PDB entry 7brs

View 7brs on RCSB PDB site
Description: E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02
Class: hydrolase
Keywords: beta_galactosidase, fluorescent probe, hydrolase
Deposited on 2020-03-30, released 2021-03-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-05-12, with a file datestamp of 2021-05-07.
Experiment type: XRAY
Resolution: 2.67 Å
R-factor: N/A
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1024)
      • engineered mutation (538)
    Domains in SCOPe 2.08: d7brsa1, d7brsa2, d7brsa3, d7brsa4, d7brsa5
  • Chain 'B':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1024)
      • engineered mutation (538)
    Domains in SCOPe 2.08: d7brsb1, d7brsb2, d7brsb3, d7brsb4, d7brsb5
  • Chain 'C':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1024)
      • engineered mutation (538)
    Domains in SCOPe 2.08: d7brsc1, d7brsc2, d7brsc3, d7brsc4, d7brsc5
  • Chain 'D':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1024)
      • engineered mutation (538)
    Domains in SCOPe 2.08: d7brsd1, d7brsd2, d7brsd3, d7brsd4, d7brsd5
  • Heterogens: F4X, MG, NA, DMS, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7brsA (A:)
smtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngew
rfawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvpten
ptgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflr
agenrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsra
vleaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenp
klwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhe
hhplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeanieth
gmvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksv
dpsrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqy
ahamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpnd
rqfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmval
dgkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawq
qwrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqllt
plrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitt
ahawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwl
glgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfn
isrysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyql
vwcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >7brsA (A:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrd
qftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahaw
qhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgp
qenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisry
sqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcq
k

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7brsB (B:)
smtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngew
rfawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvpten
ptgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflr
agenrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsra
vleaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenp
klwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhe
hhplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeanieth
gmvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksv
dpsrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqy
ahamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpnd
rqfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmval
dgkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawq
qwrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqllt
plrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitt
ahawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwl
glgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfn
isrysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyql
vwcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >7brsB (B:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrapldn
digvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgktlfi
srktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypdrlt
aacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlmets
hrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7brsC (C:)
smtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngew
rfawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvpten
ptgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflr
agenrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsra
vleaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenp
klwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhe
hhplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeanieth
gmvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksv
dpsrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqy
ahamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpnd
rqfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmval
dgkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawq
qwrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqllt
plrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitt
ahawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwl
glgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfn
isrysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyql
vwcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >7brsC (C:)
tdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfawf
papeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgcy
sltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragenr
lavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravleae
vqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklwsa
eipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhplh
gqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvpm
nrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsrp
vqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyahamg
nslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfcm
nglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkpl
asgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrla
enlsvtlaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrap
ldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgkt
lfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypd
rltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlm
etshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7brsD (D:)
smtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngew
rfawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvpten
ptgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflr
agenrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsra
vleaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenp
klwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhe
hhplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeanieth
gmvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksv
dpsrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqy
ahamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpnd
rqfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmval
dgkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawq
qwrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqllt
plrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitt
ahawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwl
glgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfn
isrysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyql
vwcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >7brsD (D:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrdqftrap
ldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahawqhqgkt
lfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgpqenypd
rltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisrysqqqlm
etshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcqk