PDB entry 7cef
View 7cef on RCSB PDB site
Description: Crystal structure of PET-degrading cutinase Cut190 /S226P/R228S/ mutant with the C-terminal three residues deletion
Class: hydrolase
Keywords: PROTEIN ENGINEERING, THERMOSTABILITY, POLYESTERASE, Multiple conformation, HYDROLASE
Deposited on
2020-06-23, released
2020-08-26
The last revision prior to the SCOPe 2.08 freeze date was dated
2021-04-07, with a file datestamp of
2021-04-02.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.52
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: Alpha/beta hydrolase family protein
Species: Saccharomonospora viridis [TaxId:1852]
Gene: Cut190, SAMN02982918_2340
Database cross-references and differences (RAF-indexed):
- Uniprot W0TJ64 (2-261)
- expression tag (0-1)
- engineered mutation (183)
- engineered mutation (185)
Domains in SCOPe 2.08: d7cefa1, d7cefa2 - Chain 'B':
Compound: Alpha/beta hydrolase family protein
Species: Saccharomonospora viridis [TaxId:1852]
Gene: Cut190, SAMN02982918_2340
Database cross-references and differences (RAF-indexed):
- Uniprot W0TJ64 (2-261)
- expression tag (0-1)
- engineered mutation (183)
- engineered mutation (185)
Domains in SCOPe 2.08: d7cefb1, d7cefb2 - Heterogens: CA, ZN, HOH
PDB Chain Sequences:
- Chain 'A':
Sequence; same for both SEQRES and ATOM records: (download)
>7cefA (A:)
gpqdnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavav
apgftasqgsmswygervasqgfivftidtntrldqpgqrgrqllaaldylversdrkvr
erldpnrlavmghsmggggsleatvmrpslkasipltpwnldktwgqvqvptfiigaeld
tiapvsthakpfyeslpsslpkaymeldgathfapnipnttiakyviswlkrfvdedtry
sqflcpnptdraieeyrstcpy
- Chain 'B':
Sequence; same for both SEQRES and ATOM records: (download)
>7cefB (B:)
gpqdnpyergpdptedsieairgpfsvatervssfasgfgggtiyypretdegtfgavav
apgftasqgsmswygervasqgfivftidtntrldqpgqrgrqllaaldylversdrkvr
erldpnrlavmghsmggggsleatvmrpslkasipltpwnldktwgqvqvptfiigaeld
tiapvsthakpfyeslpsslpkaymeldgathfapnipnttiakyviswlkrfvdedtry
sqflcpnptdraieeyrstcpy