpglwnq
srrgywscllvylapnfsflsllanlllvhgerymavlrplqppgsirlallltwagpll
faslpalgwnhwtpgancssqaifpapylylevyglllpavgaaaflsvrvlatahrqlq
dicrleravcrdepsalaraltwrqaraqagamllfglcwgpyvatlllsvlayeqrppl
gpgtllsllslgsasaaavpvamglgdqrytapw