PDB entry 7c97

View 7c97 on RCSB PDB site
Description: cryo-em structure of an escherichia coli rnap-promoter open complex (rpo) with sspa
Deposited on 2020-06-05, released 2020-08-12
The last revision was dated 2020-08-26, with a file datestamp of 2020-08-21.
Experiment type: EM
Resolution: 3.68 Å
R-factor: N/A
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Escherichia coli [TaxId:562]
    Gene: rpoA, ECPG_02615
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Escherichia coli [TaxId:562]
    Gene: rpoA, ECPG_02615
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: DNA-directed RNA polymerase subunit beta
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A8V2 (Start-1341)
      • engineered mutation (515)
  • Chain 'D':
    Compound: DNA-directed RNA polymerase subunit beta'
    Species: Escherichia coli [TaxId:562]
    Gene: rpoC
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: DNA-directed RNA polymerase subunit omega
    Species: Escherichia coli [TaxId:562]
    Gene: rpoZ
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: RNA polymerase sigma factor rpoD
    Species: Escherichia coli [TaxId:562]
    Gene: rpoD
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: DNA (63-mer)
    Species: Escherichia coli, synthetic [TaxId:562]
  • Chain 'H':
    Compound: DNA (63-mer)
    Species: Escherichia coli, synthetic [TaxId:562]
  • Chain 'I':
    Compound: stringent starvation protein A
    Species: Escherichia coli [TaxId:562]
    Gene: sspA
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: stringent starvation protein A
    Species: Escherichia coli [TaxId:562]
    Gene: sspA
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Escherichia coli [TaxId:562]
    Gene: rpoA, ECPG_02615
    Database cross-references and differences (RAF-indexed):
  • Heterogens: MG, ZN

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7c97A (A:)
mqgsvteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteve
idgvlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgd
veivkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspv
eriaynveaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrdvrqp
evkeekpefdpillrpvddleltvrsanclkaeaihyigdlvqrtevellktpnlgkksl
teikdvlasrglslgmrlenwppasiade

    Sequence, based on observed residues (ATOM records):
    >7c97A (A:)
eflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteveidgvlh
eystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgdveivkp
qhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspveriayn
veaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrd

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7c97B (B:)
mqgsvteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteve
idgvlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgd
veivkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspv
eriaynveaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrdvrqp
evkeekpefdpillrpvddleltvrsanclkaeaihyigdlvqrtevellktpnlgkksl
teikdvlasrglslgmrlenwppasiade

    Sequence, based on observed residues (ATOM records):
    >7c97B (B:)
teflkprlvdieqvthakvtleplergfghtlgnalrrillssmpgcavteveidgvlhe
ystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgdveivkpq
hvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspveriaynv
eaarveqrtdldklviemetngtidpeeairraatilaeqleafvd

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7c97C (C:)
mvysytekkrirkdfgkrpqvldvpyllsiqldsfqkfieqdpegqygleaafrsvfpiq
sysgnselqyvsyrlgepvfdvqecqirgvtysaplrvklrlviyereapegtvkdikeq
evymgeiplmtdngtfvingtervivsqlhrspgvffdsdkgkthssgkvlynariipyr
gswldfefdpkdnlfvridrrrklpatiilralnytteqildlffekvifeirdnklqme
lvperlrgetasfdieangkvyvekgrritarhirqlekddvklievpveyiagkvvakd
yidestgelicaanmelsldllaklsqsghkrietlftndldhgpyisetlrvdptndrl
salveiyrmmrpgepptreaaeslfenlffsedrydlsavgrmkfnrsllreeiegsgil
skddiidvmkklidirngkgevddidhlgnrrirsvgemaenqfrvglvrveravkerls
lgdldtlmpqdminakpisaavkeffgssqlsqfmvqnnplseithkrrisalgpggltr
eragfevrdvhpthygrvcpietpegpniglinslsvyaqtneygfletpyrkvtdgvvt
deihylsaieegnyviaqansnldeeghfvedlvtcrskgesslfsrdqvdymdvstqqv
vsvgaslipflehddanralmganmqrqavptlradkplvgtgmeravavdsgvtavakr
ggvvqyvdasrivikvnedemypgeagidiynltkytrsnqntcinqmpcvslgepverg
dvladgpstdlgelalgqnmrvafmpwngynfedsilvservvqedrfttihiqelacvs
rdtklgpeeitadipnvgeaalskldesgivyigaevtggdilvgkvtpkgetqltpeek
llraifgekasdvkdsslrvpngvsgtvidvqvftrdgvekdkraleieemqlkqakkdl
seelqileaglfsriravlvaggveaekldklprdrwlelgltdeekqnqleqlaeqyde
lkhefekkleakrrkitqgddlapgvlkivkvylavkrriqpgdkmagrhgnkgviskin
piedmpydengtpvdivlnplgvpsrmnigqilethlgmaakgigdkinamlkqqqevak
lrefiqraydlgadvrqkvdlstfsdeevmrlaenlrkgmpiatpvfdgakeaeikellk
lgdlptsgqirlydgrtgeqferpvtvgymymlklnhlvddkmharstgsyslvtqqplg
gkaqfggqrfgemevwaleaygaaytlqemltvksddvngrtkmyknivdgnhqmepgmp
esfnvllkeirslginielede

    Sequence, based on observed residues (ATOM records):
    >7c97C (C:)
ysytekkrirkdfgkrpqvldvpyllsiqldsfqkfieqdpegqygleaafrsvfpiqsy
sgnselqyvsyrlgepvfdvqecqirgvtysaplrvklrlviyereapegtvkdikeqev
ymgeiplmtdngtfvingtervivsqlhrspgvffdsdkgkthssgkvlynariipyrgs
wldfefdpkdnlfvridrrrklpatiilralnytteqildlffekvifeirdnklqmelv
perlrgetasfdieangkvyvekgrritarhirqlekddvklievpveyiagkvvakdyi
destgelicaanmelsldllaklsqsghkrietlftndldhgpyisetlrvdptndrlsa
lveiyrmmrpgepptreaaeslfenlffsedrydlsavgrmkfnrsllreeiegsgilsk
ddiidvmkklidirngkgevddidhlgnrrirsvgemaenqfrvglvrveravkerlslg
dldtlmpqdminakpisaavkeffgssqlsqfmvqnnplseithkrrisalgpggltrer
agfevrdvhpthygrvcpietpegpniglinslsvyaqtneygfletpyrkvtdgvvtde
ihylsaieegnyviaqansnldeeghfvedlvtcrskgesslfsrdqvdymdvstqqvvs
vgaslipflehddanralmganmqrqavptlradkplvgtgmeravavdsgvtavakrgg
vvqyvdasrivikvnedemypgeagidiynltkytrsnqntcinqmpcvslgepvergdv
ladgpstdlgelalgqnmrvafmpwngynfedsilvservvqedrfttihiqelacvsrd
tklgpeeitadipnvgeaalskldesgivyigaevtggdilvgkvtpkgetqltpeekll
raifgekasdvkdsslrvpngvsgtvidvqvftrdgvekdkraleieemqlkqakkdlse
elqileaglfsriravlvaggveaekldklprdrwlelgltdeekqnqleqlaeqydelk
hefekkleakrrkitqgddlapgvlkivkvylavkrriqpgdkmagrhgnkgviskinpi
edmpydengtpvdivlnplgvpsrmnigqilethlgmaakgigdkinamlkqqqevaklr
efiqraydlgadvrqkvdlstfsdeevmrlaenlrkgmpiatpvfdgakeaeikellklg
dlptsgqirlydgrtgeqferpvtvgymymlklnhlvddkmharstgsyslvtqqplggk
aqfggqrfgemevwaleaygaaytlqemltvksddvngrtkmyknivdgnhqmepgmpes
fnvllkeirslginielede

  • Chain 'D':
    Sequence, based on SEQRES records:
    >7c97D (D:)
mkdllkflkaqtkteefdaikialaspdmirswsfgevkkpetinyrtfkperdglfcar
ifgpvkdyeclcgkykrlkhrgvicekcgvevtqtkvrrermghielasptahiwflksl
psrigllldmplrdiervlyfesyvvieggmtnlerqqilteeqyldaleefgdefdakm
gaeaiqallksmdleqeceqlreelnetnsetkrkkltkriklleafvqsgnkpewmilt
vlpvlppdlrplvpldggrfatsdlndlyrrvinrnnrlkrlldlaapdiivrnekrmlq
eavdalldngrrgraitgsnkrplksladmikgkqgrfrqnllgkrvdysgrsvitvgpy
lrlhqcglpkkmalelfkpfiygklelrglattikaakkmvereeavvwdildevirehp
vllnraptlhrlgiqafepvliegkaiqlhplvcaaynadfdgdqmavhvpltleaqlea
ralmmstnnilspangepiivpsqdvvlglyymtrdcvnakgegmvltgpkeaerlyrsg
laslharvkvriteyekdangelvaktslkdttvgrailwmivpkglpysivnqalgkka
iskmlntcyrilglkptvifadqimytgfayaarsgasvgiddmvipekkheiiseaeae
vaeiqeqfqsglvtagerynkvidiwaaandrvskammdnlqtetvinrdgqeekqvsfn
siymmadsgargsaaqirqlagmrglmakpdgsiietpitanfreglnvlqyfisthgar
kgladtalktansgyltrrlvdvaqdlvvteddcgthegimmtpvieggdvkeplrdrvl
grvtaedvlkpgtadilvprntllheqwcdlleensvdavkvrsvvscdtdfgvcahcyg
rdlarghiinkgeaigviaaqsigepgtqltmrtfhiggaasraaaessiqvknkgsikl
snvksvvnssgklvitsrntelklidefgrtkesykvpygavlakgdgeqvaggetvanw
dphtmpvitevsgfvrftdmidgqtitrqtdeltglsslvvldsaertaggkdlrpalki
vdaqgndvlipgtdmpaqyflpgkaivqledgvqissgdtlaripqesggtkditgglpr
vadlfearrpkepailaeisgivsfgketkgkrrlvitpvdgsdpyeemipkwrqlnvfe
gervergdvisdgpeaphdilrlrgvhavtryivnevqdvyrlqgvkindkhievivrqm
lrkativnagssdflegeqveysrvkianreleangkvgatysrdllgitkaslatesfi
saasfqettrvlteaavagkrdelrglkenvivgrlipagtgyayhqdrmrrraageapa
apqvtaedasaslaellnaglggsdne

    Sequence, based on observed residues (ATOM records):
    >7c97D (D:)
teefdaikialaspdmirswsfgevkkpetinyrtfkperdglfcarifgpvkdyeclcg
kykrlkhrgvicekcgvevtqtkvrrermghielasptahiwflkslpsrigllldmplr
diervlyfesyvvieggmtnlerqqilteeqyldaleefgdefdakmgaeaiqallksmd
leqeceqlreelnetnsetkrkkltkriklleafvqsgnkpewmiltvlpvlppdlrplv
pldggrfatsdlndlyrrvinrnnrlkrlldlaapdiivrnekrmlqeavdalldngrrg
raitgsnkrplksladmikgkqgrfrqnkrvdysgrsvitvgpylrlhqcglpkkmalel
fkpfiygklelrglattikaakkmvereeavvwdildevirehpvllnraptlhrlgiqa
fepvliegkaiqlhplvcaaynadfdgdqmavhvpltleaqlearalmmstnnilspang
epiivpsqdvvlglyymtrdcvnakgegmvltgpkeaerlyrsglaslharvkvriteye
kdangelvaktslkdttvgrailwmivpkglpysivnqalgkkaiskmlntcyrilglkp
tvifadqimytgfayaarsgasvgiddmvipekkheiiseaeaevaeiqeqfqsglvtag
erynkvidiwaaandrvskammdnlqtetvinrdgqeekqvsfnsiymmadsgargsaaq
irqlagmrglmakpdgsiietpitanfreglnvlqyfisthgarkgladtalktansgyl
trrlvdvaqdlvvteddcgthegimmtpvieggdvkeplrdrvlgrvtaedvlkpgtadi
lvprntllheqwcdlleensvdavkvrsvvscdtdfgvcahcygrdlarghiinkgeaig
viaaqsigepgtqltmaaaessiqvknkgsiklsnvksvvnssgklvitsrntelklide
fgrtkesykvpygavlakgdgeqvaggetvanwdphtmpvitevsgfvrftdmidgqtit
rqtdeltglsslvvldsaertaggkdlrpalkivdaqgndvlipgtdmpaqyflpgkaiv
qledgvqissgdtlaripqesggtkditgglprvadlfearrpkepailaeisgivsfgk
etkgkrrlvitpvpyeemipkwrqlnvfegervergdvisdgpeaphdilrlrgvhavtr
yivnevqdvyrlqgvkindkhievivrqmlrkativnagssdflegeqveysrvkianre
leangkatysrdllgitkaslatesfisaasfqettrvlteaavagkrdelrglkenviv
grlipagtgyayhqdrmrrraag

  • Chain 'E':
    Sequence, based on SEQRES records:
    >7c97E (E:)
marvtvqdavekignrfdlvlvaarrarqmqvggkdplvpeendkttvialreieeglin
nqildvrerqeqqeqeaaelqavtaiaegrr

    Sequence, based on observed residues (ATOM records):
    >7c97E (E:)
arvtvqdavekignrfdlvlvaarrarqmqvggkdplvpeendkttvialreieeglinn
qildvrerqeqqeqeaael

  • Chain 'F':
    Sequence, based on SEQRES records:
    >7c97F (F:)
meqnpqsqlkllvtrgkeqgyltyaevndhlpedivdsdqiediiqmindmgiqvmeeap
daddlmlaentadedaaeaaaqvlssveseigrttdpvrmymremgtvelltregeidia
kriedginqvqcsvaeypeaitylleqydrveaeearlsdlitgfvdpnaeedlaptath
vgselsqedldddededeedgdddsadddnsidpelarekfaelraqyvvtrdtikakgr
shataqeeilklsevfkqfrlvpkqfdylvnsmrvmmdrvrtqerlimklcveqckmpkk
nfitlftgnetsdtwfnaaiamnkpwseklhdvseevhralqklqqieeetgltieqvkd
inrrmsigeakarrakkemveanlrlvisiakkytnrglqfldliqegniglmkavdkfe
yrrgykfstyatwwirqaitrsiadqartiripvhmietinklnrisrqmlqemgreptp
eelaermlmpedkirkvlkiakepismetpigddedshlgdfiedttlelpldsattesl
raathdvlagltareakvlrmrfgidmntdytleevgkqfdvtrerirqieakalrklrh
psrsevlrsfldd

    Sequence, based on observed residues (ATOM records):
    >7c97F (F:)
eigrttdpvrmymremgtvelltregeidiakriedginqvqcsvaeypeaitylleqyd
rveaeearlsdlitgfvddpelarekfaelraqyvvtrdtikataqeeilklsevfkqfr
lvpkqfdylvnsmrvmmdrvrtqerlimklcveqckmpkknfitlftgnetsdtwfnaai
amnkpwseklhdvseevhralqklqqieeetgltieqvkdinrrmsigeakarrakkemv
eanlrlvisiakkytnrglqfldliqegniglmkavdkfeyrrgykfstyatwwirqait
rsiadqartiripvhmietinklnrisrqmlqemgreptpeelaermlmpedkirkvlki
akepismetpigddedshlgdfiedttlelpldsatteslraathdvlagltareakvlr
mrfgidmntdytleevgkqfdvtrerirqieakalrklrhpsrsevlrsfld

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.

  • Chain 'I':
    Sequence, based on SEQRES records:
    >7c97I (I:)
mavaankrsvmtlfsgptdiyshqvrivlaekgvsfeiehvekdnppqdlidlnpnqsvp
tlvdreltlwesriimeylderfphpplmpvypvargesrlymhriekdwytlmntiing
saseadaarkqlreellaiapvfgqkpyflsdefslvdcylapllwrlpqlgiefsgpga
kelkgymtrvferdsflaslteaeremrlgrs

    Sequence, based on observed residues (ATOM records):
    >7c97I (I:)
aankrsvmtlfsgptdiyshqvrivlaekgvsfeiehvekdnppqdlidlnpnqsvptlv
dreltlwesriimeylderfphpplmpvypvargesrlymhriekdwytlmntiingsas
eadaarkqlreellaiapvfgqkpyflsdefslvdcylapllwrlpqlgiefsgpgakel
kgymtrvferdsflaslteaeremrl

  • Chain 'J':
    Sequence, based on SEQRES records:
    >7c97J (J:)
mavaankrsvmtlfsgptdiyshqvrivlaekgvsfeiehvekdnppqdlidlnpnqsvp
tlvdreltlwesriimeylderfphpplmpvypvargesrlymhriekdwytlmntiing
saseadaarkqlreellaiapvfgqkpyflsdefslvdcylapllwrlpqlgiefsgpga
kelkgymtrvferdsflaslteaeremrlgrs

    Sequence, based on observed residues (ATOM records):
    >7c97J (J:)
vmtlfsgptdiyshqvrivlaekgvsfeiehvekdnppqdlidlnpnqsvptlvdreltl
wesriimeylderfphpplmpvypvargesrlymhriekdwytlmntiingsaseadaar
kqlreellaiapvfgqkpyflsdefslvdcylapllwrlpqlgiefsgpgakelkgymtr
vferdsflaslteaeremrlg

  • Chain 'K':
    Sequence, based on SEQRES records:
    >7c97K (K:)
mqgsvteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteve
idgvlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgd
veivkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspv
eriaynveaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrdvrqp
evkeekpefdpillrpvddleltvrsanclkaeaihyigdlvqrtevellktpnlgkksl
teikdvlasrglslgmrlenwppasiade

    Sequence, based on observed residues (ATOM records):
    >7c97K (K:)
dpillrpvddleltvrsanclkaeaihyigdlvqrtevelkslteikdvlasrglslgmr
lenwppa